Co-efficient of variation, heritability, genetic advance and variability for ricebean (Vigna umbellata (Thunb.) genotypes under mid hill conditions of Uttarakhand

The present investigation was carried out to estimate the genetic parameters for ten traits in ricebean genotypes. Fourteen genotypes of ricebean (Vigna umbellata (Thunb.) were collected from different regions of country were evaluated for yield and its components. The study revealed that the phenotypic coefficient of variation (PCV) was higher than genotypic coefficient of variation (GCV) for all traits. The value of PCV and GCV was higher for seed yield/plant, 100 seed weight and number of pods/plant. Heritability values were high for all the traits except leaflet size and pod length. High genetic advance was observed for days to 50% flowering, number of pods and seeds /plant, days to maturity, suggest that scope for improvement of these characters through selection. High heritability coupled with high genetic advance was registered for 50% flowering, number of pods and seeds /plant indicate that the genetic variance for these traits are probably owing to their high additive gene effect and thus there is better scope for improvement of these traits through direct selection. Therefore selection based on these characters will bring the desired improvement in seed yield of ricebean. With respect to mean performance of genotype, PRR-2, PRR-1 and PRR-2011- 1 was found superior among all the genotypes

To investigate the relationship between substrates of the N-end rule pathway and genes regulated by ‘GCCGCC’ cis-elements in Arabidopsis thaliana

The N-end rule pathway of targeted proteolysis is regulated by Group VII ethylene response factors (ERFVII’s). The aim of this research work was to analyse the relationship between substrates (ERFVII’s) of the N-end rule pathway and genes, which have promoters containing a double ‘GCCGCC’ Ethylene-Responsive Element Binding Protein (EBP) cis-element. Several genes were identified containing double EBP elements. Cloning and transformation of the promoters from five of these genes (PYL, ERD4, AT1G14810, AT3G13440 and AT5G44420) carrying two copies of the GCC-boxes present in the 5' UTR (5’ untranslated region) or promoter region was conducted into Arabidopsis wild-type (Col-0) and prt6-1 mutant plants. Expression driven by these promoters in the leaves and flowers of transgenic plants was analysed through GUS staining to reveal promoter activities. Enhanced promoter activity was seen in prt6-1 lines (mutated in the E3-ligase of the N-end rule pathway) in comparison to Col-0. Further, cDNA of leaves and flowers of Col-0 and prt6-1 were analysed by q-RT-PCR (quantitative real-time PCR) for expression of PYL7, ERD4, AT1G14810, AT3G13440; t-test analysis showed a significant difference (p-value<0.05) only in leaves of Col-0 and prt6-1 for PYL7. Analysis of the genetic relationship between N-end rule pathway and genes containing GCC-boxes was also performed by analysing double mutant combinations of prt6-1 and mutants of genes containing the EBP elements (pyl7prt6-1, erd4prt6-1 and abi5prt6-1) and Col-0 under different concentration of salt to determine the effect of stress due to salinity on the regulation of genes. At 125mM salt concentration significant difference was identified in highest number of mutant lines in comparison to Col-0. An analysis of the in-vivo binding of the ERFVII RAP2.3 to the promoter of GCC-boxes containing genes was performed through Chromatin Immuno-precipitation assay (ChIP). The t-test analysis on qChIP-PCR data indicated significant difference between IgG and HA-IPs for both ABI5 and PYL7 performed on normoxic 35S:MA-RAP2.3-HA in Col-0 line. Further, in-vivo localization of ERFVII’s HRE1 and RAP2.2 conditional stability was analysed using promERFVII’s:MC/MA-ERFVII’s-YFP constructs in Col-0 and prt6-1. This thesis suggests that genes PYL7, ERD4, AT1G14810, AT3G13440 that have double ‘GCCGCC’ EBP elements are downstream targets of the N-end rule pathway. Further analysis via ChIP suggests that RAP2.3 interacts with the ‘GCCGCC’ binding site of promoters in the ABI5 and PYL7 genes, however further work is needed to confirm this. Additionally, sub-cellular localization of promERFVII’s:MC/MA-ERFVII’s-YFP studies suggest the location of HRE1 and RAP2.2 in nuclei of early stage root tips studied on 4-days old etiolated seedlings

Anaemia in acute coronary syndrome patients: a study from rural tertiary care centre of India

Background: Acute coronary syndromes (ACS) are an imbalance between myocardial oxygen supply and demand, and the presence of anaemia further potentiates this imbalance. The burden of anaemia in patients presenting with acute coronary syndromes (ACS) is significant. Anaemia has the potential to worsen myocardial ischemic insult by decreasing the oxygen content of the blood supplied to the jeopardized myocardium.Methods: A total of 148 patients with ACS were recruited in the study from October 2016 to December 2017 in Medicine and Cardiology Department of UPUMS Saifai, India. All patients were subjected to a detailed history and thorough clinical examination and investigations after obtaining informed consent. Patient having any other diseases known to cause anaemia were excluded.Results: Mean age of patients was 58.5 years. 72.97% were vegetarian and 27.03% were non-vegetarian. Most common morphological type of anaemia was dimorphic anaemia followed by macrocytic and microcytic hypochromic respectively. Iron deficiency anaemia was most common type of anaemia followed by vitamin B12 deficiency and mixed (Iron and vitamin B12 deficiency). 45.28% anaemic patients had no symptoms of blood loss. Most common symptom of blood loss was bleeding per rectum followed by malena. Severity of acute coronary syndrome was more in subjects having anaemia which was evident by higher incidence of anaemia in subjects having ST elevation myocardial infarction (STEMI). The incidence of anaemia was low in case of Non ST elevation Myocardial Infarction (NSTEMI) and Unstable angina (UA). The results of the present study have been compared to those from India.Conclusions: Higher incidence of anaemia was reported in subjects having acute coronary syndrome. Incidence of anaemia in STEMI patients was greater than NSTEMI and unstable angina patients. Severe form of acute coronary syndrome i.e. STEMI was associated with higher incidence of anaemia.

Influenza B virus: Need for heightened surveillance and epidemiologic case studies

Recent report of increased influenza B virus infection, particularly theclinical profiles and treatment challenges imposed like that of influenza A,underscores the importance of continuing influenza B virus surveillance.This is, especially in resource limited country, early detection of influenzavirus, its clinical presentation and complications would be vital in minimizingthe public heath burden imposed by this virus.Keywords: chronic obstructive pulmonary disease, influenza B, severe acutepulmonary infection

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