Knockdown of LncRNA SBF2-AS1 Inhibited Gastric Cancer Tumorigenesis via the Wnt/LRP5 Signaling Pathway

This investigation aimed to uncover the impact of a long noncoding RNA, SET-binding factor 2 antisense RNA1 (SBF2-AS1) on the malignant progression of gastric cancer (GC) and to further explore its underlying mechanism. SBF2-AS1 expression was quantified by qRT-PCR in GC cell lines and GC tissues. In vitro loss-of-function studies of SBF2-AS1, accompanied by flow cytometry, CCK-8, and cell invasion tests, were applied to elucidate the impact of SBF2-AS1 on the tumor progression of GC cells. Finally, Western blotting and a luciferase assay were used to detect WNT/LRP5 signaling pathway activation. SBF2-AS1 was aberrantly expressed in GC cell lines (p<0.05) and GC tissues (p<0.05). Cell invasive and proliferative capabilities were inhibited via SBF2-AS1 knockdown, resulting in apoptosis of NCI-N87 and MKN74 cells. Additionally, online database analysis uncovered a positive correlation between SBF2-AS1 and the Wnt/LRP5 signaling pathway (p<0.05). SBF2-AS1 knockdown blocked the Wnt/LRP5 signaling pathway, whereas the effects of SBF2-AS1 knockdown on the malignant genotype of MKN74 as well as NCI-N87 cells were partially restored by triggering the Wnt/ LRP5 signaling pathway. High expression of SBF2-AS1 was found in GC, the malignant progression of which was repressed via SBF2-AS1 knockdown by inhibiting the Wnt/LRP5 signaling pathway

Insights into the issue in IPv6 adoption: a view from the Chinese IPv6 Application mix

Published onlineThis is the author accepted manuscript. The final version is available from Wiley via the DOI in this record.Although IPv6 has been standardized more than 15 years ago, its deployment is still very limited. China has been strongly pushing IPv6, especially due to its limited IPv4 address space. In this paper, we describe measurements from a large Chinese academic network, serving a significant population of IPv6 hosts. We show that despite its expected strength, China is struggling as much as the western world to increase the share of IPv6 traffic. To understand the reasons behind this, we examine the IPv6 applicative ecosystem. We observe a significant IPv6 traffic growth over the past 3 years, with P2P file transfers responsible for more than 80% of the IPv6 traffic, compared with only 15% for IPv4 traffic. Checking the top websites for IPv6 explains the dominance of P2P, with popular P2P trackers appearing systematically among the top visited sites, followed by Chinese popular services (e.g., Tencent), as well as surprisingly popular third-party analytics including Google. Finally, we compare the throughput of IPv6 and IPv4 flows. We find that a larger share of IPv4 flows get a high-throughput compared with IPv6 flows, despite IPv6 traffic not being rate limited. We explain this through the limited amount of HTTP traffic in IPv6 and the presence of Web caches in IPv4. Our findings highlight the main issue in IPv6 adoption, that is, the lack of commercial content, which biases the geographic pattern and flow throughput of IPv6 traffic. Copyright © 2014 John Wiley & Sons, Ltd

Electrical‐Triggered Multicolor Reversible Color‐Changing Ag Nanoparticles/Reduced Graphene Oxide/Polyurethane Conductive Fibers

Abstract Smart color‐changing fibers attract much attention owing to their importance as a component of flexible electronics. A facile and scalable method of multicolor reversible electro–thermochromic Ag nanoparticles/reduced graphene oxide/polyurethane conductive fiber (ETC AgNPs/rGO/PU conductive fiber) is fabricated, which contains the polyurethane (PU) as the inner layer, reduced graphene oxide (rGO) with Ag nanoparticles (AgNPs) as the conductive layer, and thermochromic paste as the outermost layer. It possesses excellent electrothermal and color‐changing properties and rapidly generates Joule heat at 0.5 V, which makes the fiber surface temperature reach 39.81 °C rapidly. The color switching rate is fast and changes from green to yellow within 2 s. During the process of 250 times on/off voltage, ETC AgNPs/rGO/PU conductive fibers still maintain excellent electrical and thermal properties and color change stability; even in the washing, strong acid, and strong alkali environment, they still have excellent durability. This human subjective adjustable electrical–thermal–color multi‐level induced modulation makes it possible to be applied to smart wearable fields such as visual camouflage, personal thermal management, and active information transfer

Rabeprazole combined with hydrotalcite is effective for patients with bile reflux gastritis after cholecystectomy

BACKGROUND: Regardless of surgical technique, patients who have undergone cholecystectomy appear to be predisposed to the development of bile reflux gastritis

TRIM47 is up-regulated in colorectal cancer, promoting ubiquitination and degradation of SMAD4

Abstract Background Tripartite motif 47 (TRIM47), a member of the TRIM family proteins, plays a key role in many types of cancers including colorectal cancer (CRC). We found that levels of TRIM47 mRNA and protein were increased significantly in colorectal tumors compared with nontumor tissues and the increased levels were associated with advanced tumor stage and poor outcome. Methods We used quantitative polymerase chain reaction and western blot to measure levels of TRIM47 mRNA and protein in human colorectal cancer and paired normal tissues. TRIM47 was knocked down and overexpressed in colorectal cancer cells, and the effects on cell proliferation, migration and growth of xenograft tumors in nude mice were assessed. The signaling pathways were examined by western blot and immunoprecipitation assays. Results TRIM47 promoted CRC proliferation and metastasis in vitro and in vivo as an oncogene. Mechanistically, TRIM47 interacted physically with SMAD4, increasing its ubiquitination and degradation. Loss of SMAD4 leaded to up-regulation of CCL15 expression and caused growth and invasion in human CRC cells through the CCL15-CCR1 signaling. Moreover, TRIM47 overexpression played a role in CRC chemoresistance in response to 5-FU therapy. Conclusions Our study demonstrated a functional role of the TRIM47-SMAD4-CCL15 axis in CRC progression and suggested a potential target for CRC therapy

Interleukin-23R rs7517847 T/G Polymorphism Contributes to the Risk of Crohn’s Disease in Caucasians: A Meta-Analysis

The association between Interleukin-23R gene polymorphism and Crohn’s disease (CD) in Caucasians is still controversial. Thus, a meta-analysis was performed to evaluate the correlation between this gene variant and CD risk. We retrieved the available data from EMBASE and PUBMED until May 1, 2014, and evaluated the effect of rs7517847 in Caucasians. The significant associations were confirmed between rs7517847 and CD risk in dominant models (TT/TG versus GG: OR = 1.652, 95% CI 1.277, 2.137), allelic model (T allele versus G allele: OR = 1.327, 95% CI 1.198, 1.469), homozygote comparison (TT versus GG: OR = 1.890, 95% CI 1.465, 2.437), heterozygote comparison (TG versus GG: OR = 1.509, 95% CI 1.161, 1.960), and recessive model (TT versus TG/GG: OR = 1.409, 95% CI 1.279, 1.552). In conclusion, this meta-analysis demonstrates that rs7517847 is associated with the risk of CD in Caucasians. These findings show that IL-23R genes confer susceptibility to CD in the Caucasians