Inclusive photon production and photon-jet correlations in hadronic collisions

We compare results of $k_t$-factorization approach and next-to-leading order collinear-factorization approach for photon-jet correlations in $pp$ and $p \bar p$ collisions at RHIC, Tevatron and LHC energies. We discuss correlations in azimuthal angle between photon and jet as well as correlations in two-dimensional space of photon and jet transverse momenta. Different unintegrated gluon/parton distributions are used in the $k_t$-factorization approach. The results depend on UGDF/UPDF used. The collinear NLO $2 \to 3$ contributions dominate over $k_t$-factorization cross section at small relative azimuthal angles as well as for asymmetric transverse momentum configurations.Comment: 6 pages, 7 figures a talk at the International Workshop PHOTON2007, Paris, Sorbonne France, July 9-13, 200

Pion and Kaon Production in Nucleon - Nucleon Collisions

Inclusive cross section for pion production in proton - proton collisions are calculated based on unintegrated parton distribution functions (uPDFs). In addition to purely gluonic terms the present approach includes also quark degrees of freedom. Phenomenological fragmentation functions from the literature are used. The new mechanisms are responsible for $\pi^+$ - $\pi^-$ asymmetry. In contrast to standard collinear approach, application of 2 $\to$ 1 $k_t$ - factorization approach can be extended towards much lower transverse momenta, both at mid and forward rapidity region. The results of the calculation are compared with SPS and RHIC data.Comment: a talk presented by Marta Tichoruk at the international conference MESON2006, Cracow, June 2006, 5 pages, 3 figure

Cell death in HeLa cells upon imperatorin and cisplatin treatment

There is growing evidence that commonly applied chemotherapy regimens can be improved by introducingnew, specific, active and low side-effect drugs, or by combining substances to obtain the required clinicaleffect. The aim of the present study was to investigate the effects of imperatorin and cisplatin, applied separatelyor in combination, on apoptosis, necrosis and autophagy induction in the human cervical carcinoma cell line(HeLa). Imperatorin appeared to be a potent autophagy inducer, rather than a necrotic or apoptotic one. Incontrast, cisplatin induced mainly apoptosis and necrosis after 6 h and 24 h, while longer incubation resultedonly in necrosis induction. When HeLa cells were incubated with both drugs, autophagy appeared most frequently,although to a smaller extent than that observed after imperatorin administered alone. At the molecularlevel, autophagy was correlated with the presence of the cleaved form of microtubule-associated protein 1 lightchain LC3 — LC3II. It was also accompanied by the inhibition of heat shock proteins Hsp27 and Hsp72 expression.Our results indicate that imperatorin alone, or in combination with cisplatin, is mainly an autopahgy inducerin HeLa cells.There is growing evidence that commonly applied chemotherapy regimens can be improved by introducingnew, specific, active and low side-effect drugs, or by combining substances to obtain the required clinicaleffect. The aim of the present study was to investigate the effects of imperatorin and cisplatin, applied separatelyor in combination, on apoptosis, necrosis and autophagy induction in the human cervical carcinoma cell line(HeLa). Imperatorin appeared to be a potent autophagy inducer, rather than a necrotic or apoptotic one. Incontrast, cisplatin induced mainly apoptosis and necrosis after 6 h and 24 h, while longer incubation resultedonly in necrosis induction. When HeLa cells were incubated with both drugs, autophagy appeared most frequently,although to a smaller extent than that observed after imperatorin administered alone. At the molecularlevel, autophagy was correlated with the presence of the cleaved form of microtubule-associated protein 1 lightchain LC3 — LC3II. It was also accompanied by the inhibition of heat shock proteins Hsp27 and Hsp72 expression.Our results indicate that imperatorin alone, or in combination with cisplatin, is mainly an autopahgy inducerin HeLa cells

The use of morphometric and fractal parameters to assess the effects of 5-fluorouracil, interferon and dexamethasone treatment on colonic anastomosis healing: an experimental study in rats

Adjuvant chemotherapy and steroid therapy have been demonstrated to interfere with the wound healing process. The aim of this study was to evaluate the effects of 5-fluorouracil, interferon, and dexamethasone, on the healing of colon anastomosis by assessing morphometric and fractal parameters of the colonic wall. An experimental anastomosis of the ascending colon was performed in 60 male Wistar rats, which were then randomly assigned to four groups. On the second to sixth post-operative days, the rats were administered 5-fluorouracil, interferon-α, dexamethasone, or 0.9% NaCl solution as a control. Macroscopic, histomorphometric and microbiological evaluation was performed in order to assess healing of the anastomosis. In three animals from the dexamethasone group, there was leakage of anastomosis; adhesion formation was highest in the interferon group, and significantly higher than in the control and 5-fluorouracil groups. Histomorphometric parameter alterations were most pronounced on the seventh and fourteenth post-operative days in all treatment groups, with submucosal thickness the most affected parameter. Connective tissue fractal dimension was significantly decreased in those animals treated with interferon and dexamethasone. All three pharmaceutical agents impaired healing of anastomosis, and promoted infection in the anastomosis and skin wound sites. As dexamethasone induced both morphometric and macroscopic alterations, it was considered the most detrimental in this study. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 1, pp. 80–89

