Additional postoperative complications.

<p>Additional postoperative complications.</p

Factors associated with postoperative atrial fibrillation.

<p>Factors associated with postoperative atrial fibrillation.</p

G-tube management and diet.

<p>G-tube management and diet.</p

Supplemental Material, Supplemental_Info_for_Scalable_Pipeline_for_HTFC_by_Wilson_et_al – A Scalable Pipeline for High-Throughput Flow Cytometry

<p>Supplemental Material, Supplemental_Info_for_Scalable_Pipeline_for_HTFC_by_Wilson_et_al for A Scalable Pipeline for High-Throughput Flow Cytometry by Aaron C. Wilson, Ioannis K. Moutsatsos, Gary Yu, Javier J. Pineda, Yan Feng, and Douglas S. Auld in SLAS Discovery</p

Study flow chart.

<p>*Recruited via email (n = 609,960) or banner ad (the number of impressions that men were exposed to are not available). †Completed baseline behavioral survey.</p

McNemar’s Post Hoc Tests for Primary Outcomes: Baseline to 60-Day Follow-up Behaviors.

*<p>Proportions in rows may not add to 1.00 due to rounding;</p>†<p>All baseline (Survey 1) and follow-up (Survey 2) chi-square and p-values are paired data, exact McNemar tests;</p>‡<p>Unprotected anal intercourse. The McNemar's odds ratio was calculated by dividing the proportion reporting ‘no to yes’ (from baseline to follow-up) in the numerator over the proportion reporting ‘yes to no’ (from baseline to follow-up) in the denominator. *p≤.05, **p≤.01, ***p≤.001.</p

Sexual Behavior in the 60 Days Prior to Enrollment by Randomization Group.

<p>Some variables have missing data.</p>*<p>Baseline HIV disclosure data were only available for the last encounter in the 60 days prior to baseline. In addition, 13 cases did not report any past 60-day one-on-one sexual encounters and therefore had no data for these questions.</p>†<p>HIV testing among HIV-negative and untested status men.</p>‡<p>Combined male and female partner data for up to the last 3 sexual encounters; 51 cases included female partners.</p>§<p>STI  =  sexually transmitted infections. Both the baseline and follow-up behavioral surveys inquired about any STIs diagnosed by a nurse or physician in the past 60 days, which included chancroid, chlamydia, gonorrhea, herpes, human papillomavirus, lymphogranuloma venereum, nongonococcal urethritis, syphilis, and hepatitis A, B, or C. Significant findings are in bold.</p

A High Content Screen in Macrophages Identifies Small Molecule Modulators of STING-IRF3 and NFkB Signaling

We screened a library of bioactive small molecules for activators and inhibitors of innate immune signaling through IRF3 and NFkB pathways with the goals of advancing pathway understanding and discovering probes for immunology research. We used high content screening to measure the translocation from the cytoplasm to nucleus of IRF3 and NFkB in primary human macrophages; these transcription factors play a critical role in the activation of STING and other pro-inflammatory pathways. Our pathway activator screen yielded a diverse set of hits that promoted nuclear translocation of IRF3 and/or NFkB, but the majority of these compounds did not cause activation of downstream pathways. Screening for antagonists of the STING pathway yielded multiple kinase inhibitors, some of which inhibit kinases not previously known to regulate the activity of this pathway. Structure–activity relationships (SARs) and subsequent chemical proteomics experiments suggested that MAPKAPK5 (PRAK) is a kinase that regulates IRF3 translocation in human macrophages. Our work establishes a high content screening approach for measuring pro-inflammatory pathways in human macrophages and identifies novel ways to inhibit such pathways; among the targets of the screen are several molecules that may merit further development as anti-inflammatory drugs

Primary Outcome Behaviors at 60-Day Follow-up.

<p>All variables have missing data.</p>*<p>HIV disclosure and anal sex variables include sex partner data for up to the 3 last sexual encounters; 1,720 did not report disclosure data due to loss to follow-up (1,461) or drop-out during the follow-up survey (142), no sex during follow-up (104), or only multiple-partner encounter data at baseline and thus no one-on-one encounter data (13).</p>†<p>Unprotected anal intercourse (UAI) was defined as ‘any’ unprotected (without a condom) insertive and/or receptive sex (yes/no).</p>‡<p>HIV Testing among HIV-negative and previously untested status men.</p>§<p>Since baseline, 74 men (5%) reported bacterial and/or newly diagnosed viral sexually transmitted infections (STIs), which included chancroid, chlamydia, gonorrhea, herpes, human papillomavirus, lymphogranuloma venereum, nongonococcal urethritis, syphilis, and hepatitis A, B, or C. Significant findings are in bold.</p

Baseline Demographic and Behavioral Characteristics by Randomization Group.

<p>Overall sample includes participants who reported male partners only (n = 2,950, 95%), male and female partners (n = 113, 4%), and male and transgender partners (n = 16, 1%); 13 participants did not report one-on-one sexual encounters and did not have encounter-specific data.</p