41 research outputs found
Shape-Induced Terminal Differentiation of Human Epidermal Stem Cells Requires p38 and Is Regulated by Histone Acetylation
Engineered model substrates are powerful tools for examining interactions between stem cells and their microenvironment. Using this approach, we have previously shown that restricted cell adhesion promotes terminal differentiation of human epidermal stem cells via activation of serum response factor (SRF) and transcription of AP-1 genes. Here we investigate the roles of p38 MAPK and histone acetylation. Inhibition of p38 activity impaired SRF transcriptional activity and shape-induced terminal differentiation of human keratinocytes. In addition, inhibiting p38 reduced histone H3 acetylation at the promoters of SRF target genes, FOS and JUNB. Although histone acetylation correlated with SRF transcriptional activity and target gene expression, treatment with the histone de-acetylase inhibitor, trichostatin A (TSA) blocked terminal differentiation on micro-patterned substrates and in suspension. TSA treatment simultaneously maintained expression of LRIG1, TP63, and ITGB1. Therefore, global histone de-acetylation represses stem cell maintenance genes independent of SRF. Our studies establish a novel role for extrinsic physical cues in the regulation of chromatin remodeling, transcription, and differentiation of human epidermal stem cells
Protein Nanosheet Mechanics Controls Cell Adhesion and Expansion on Low-Viscosity Liquids
Adherent
cell culture typically requires cell spreading at the
surface of solid substrates to sustain the formation of stable focal
adhesions and assembly of a contractile cytoskeleton. However, a few
reports have demonstrated that cell culture is possible on liquid
substrates such as silicone and fluorinated oils, even displaying
very low viscosities (0.77 cSt). Such behavior is surprising as low
viscosity liquids are thought to relax much too fast
CCDC 618746: Experimental Crystal Structure Determination
An entry from the Cambridge Structural Database, the worldβs repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures
CCDC 646792: Experimental Crystal Structure Determination
An entry from the Cambridge Structural Database, the worldβs repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures
CCDC 618745: Experimental Crystal Structure Determination
An entry from the Cambridge Structural Database, the worldβs repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures
CCDC 646790: Experimental Crystal Structure Determination
An entry from the Cambridge Structural Database, the worldβs repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures