Development of the dog attachment insecurity screening inventory (D-aisi):A pilot study on a sample of female owners

SIMPLE SUMMARY: The Strange Situation Procedure is a laboratory test originally designed to assess the quality of a child’s attachment bond to their mother and is widely used in dogs to assess their attachment bond towards the owner. However, the SSP is time consuming and limits the amount and variety of obtainable data. In order to overcome these limitations, we adapted a three-dimensional parent-report scale, named the Attachment Insecurity Screening Inventory (AISI) 6–12, originally developed to assess 6- to 12-year-old children’s attachment insecurity, to dog–owner dyads, and we assessed scale consistency and validity. A first statistical analysis performed on the responses provided by 524 female owners to the online questionnaire revealed five scale dimensions named “physical contact”, “control”, separation anxiety”, “owner as emotional support”, and “owner as a source of positive emotion”. However, a further forced extraction of three components resulted in subscales that mirrored the ones reported for the original AISI in terms of item composition (i.e., ambivalent, avoidant, and disorganized). The three subscales also had satisfactory to good measures of internal reliability. The final scale was named the Dog Attachment Insecurity Screening Inventory (D-AISI). Although promising, it needs to be refined and tested for more validity measures. ABSTRACT: To date, the Strange Situation Procedure is the only tool available to investigate the quality of the dog’s attachment bond towards the owner. This study aimed to adapt a parent-report scale, named the Attachment Insecurity Screening Inventory (AISI) 6–12, originally designed to assess 6- to 12-year-old children’s attachment insecurity, to dog–owner dyads and assess measures of consistency and validity. The online questionnaire was completed by 524 female dog owners. Principal component analysis (PCA) revealed five components named, respectively, “physical contact”, “control”, “separation anxiety”, “owner as emotional support”, and “owner as a source of positive emotion”. Because of the three-factor structure of the original AISI, a PCA with a pre-fixed set of three factors was also performed. The resulting subscales mirrored the ones found for the original scale (i.e., ambivalent, avoidant, and disorganized), although four items did not fit the model. Internal reliability appeared to be satisfying for the ambivalent and the disorganized subscales, and good for the avoidant subscale. The theoretical background and the results of this study suggest that the three-dimensional model represents a better solution for the interpretation of the Dog Attachment Insecurity Screening Inventory (D-AISI). Although promising, this scale requires refinement and assessment of additional validity measures

Strontium-Substituted α-TCP: Structure, Stability, and Reactivity in Solution

& alpha;-Tricalcium phosphate (& alpha;-TCP) is widelyused as acomponent of bone cements, and many efforts have been made to dopeit with strontium ion (Sr), which is known for its beneficial rolein bone tissue. However, the range of possible substitution of strontiumfor calcium (Ca) into & alpha;-TCP, as well as its effect on the & alpha;-TCPstructure, has not been clarified yet. Herein, we investigate thissubstitution through the examination of & alpha;-TCP synthesized athigh temperatures in the presence of increasing amounts of strontiumaccording to two different routes: 1-step and 2-steps. The results show that Sr can enter into an & alpha;-TCPstructure up to about 10 atom % and substitutes for calcium mostlyat specific cation sites, namely, M(5), M(11), and M(17), characterizedby relatively low bond valence sums and long mean Ca-O distance.Strontium presence stabilizes & alpha;-TCP delaying its transformationinto octacalcium phosphate and hydroxyapatite in H3PO4, as well as in physiological solution. Although the two methodsof synthesis provide similar structural results, the products of 1-step synthesis display a slightly smaller crystallitesize and greater solubility and, as a consequence, a faster hydrolysisreaction.& alpha;-Tricalcium phosphatepreparation through the solid-statereaction is described following two different procedures. The investigationof the range of possible substitution of strontium for calcium into & alpha;-TCP as well as its effect on the & alpha;-TCP structure ispresented. Furthermore, it is shown that strontium presence stabilizes & alpha;-TCP and delays its hydrolysis reaction into octacalcium phosphateand hydroxyapatite