Factors influencing surgical treatment of breast cancer in elderly patients

Cel pracy: Czynniki związane z wiekiem, takie jak schorzenia dodatkowe, stan sprawności psychofizycznej i uwarunkowania socjalne, powodują, że u części chorych konieczna jest indywidualizacja leczenia onkologicznego. Celem pracy było dokonanie oceny czynników wpływających na zakres leczenia chirurgicznego u pacjentek z rakiem piersi będących w wieku podeszłym. Materiał i metoda: Badaniem objęto grupę 104 kobiet w wieku 65–93 lat, leczonych z powodu raka piersi w jednym ośrodku, w latach 2015–2016. Dla każdej chorej wypełniono standardowy formularz ewaluacyjny z pominięciem danych osobowych i wieku, zawierający wszystkie dane kliniczne pozwalające na kwalifikację do zabiegu operacyjnego. Na podstawie wypełnionych ankiet pierwotnie leczący zespół lekarski dokonał hipotetycznej kwalifikacji do zabiegu operacyjnego. Kolejno porównano wyniki anonimowej kwalifikacji z dokonaną uprzednio kwalifikacją faktyczną i w oparciu o dokumentację medyczną podjęto próbę zidentyfikowania czynników wpływających na indywidualizację leczenia chirurgicznego. Wyniki: U 34% chorych z grupy badanej nastąpiła modyfikacja faktycznego sposobu leczenia w porównaniu z kwalifikacją anonimową. U 28 pacjentek przeprowadzono zabiegi o większym zakresie, u 7 – mniejszym, 22 kobiety miały wykonany bardziej radykalny zabieg w obrębie gruczołu piersiowego, 10 – w zakresie węzłów chłonnych pachowych. U 5 pacjentek przeprowadzono mniejszy zabieg w piersi, u 1 zaś – w zakresie układu chłonnego. Wnioski: Wpływ na podjętą decyzję miały ogólny stan zdrowia, choroby towarzyszące, osobiste preferencje co do rodzaju terapii i względy anatomiczne operowanych. Wykazano, że wiek metrykalny, wielkość guza i przebyte leczenie neoadiuwantowe nie powodowały rozbieżności między kwalifikacją anonimową i faktyczną.Background: Age-related factors, such as comorbidities, psychophysical status and social conditions, make it necessary to individualize anticancer treatment in some patients. The purpose of the study was to assess factors affecting the extent of surgical treatment in elderly breast cancer patients. Material and methods: The study included 104 women aged 65–93 years, who were treated for breast cancer in a single medical center between 2015 and 2016. A standard evaluation form was completed for each patient, excluding personal data and age, but containing clinical data necessary for the qualification for surgery. Based on the completed questionnaires, hypothetical qualification for surgery was performed by a primary medical team. The results of the anonymous qualification were subsequently compared with the previous, actual qualification, and – based on medical documentation – an attempt was made to identify factors affecting the individualization of surgical treatment. Results: Modification of the actual treatment compared to the anonymous qualification was reported for 34% of patients in the study group. More extensive surgeries were performed in 28, less extensive in 7; more radical breast surgery in 22, and more radical surgery in the area of axillary lymph nodes in 10 patients. Five patients underwent minor breast surgery and one patient – a less extensive surgery of the lymphatic system. Conclusions: Surgical decisions depended on patient’s overall health, comorbidities, anatomical aspects and personal preferences regarding the type of therapy. It was shown that age, tumor size and previous neoadjuvant treatment caused no discrepancies between the anonymous and the actual qualification

ovary during postnatal life

receptors in the spiny mouse (Acomys cahirinus

Quercetin suppresses heat shock-induced nuclear translocation of Hsp72

The effect of quercetin and heat shock on the Hsp72 level and distribution in HeLa cells was studied by Western blotting, indirect immunofluorescence and immunogold electron microscopy. In control cells and after quercetin treatment, Hsp72 was located both in the cytoplasm and in the nucleus in comparable amounts. After hyperthermia, the level of nuclear Hsp72 raised dramatically. Expression of Hsp72 in cytoplasm was also higher but not to such extent as that observed in the nucleus. Preincubation of heated cells with quercetin inhibited strong Hsp72 expression observed after hyperthermia and changed the intracellular Hsp72 distribution. The cytoplasmic level of protein exceeded the nuclear one, especially around the nucleus, where the coat of Hsp72 was noticed. Observations indicating that quercetin was present around and in the nuclear envelope suggested an involvement of this drug in the inhibition of nuclear translocation. Our results indicate that pro-apoptotic activity of quercetin may be correlated not only with the inhibition of Hsp72 expression but also with suppression of its migration to the nucleus

