Development, chemometric-assisted optimization and in-house validation of a modified pressurized hot water extraction (PHWE) method for multi-mycotoxin analysis

Abstract : Please refer to full text to view abstract.D.Tech. (Food Technology

Food Metabolomics: A New Frontier in Food Analysis and its Application to Understanding Fermented Foods

The emergence of food metabolomics, otherwise known as foodomics, has opened new frontiers and possibilities for scientists to characterize and simultaneously determine and obtain the comprehensive profile of the food metabolome. Qualitative and quantitative determinations of this metabolome offer insights into the underlying processes involved and details about the content of the food analytes. This had seemed technically challenging and impossible over time, but can now be done due to the advent of sophisticated analytical equipment and chemometric tools. The application of this technique offers enormous opportunities to obtain detailed information that can be correlated to various properties, functionalities and potentials in fermented foods. This chapter thus evaluated and documented studies presented in the literature on the food metabolomics study of fermented foods, with a view of appraising its prospects, applications and subsequent utilization in the study of fermented foods

Fermented Pulse-Based Food Products in Developing Nations as Functional Foods and Ingredients

Pulses play a significant and diverse role in the agricultural systems and diets of underprivileged populations worldwide. They are ideal produce for reducing poverty, improving human health and nutrition, and enhancing resilience of the ecosystem. Fermentation is a processing technique that has been used for decades to transform food produce with improved health, functional, and nutraceutical benefits. In tandem with the United Nations’ (UN’s) sustainable development goal Number 3, fermented food products from pulses with health benefits align with this initiative to end hunger, achieve food security, and improve nutrition. In solidarity with the celebration of International Year of Pulses 2016 (IYP2016) and considering the relative neglect of pulses as compared with other food groups, this chapter would be vital in positioning pulses and fermented products from them as readily available functional foods. With increased interest in fermentation, fermented pulse-based foods have been identified as excellent sources of bioactive and functional foods. Thus, fermented pulse-based products present a viable alternative, relatively available, affordable, and cheap source of foods with properties beyond that of basic nutrition

Parallel validation of a green-solvent extraction method and quantitative estimation of multi-mycotoxins in staple cereals using LC-MS/MS

In this study, 15 different mycotoxins were estimated in three staple cereals from selected agro-ecological regions in Nigeria using a 'novel' green extraction method, pressurized hot water extraction (PHWE) in comparison to a conventional solvent extraction method. Discrimination of the results of PHWE and solvent extraction using principal component analysis (PCA) and orthogonal projection to latent structures discriminate analysis (OPLS-DA) did not yield any differential clustering patterns. All maize samples (n=16), 32% (n=38) of sorghum and 35% (n=37) of millet samples were positive for at least one of the 15 tested mycotoxins. Contamination levels for the cereals were higher in the warm humid rain forest region and gradually decreased towards the hot and arid region in the north of the country. The results demonstrate the applicability of PHWE as a possible alternative extraction method to conventional methods of extraction, which are solvent based

Metabolite profile of raw and cooked pasta from whole wheat grain enriched with Bambara groundnut.

This study assessed the metabolite profile of raw and cooked pasta from whole wheat grain enriched with Bambara groundnut using gas chromatography high-resolution time-of-flight mass spectrometry (GC-HRTOF-MS). Different metabolites (49), such as alcohols, amides, aldehydes, amines (4%), aromatic compounds (6%), fatty acids (4%), fatty acid ethyl ester (4%), ketones (6%), lipids (2%), nucleobase (2%), phenolic compounds (4%), plasticizer (2%), steroids, and terpenes (4%), were found in the pasta samples. Ester compounds (ethyl ester, fatty acid ethyl ester, and fatty acid methyl ester), miscellaneous compounds, and metabolites with pharmaceutical properties accounted for the bulk of the metabolites in the pasta samples. Uncooked pasta showed lower amounts (i.e., less diversity) of metabolites (10) compared with their cooked counterparts (21), but 18 similar metabolites were found in both cooked and uncooked pasta. Changes in the metabolite composition could be associated with transformation during cooking since a larger number of metabolites were formed after cooking. This study would guide future utilization of pulse-enriched pasta and provide direction for further research in the metabolomic research space

Application of a generative adversarial network for multi-featured fermentation data synthesis and artificial neural network (ANN) modeling of bitter gourd–grape beverage production.

Artificial neural networks (ANNs) have in recent times found increasing application in predictive modelling of various food processing operations including fermentation, as they have the ability to learn nonlinear complex relationships in high dimensional datasets, which might otherwise be outside the scope of conventional regression models. Nonetheless, a major limiting factor of ANNs is that they require quite a large amount of training data for better performance. Obtaining such an amount of data from biological processes is usually difficult for many reasons. To resolve this problem, methods are proposed to inflate existing data by artificially synthesizing additional valid data samples. In this paper, we present a generative adversarial network (GAN) able to synthesize an infinite amount of realistic multi-dimensional regression data from limited experimental data (n = 20). Rigorous testing showed that the synthesized data (n = 200) significantly conserved the variances and distribution patterns of the real data. Further, the synthetic data was used to generalize a deep neural network. The model trained on the artificial data showed a lower loss (2.029 ± 0.124) and converged to a solution faster than its counterpart trained on real data (2.1614 ± 0.117)

Subcritical Water Extraction and Its Prospects for Aflatoxins Extraction in Biological Materials

Aflatoxins (AFs) are well-known mycotoxins and contaminants of various agricultural commodities globally that are linked to a wide range of adverse health and economic complications. Because of their incessant proliferation and deleterious consequences, it has become mandatory to routinely monitor the levels of these toxins in agricultural products before they go into the market. Essentially, effective analysis is an important component of AFs control, and extraction is a necessary step for their analysis, irrespective of the protocol adopted. Conventional methods for AF extraction are expensive, the processes involved are tedious and utilize large quantities of organic solvents that are environmentally unfriendly. This has necessitated the quest for alternatives that are ‘green’, cost-effective and easy to perform. In this regard, subcritical water extraction (SWE) is a viable alternative that has proven to be effective in the extraction of other bioactive compounds. This chapter presents a critical appraisal of the principles and dynamics of SWE, and its current applications as a viable tool in the extraction of AFs from various biological matrices. Although further research needs to be performed to enhance its applicability, the adoption of SWE in the extraction of AFs seems very promising and needs to be properly exploited

Mitigation of Acrylamide in Foods: An African Perspective

Acrylamide (ACR) is a possible human carcinogen, with neurotoxic properties. It is a heat-generated food toxicant particularly found in carbohydrate-rich foods. Its occurrence is of global concern and constitutes a major challenge to food safety, due to its presence in several thermally processed foods worldwide. Since its discovery, ACR has been recognized as one of the most widely investigated heat-induced food contaminant, and several reports on its formation and occurrence since its discovery have been reported. However, information on the extent of ACR occurrence in foods consumed in different parts of Africa is rather too limited. This is particularly a concern considering that most carbohydrate-based foods, subjected to varying degrees of thermal processing, are consumed as staple diets almost on daily basis in the continent. As such, African populations may be exposed to high levels of ACR daily. Thus, this chapter covers the formation, occurrence and health impact of ACR in foods. It further summarizes previous studies looking at ACR reduction and mitigation strategies, especially those that may be applicable in the continent. Adequate sensitization of the populace about the prevention of ACR as a food contaminant is essential to ensure the safety of heat-processed carbohydrate-rich foods in the continent

The Socio-Economic Impact of Mycotoxin Contamination in Africa

The proliferated contamination of agricultural commodities by mycotoxins and their attendant toxic effects on humans and animals which consume such commodities constitutes a major concern to food safety and security. These highly toxic food contaminants are produced by various filamentous fungi species that are ubiquitous in nature, however, favourable climatic conditions in the tropics favour their proliferation in these regions. Africa, by virtue of its location along the equator makes it highly accommodative to proliferation of mycotoxigenic fungi species, as such, it is the most affected of all the continents. Other factors such as poverty, and climate change further complicates the mycotoxin situation on the continent. Economic impact due to mycotoxin contamination in Africa is thus alarming. The effects of mycotoxins can in fact be felt in the overall health of humans and animals, sustainable development, food security and safety, damage to the African agricultural export brand, negatively impacting Africa’s self-sustainability and increased dependence on foreign aid, not excluding high cost of research, mitigation and regulation of the prevalence of these toxins in African countries. This book chapter presents an exhaustive appraisal of the socio-economic impact of mycotoxins on Africa. Our observations herein are expected to stimulate policy makers, as well as, all stakeholders along the food supply chain to identify critical areas of collaboration and strengthen alliances in order to ameliorate the effects of these toxicants on the continent of Africa, and the world at large

Decontamination of T-2 Toxin in Maize by Modified Montmorillonite Clay

Montmorillonite clay has a wide range of applications, one of which includes the binding of mycotoxins in foods and feeds through adsorption. T-2 toxin, produced by some Fusarium,Myrothecium, and Stachybotrys species, causes dystrophy in the brain, heart, and kidney. Various formulations that include lemongrass essential oil-modified montmorillonite clay (LGEO-MMT), lemongrass powder (LGP), montmorillonite clay washed with 1mMNaCl (Na-MMT),montmorillonite clay (MMT), and lemongrass powder mixed with montmorillonite clay (LGP-MMT) were applied to maize at concentrations of 8% and 12% and stored for a period of onemonth at 30 �C. Unmodifiedmontmorillonite clay and LGP served as the negative controls alongside untreated maize. Fourier Transform Infrared (FTIR) spectra of the various treatments showed themajor functional groups as Si-O and -OH. All treatment formulationswere effective in the decontamination of T-2 toxin inmaize. Accordingly, itwas revealed that the inclusion ofNa-MMT in maize atMontmorillonite clay has a wide range of applications, one of which includes the binding of mycotoxins in foods and feeds through adsorption. T-2 toxin, produced by some Fusarium,Myrothecium, and Stachybotrys species, causes dystrophy in the brain, heart, and kidney. Various formulations that include lemongrass essential oil-modified montmorillonite clay (LGEO-MMT), lemongrass powder (LGP), montmorillonite clay washed with 1mMNaCl (Na-MMT),montmorillonite clay (MMT), and lemongrass powder mixed with montmorillonite clay (LGP-MMT) were applied to maize at concentrations of 8% and 12% and stored for a period of onemonth at 30 �C. Unmodifiedmontmorillonite clay and LGP served as the negative controls alongside untreated maize. Fourier Transform Infrared (FTIR) spectra of the various treatments showed themajor functional groups as Si-O and -OH. All treatment formulationswere effective in the decontamination of T-2 toxin inmaize. Accordingly, itwas revealed that the inclusion ofNa-MMT in maize at a concentration of 8% was most effective in decontaminating T-2 toxin by 66% in maize followed by LGP-MMT at 12% inclusion level recording a 56% decontamination of T-2 toxin in maize (p = 0.05). Montmorillonite clay can be effectivelymodified with plant extracts for the decontamination of T-2 toxin a concentration of 8% was most effective in decontaminating T-2 toxin by 66% in maize followed by LGP-MMT at 12% inclusion level recording a 56% decontamination of T-2 toxin in maize (p = 0.05). Montmorillonite clay can be effectivelymodified with plant extracts for the decontamination of T-2 toxi