Synthesis and Hydrolysis of Brushite (DCPD): The Role of Ionic Substitution

Brushite (dicalcium phosphate dihydrate, DCPD) is considered one of the possible precursors of the apatitic phase that constitutes the mineral component of bones, and it is often utilized in the preparation of biomaterials for hard tissue repair. In this work, we investigated the influence of ionic substitution on the synthesis, structure, and morphology of this calcium phosphate, as well as on its hydrolysis process. The results of structural refinements indicate that the range of possible substitution can reach values up to about 38 atom % for the big Sr ion, whereas it is quite limited for Zn, Co, and Mn. In particular, DCPD cannot be obtained as a single phase in the presence of zinc ions in solution. The kind and amount of substituent ions significantly influence the morphology of DCPD, promoting aggregation and crystal shape modifications, as well as its hydrolysis in solution. The results provide useful information for the understanding of the mineralization processes and for the design of new biomaterials

Poly(Alkylene 2,5-thiophenedicarboxylate) polyesters: A new class of bio-based high-performance polymers for sustainable packaging

In the present study, 100% bio-based polyesters of 2,5-thiophenedicarboxylic acid were synthesized via two-stage melt polycondensation using glycols containing 3 to 6 methylene groups. The so-prepared samples were characterised from the molecular point of view and processed into free-standing thin films. Afterward, both the purified powders and the films were subjected to structural and thermal characterisation. In the case of thin films, mechanical response and barrier properties to O2 and CO2 were also evaluated. From the results obtained, it emerged that the length of glycolic sub-units is an effective tool to modulate the chain mobility and, in turn, the kind and amount of ordered phases developed in the samples. In addition to the usual amorphous and 3D crystalline phases, in all the samples investigated it was possible to evidence a further phase characterised by a lower degree of order (mesophase) than the crystalline one, whose amount is strictly related to the glycol sub-unit length. The relative fraction of all these phases is responsible for the different mechanical and barrier performances. Last, but not least, a comparison between thiophene-based homopolymers and their furan-based homologues was carried out

The assessment of dog welfare in the waiting room of a veterinary clinic

Veterinary visits are known to be stressful for many dogs. The aim of this study was to assess dog welfare in the waiting room of the veterinary clinic through a multi-modal, non-invasive approach. Forty-five dogs were each videoed for 3 min in the waiting room of a veterinary clinic where they went for a scheduled visit. The welfare of each dog was assessed using a thorough video analysis and two overall evaluations (low, medium and high stress); one performed by a veterinary behaviourist and one by the dog's owner. Two-thirds of dogs spent more than 20% of the time displaying at least one indicator of stress, and 53.3% showed four or more behavioural signs of stress. Assessments of stress by the behaviourist indicated that level of stress in the waiting room was high in 28.9% of cases. The agreement between owners' and behaviourist's overall evaluations was quite low. The behaviourist's evaluations were strongly correlated with the time spent by dogs showing signs of stress and moderately correlated with the number of displayed signs, whilst owners' evaluations were not closely correlated to those factors. Dogs rated as highly stressed by the behaviourist were more prone to display resistance (halting, refusing to budge) when moving from the waiting room to the consultation room. The results of this pilot study support the idea that the welfare of dogs in the veterinary waiting room is often impaired, and that owners are unable to accurately assess stress in their dogs in such situations

Adaptive mechanisms in dogs adopted from shelters: A behavioral assessment of the use of a synthetic analogue of the canine appeasing pheromone

