Smoke-free legislation and child health

In this paper, we aim to present an overview of the scientific literature on the link between smoke-free legislation and early-life health outcomes. Exposure to second-hand smoke is responsible for an estimated 166 ,000 child deaths each year worldwide. To protect people from tobacco smoke, the World Health Organization recommends the implementation of comprehensive smoke-free legislation that prohibits smoking in all public indoor spaces, including workplaces, bars and restaurants. The implementation of such legislation has been found to reduce tobacco smoke exposure, encourage people to quit smoking and improve adult health outcomes. There is an increasing body of evidence that shows that children also experience health benefits after implementation of smoke-free legislation. In addition to protecting children from tobacco smoke in public, the link between smoke-free legislation and improved child health is likely to be mediated via a decline in smoking during pregnancy and reduced exposure in the home environment. Recent studies have found that the implementation of smoke-free legislation is associated with a substantial decrease in the number of perinatal deaths, preterm births and hospital attendance for respiratory tract infections and asthma in children, although such benefits are not found in each study. With over 80% of the world’s population currently unprotected by comprehensive smoke-free laws, protecting (unborn) children from the adverse impact of tobacco smoking and SHS exposure holds great potential to benefit public health and should therefore be a key priority for policymakers and health workers alike

Interactions of Adiponectin and Lipopolysaccharide from Porphyromonas gingivalis on Human Oral Epithelial Cells

BACKGROUND: Periodontitis is an inflammatory disease caused by pathogenic microorganisms, such as Porphyromonas gingivalis, and characterized by the destruction of the periodontium. Obese individuals have an increased risk for periodontitis and show decreased serum levels of adiponectin. This in-vitro study was established to examine whether adiponectin modulates critical effects of lipopolysaccharide (LPS) from P. gingivalis on oral epithelial cells (OECs). METHODOLOGY/PRINCIPAL FINDINGS: The presence of adiponectin and its receptors in human gingival tissue samples and OECs was analyzed by immunohistochemistry and PCR. Furthermore, OECs were treated with LPS and/or adiponectin for up to 72 h, and the gene expression and protein synthesis of pro- and anti-inflammatory mediators, matrix metalloproteinases (MMPs) and growth factors were analyzed by real-time PCR and ELISA. Additionally, cell proliferation, differentiation and in-vitro wound healing were studied. The nuclear translocation of NFκB was investigated by immunofluorescence. Gingival tissue sections showed a strong synthesis of adiponectin and its receptors in the epithelial layer. In cell cultures, LPS induced a significant up-regulation of interleukin (IL) 1β, IL6, IL8, MMP1 and MMP3. Adiponectin abrogated significantly the stimulatory effects of LPS on these molecules. Similarly, adiponectin inhibited significantly the LPS-induced decrease in cell viability and increase in cell proliferation and differentiation. Adiponectin led to a time-dependent induction of the anti-inflammatory mediators IL10 and heme oxygenase 1, and blocked the LPS-stimulated NFκB nuclear translocation. CONCLUSIONS/SIGNIFICANCE: Adiponectin may counteract critical actions of P. gingivalis on oral epithelial cells. Low levels of adiponectin, as observed in obese individuals, may increase the risk for periodontal inflammation and destruction

Acúmulo de nutrientes por três cultivares de alface cultivadas em condições do Semi-Árido Accumulation of nutrients by three lettuce cultivars grown under Semi-arid conditions

O presente trabalho teve como objetivo determinar o acúmulo de nutrientes em cultivares de alface cultivadas em condições do semi-árido. O experimento foi conduzido em área experimental da Universidade Federal Rural do Semi-Árido em solo classificado como Argissolo Vermelho-Amarelo. O delineamento experimental utilizado foi em blocos casualizados completos em esquema fatorial 3 x 5 com quatro repetições. Os tratamentos resultaram da combinação de três cultivares de alface (Babá de Verão, Tainá e Verônica) e cinco épocas de coletas (7, 12, 17, 22 e 27 dias após transplantio - DAT). A máxima produção de matéria seca foi obtida aos 27 DAT, sendo de 8,9; 6,9 e 6,4 g planta-1, respectivamente para as cultivares Babá de Verão, Tainá e Verônica. O período de maior demanda para N, P, K e Mg foi de 22 a 27 DAT e para Ca de 17 a 22 DAT, em todas as cultivares. A ordem decrescente dos nutrientes acumulados pelas cultivares de alface foi: K, N, P, Mg e Ca.<br>The objective of this work was to determine the accumulation of nutrients by lettuce cultivars in conditions of Semi-arid. The experiment was carried in the field of University Federal Rural do Semi-Árido, in Mossoró, Rio Grande do North State, Brazil, in soil Alfissol. The experimental design was of randomized complete blocks, with four replications, in a 3 x 5 factorial scheme, being evaluated the cultivars of lettuce: Babá de Verão, Tainá and Verônica and sampling times 7, 12, 17, 22 and 27 days after transplanting (DAT). The maximum accumulation of dry mass occurred at 27 DAT, being of 8.9; 6.9 and 6.4 g/plant, respectively to cultivars Baby de Verão, Tainá and Verônica. The period of larger demand for N, P, K and Mg occurred from 22 to 27 DAT and for Ca of 17 the 22 DAT in all to cultivars. The nutrients in decreasing order of accumulation by the lettuce cultivars were: K, N, P, Mg e Ca

The Newly Developed CRF1-Receptor Antagonists, NGD 98-2 and NGD 9002, Suppress Acute Stress-Induced Stimulation of Colonic Motor Function and Visceral Hypersensitivity in Rats

Corticotropin releasing factor receptor 1 (CRF(1)) is the key receptor that mediates stress-related body responses. However to date there are no CRF(1) antagonists that have shown clinical efficacy in stress-related diseases. We investigated the inhibitory effects of a new generation, topology 2 selective CRF(1) antagonists, NGD 98-2 and NGD 9002 on exogenous and endogenous CRF-induced stimulation of colonic function and visceral hypersensitivity to colorectal distension (CRD) in conscious rats. CRF(1) antagonists or vehicle were administered orogastrically (og) or subcutaneously (sc) before either intracerebroventricular (icv) or intraperitoneal (ip) injection of CRF (10 µg/kg), exposure to water avoidance stress (WAS, 60 min) or repeated CRD (60 mmHg twice, 10 min on/off at a 30 min interval). Fecal pellet output (FPO), diarrhea and visceromotor responses were monitored. In vehicle (og)-pretreated rats, icv CRF stimulated FPO and induced diarrhea in >50% of rats. NGD 98-2 or NGD 9002 (3, 10 and 30 mg/kg, og) reduced the CRF-induced FPO response with an inhibitory IC(50) of 15.7 and 4.3 mg/kg respectively. At the highest dose, og NGD 98-2 or NGD 9002 blocked icv CRF-induced FPO by 67–87% and decreased WAS-induced-FPO by 23–53%. When administered sc, NGD 98-2 or NGD 9002 (30 mg/kg) inhibited icv and ip CRF-induced-FPO. The antagonists also prevented the development of nociceptive hyper-responsivity to repeated CRD. These data demonstrate that topology 2 CRF(1) antagonists, NGD 98-2 and NGD 9002, administered orally, prevented icv CRF-induced colonic secretomotor stimulation, reduced acute WAS-induced defecation and blocked the induction of visceral sensitization to repeated CRD