Cell death in HeLa cells upon imperatorin and cisplatin treatment

There is growing evidence that commonly applied chemotherapy regimens can be improved by introducing<br />new, specific, active and low side-effect drugs, or by combining substances to obtain the required clinical<br />effect. The aim of the present study was to investigate the effects of imperatorin and cisplatin, applied separately<br />or in combination, on apoptosis, necrosis and autophagy induction in the human cervical carcinoma cell line<br />(HeLa). Imperatorin appeared to be a potent autophagy inducer, rather than a necrotic or apoptotic one. In<br />contrast, cisplatin induced mainly apoptosis and necrosis after 6 h and 24 h, while longer incubation resulted<br />only in necrosis induction. When HeLa cells were incubated with both drugs, autophagy appeared most frequently,<br />although to a smaller extent than that observed after imperatorin administered alone. At the molecular<br />level, autophagy was correlated with the presence of the cleaved form of microtubule-associated protein 1 light<br />chain LC3 — LC3II. It was also accompanied by the inhibition of heat shock proteins Hsp27 and Hsp72 expression.<br />Our results indicate that imperatorin alone, or in combination with cisplatin, is mainly an autopahgy inducer<br />in HeLa cells.There is growing evidence that commonly applied chemotherapy regimens can be improved by introducing<br />new, specific, active and low side-effect drugs, or by combining substances to obtain the required clinical<br />effect. The aim of the present study was to investigate the effects of imperatorin and cisplatin, applied separately<br />or in combination, on apoptosis, necrosis and autophagy induction in the human cervical carcinoma cell line<br />(HeLa). Imperatorin appeared to be a potent autophagy inducer, rather than a necrotic or apoptotic one. In<br />contrast, cisplatin induced mainly apoptosis and necrosis after 6 h and 24 h, while longer incubation resulted<br />only in necrosis induction. When HeLa cells were incubated with both drugs, autophagy appeared most frequently,<br />although to a smaller extent than that observed after imperatorin administered alone. At the molecular<br />level, autophagy was correlated with the presence of the cleaved form of microtubule-associated protein 1 light<br />chain LC3 — LC3II. It was also accompanied by the inhibition of heat shock proteins Hsp27 and Hsp72 expression.<br />Our results indicate that imperatorin alone, or in combination with cisplatin, is mainly an autopahgy inducer<br />in HeLa cells

Potato fiber protects the small intestinal wall against the toxic influence of acrylamide

Objective: Acrylamide is a neurotoxic, genotoxic substance present in many commonly consumed food products and has been shown to have carcinogenic effects in rodents. The protective effects (if any) of potato fiber preparations, composed of cell wall material from potatoes, against the toxic influence of dietary acrylamide on the small intestinal wall were investigated. Methods: Male mice of the BALB/c strain were used in the study. Acrylamide was administered to the mice in their drinking water (0.5 mg/kg of body weight per day) and one of two types of potato fiber preparations (heated or raw potato fiber preparation) was added to their feed (2% addition to their feed). Histomorphometry of the small intestinal wall, hemoglobin adducts of acrylamide, animal weight, and feed and water consumption analyses were performed. Results: Acrylamide altered the morphology and histology of the small intestinal wall, decreasing proliferation, myenteron and submucosal thicknesses, villus length, fractal dimension, crypt depth, crypt number, and the small intestinal absorptive surface. Conversely, apoptosis, hemoglobin adduct levels, intensity of epithelium staining, enterocyte number, villus epithelial thickness, and crypt width and parameters associated with nerve ganglia were increased. The two potato fiber preparations that were used abolished the negative influences of acrylamide on the small intestinal wall and had no influence on the hemoglobin adduct levels of acrylamide. Conclusion: The negative impact of acrylamide on the histologic structure, regeneration, and innervation of the small intestinal wall and the absorptive function of the small intestinal mucosa can be abolished by dietary potato fiber preparations. (C) 2012 Elsevier Inc. All rights reserved