Adaptation to a new socio-environment might represent a very hard step for sheltered dogs, because of a higher level of difficulty in coping with unfamiliar conditions. The adaptation process can be logically and scientifically related to the concept of stress and welfare, limiting the success rate of rehoming. A synthetic analogue of the Dog Appeasing Pheromone (Dog Appeasing Pheromone, DAP) is reported to have a reassuring effect in puppies and adult dogs in a wide variety of stressful situations. The aim of the current study was to investigate the effects of DAP (Adaptil® Ceva Vetem S.p.A.) in dogs re-homed from rescue shelters. The study was designed as a prospective open-label clinical trial. Significant decreases were observed in adult dogs for wandering in the house restlessly (p=0.022) and hiding fearfully in protected corners (p=0.033), whereas in puppies treatment with DAP significantly (p<0.05) improved the reaction towards unfamiliar dogs (p=0.048) and wandering in the house restlessly (p=0.022). In both adults and puppies a significant improvement in interaction with owners was observed. In particular, "looking continuously for the owners" and "following the owners everywhere like a shadow" were significantly improved (p=0.0012 and 0.0016 respectively) in adult dogs. Separation reactions revealed a significant decrease (p<0.05) and in puppies the tendency to vocalize in absence of the owner was also significantly reduced (p=0.0029). Both adults and puppies showed a decreased tendency to wake suddenly in the night (p=0.024 and p=0.026 respectively) and wander around the home (p=0.012 and p=0.026 respectively). In contrast, for house-training no significant difference was reported in adults, whereas for puppies there was a significant decrease (p<0.05) in the mean scores for urination and/or defecation wherever in the house and after coming home. Data regarding the overall assessment suggested a significant improvement in all the efficacy variables considered in the study. The analysis of owners' degree of satisfaction at the final visit showed that DAP treatment was considered successful by 84.4 % of owners. Results suggest that DAP might improve dogs' adaptability throughout the first weeks following adoption and can be considered a useful tool for reducing stress in re-homed dogs

A time-temperature integrator based on fluorescent and polymorphic compounds

Despite the variety of functional properties of molecular materials, which make them of interest for a number of technologies, their tendency to form inhomogeneous aggregates in thin films and to self-organize in polymorphs are considered drawbacks for practical applications. Here, we report on the use of polymorphic molecular fluorescent thin films as time temperature integrators, a class of devices that monitor the thermal history of a product. The device is fabricated by patterning the fluorescent model compound thieno(bis)imide-oligothiophene. The fluorescence colour of the pattern changes as a consequence of an irreversible phase variation driven by temperature, and reveals the temperature at which the pattern was exposed. The experimental results are quantitatively analysed in the range 20–200°C and interpreted considering a polymorph recrystallization in the thin film. Noteworthy, the reported method is of general validity and can be extended to every compound featuring irreversible temperature-dependent change of fluorescence

Identification of redox-sensitive transcription factors as markers of malignant pleural mesothelioma.

Although asbestos has been banned in most countries around the world, malignant pleural mesothelioma (MPM) is a current problem. MPM is an aggressive tumor with a poor prognosis, so it is crucial to identify new markers in the preventive field. Asbestos exposure induces oxidative stress and its carcinogenesis has been linked to a strong oxidative damage, event counteracted by antioxidant systems at the pulmonary level. The present study has been focused on some redox-sensitive transcription factors that regulate cellular antioxidant defense and are overexpressed in many tumors, such as Nrf2 (Nuclear factor erythroid 2-related factor 2), Ref-1 (Redox effector factor 1), and FOXM1 (Forkhead box protein M1). The research was performed in human mesothelial and MPM cells. Our results have clearly demonstrated an overexpression of Nrf2, Ref-1, and FOXM1 in mesothelioma towards mesothelium, and a consequent activation of downstream genes controlled by these factors, which in turn regulates antioxidant defense. This event is mediated by oxidative free radicals produced when mesothelial cells are exposed to asbestos fibers. We observed an increased expression of Nrf2, Ref-1, and FOXM1 towards untreated cells, confirming asbestos as the mediator of oxidative stress evoked at the mesothelium level. These factors can therefore be considered predictive biomarkers of MPM and potential pharmacological targets in the treatment of this aggressive cancer