Dynamic scaffolds for neuronal signaling: in silico analysis of the TANC protein family.

Fiesta de Tosantos (1101001

An experimental protocol to access immersiveness in video games

In the video game industry, great importance is given to the experience that the user has while playing a game. In particular, this experience benefits from the players' perceived sense of being in the game or immersion. The level of user immersion depends not only on the game's content but also on how the game is displayed, thus on its User Interface (UI) and the Head's-Up Display (HUD). Another factor influencing immersiveness that has been found in the literature is the player's expertise: the more experience the user has with a specific game, the less they need information on the screen to be immersed in the game. Player's level of immersion can be accessed by using both questionnaires of their perceived experience and exploiting their behavioural and physiological responses while playing the target game. Therefore, in this paper, we propose an experimental protocol to access immersiveness of gamers while playing a third-person shooter (Fortnite) with UIs with a standard, a dietetic, and a proposed HUD. A subjective evaluation of the immersion will be provided by completing the Immersive Experience Questionnaire (IEQ), while objective indicators will be provided by face tracking, behaviour and physiological responses analyses. The ultimate goal of this study is to define guidelines for video game UI development that can enhance the players' immersion.Comment: Accepted at the Italian Workshop on Artificial Intelligence for Human Machine Interaction (AIxHMI 2023), November 06, 2023, Rome, Ital

Very slightly anomalous leakage of CO2, CH4 and radon along the main activated faults of the strong L’Aquila earthquake (Magnitude 6.3, Italy). Implications for risk assessment monitoring tools & public acceptance of CO2 and CH4 underground storage.

The 2009-2010 L'Aquila seismic sequence is still slightly occurring along the central Apenninic Belt (August 2010), spanning more than one year period. The main- shock (Mw 6.3) occurred on April 6th at 1:32 (UTC). The earthquake was destructive and caused among 300 casualties. The hypocenter has been located at 42.35°N, 13.38° at a depth of around 10 km. The main shock was preceded by a long seismic sequence starting several months before (i.e., March, 30, 2009 with Mw 4.1; April, 5 with Mw 3.9 and Mw 3.5, a few hours before the main shock). A lot of evidences stress the role of deep fluids porepressure evolution – possibly CO2 or brines - as occurred in the past, along seismically activated segments in Apennines. Our geochemical group started to survey the seismically activated area soon after the main-shock, by sampling around 1000 soil gas points and around 80 groundwater points (springs and wells, sampled on monthly basis still ongoing), to help in understanding the activated fault segments geometry and behaviour, as well as leakage patterns at surface (CO2, CH4, Radon and other geogas as He, H2, N2, H2S, O2, etc...), in the main sector of the activated seismic sequence, not far from a deep natural CO2 reservoir underground (termomethamorphic CO2 from carbonate diagenesis), degassing at surface only over the Cotilia-Canetra area, 20 km NW from the seismically activated area. The work highlighted that geochemical measurements on soils are very powerful to discriminate the activated seismogenic segments at surface, their jointing belt, as well as co-seismic depocenter of deformation. Mostly where the measured “threshold” magnitude of earthquakes (around 6), involve that the superficial effects could be absent or masked, our geochemical method demonstrated to be strategic, and we wish to use these methods in CO2 analogues/CO2 reservoir studies abroad, after done in Weyburn. The highlighted geochemical -slight but clear- anomalies are, in any case, not dangerous for the human health and keep away the fear around the CO2-CH4 bursts or explosions during strong earthquakes, as the L'Aquila one, when these gases are stored naturally/industrially underground in the vicinity (1-2 km deep). These findings are not new for these kind of Italian seismically activated faults and are very useful for the CO2- CH4 geological storage public acceptance: not necessarily (rarely or never) these geogas escape abruptly from underground along strongly activated faults

Imbalance of Mg Homeostasis as a Potential Biomarker in Colon Cancer

Background: Increasing evidences support a correlation between magnesium (Mg) homeostasis and colorectal cancer (CRC). Nevertheless, the role of Mg and its transporters as diagnostic markers in CRC is still a matter of debate. In this study we combined X-ray Fluorescence Microscopy and databases information to investigate the possible correlation between Mg imbalance and CRC. Methods: CRC tissue samples and their non-tumoural counterpart from four patients were collected and analysed for total Mg level and distribution by X-Ray Fluorescence Microscopy. We also reviewed the scientific literature and the main tissue expression databases to collect data on Mg transporters expression in CRC. Results: We found a significantly higher content of total Mg in CRC samples when compared to non-tumoural tissues. Mg distribution was also impaired in CRC. Conversely, we evidenced an uncertain correlation between Mg transporters expression and colon malignancies. Discussion: Although further studies are necessary to determine the correlation between different cancer types and stages, this is the first report proposing the measurement of Mg tissue localisation as a marker in CRC. This study represents thus a proof-of-concept that paves the way for the design of a larger prospective investigation of Mg in CRC

Phenotypic and genetic characterization of a family carrying two Xq21.1-21.3 interstitial deletions associated with syndromic hearing loss

Sensorineural hearing impairment is a common pathological manifestation in patients affected by X-linked intellectual disability. A few cases of interstitial deletions at Xq21 with several different phenotypic characteristics have been described, but to date, a complete molecular characterization of the deletions harboring disease-causing genes is still missing. Thus, the aim of this study is to realize a detailed clinical and molecular analysis of a family affected by syndromic X-linked hearing loss with intellectual disability

Antimeningococcal and antipneumococcal vaccination determinants: A European systematic literature review

EACKGROUND: ESCULAPIO is a multicenter project, funded by the Italian Centre for Disease Prevention and Control, aimed at implementing communication strategies to improve vaccination knowledge and attitudes among different target populations. OBJECTIVE: The objective of the Sicilian research unit was, in the first phase, to identify, through systematic literature revision, which vaccination determinants play a role in the uptake of recommended vaccines included in the Italian Vaccination Plan. DESIGN: A systematic literature review was carried out on studies describing the determinants underlying pneumococcal and meningococcal vaccination uptake. The analysis was limited to papers published in English from 2000 to date. RESULTS: A total of 188 (meningococcal) and 731 (pneumococcal) papers were found. After selection by publication data, country (Europe), article type (original article), target population (healthy subjects), 7 (meningococcal) and 4 ( pneumococcal) manuscripts were finally included in the analysis. For meningococcal vaccination a better socioeconomic status is related to vaccination acceptance, whereas distance from immunization service is a negative determinant. For pneumococcal vaccination the determinants related to vaccination uptake are older parental age and a strong vaccine recommendation. Conversely, when the vaccine needs to be paid for, a refusal is more likely. CONCLUSIONS: Our results show that payment for vaccination is a major barrier and communication about meningococcal and pneumococcal vaccination should be targeted towards specific population groups, especially through the counseling activities by health professionals

Residual neurotoxicity in ovarian cancer patients in clinical remission after first-line chemotherapy with carboplatin and paclitaxel: The Multicenter Italian Trial in Ovarian cancer (MITO-4) retrospective study

BACKGROUND: Carboplatin/paclitaxel is the chemotherapy of choice for advanced ovarian cancer, both in first line and in platinum-sensitive recurrence. Although a significant proportion of patients have some neurotoxicity during treatment, the long-term outcome of chemotherapy-induced neuropathy has been scantly studied. We retrospectively assessed the prevalence of residual neuropathy in a cohort of patients in clinical remission after first-line carboplatin/paclitaxel for advanced ovarian cancer. METHODS: 120 patients have been included in this study (101 participating in a multicentre phase III trial evaluating the efficacy of consolidation treatment with topotecan, and 19 treated at the National Cancer Institute of Naples after the end of the trial). All patients received carboplatin (AUC 5) plus paclitaxel (175 mg/m(2)) every 3 weeks for 6 cycles, completing treatment between 1998 and 2003. Data were collected between May and September 2004. Residual sensory and motor neurotoxicity were coded according to the National Cancer Institute – Common Toxicity Criteria. RESULTS: 55 patients (46%) did not experience any grade of neurological toxicity during chemotherapy and of these none had signs of neuropathy during follow-up. The other 65 patients (54%) had chemotherapy-induced neurotoxicity during treatment and follow-up data are available for 60 of them. Fourteen out of 60 patients (23%) referred residual neuropathy at the most recent follow-up visit, after a median follow up of 18 months (range, 7–58 months): 12 patients had grade 1 and 2 patients grade 2 peripheral sensory neuropathy; 3 patients also had grade 1 motor neuropathy. The remaining 46/60 patients (77%) had no residual neuropathy at the moment of interview: recovery from neurotoxicity had occurred in the first 2 months after the end of chemotherapy in 22 (37%), between 2 and 6 months in 15 (25%), or after more than 6 months in 9 patients (15%). Considering all 120 treated patients, there was a 15% probability of persistent neurological toxicity 6 months after the end of chemotherapy. CONCLUSION: A significant proportion of patients with advanced ovarian cancer treated with first-line carboplatin/paclitaxel suffer long-term residual neuropathy. This issue should be carefully taken into account before considering re-treatment with the same agents in sensitive recurrent disease