Neutralization ICs of sera collected at various times after infection against the infecting virus

Each panel represents neutralization of macaques infected with the same virus: a) SIVMneCL8; b) SIVMne35wkSU; c) SIVMne170; d) SIVMne027. For each panel, the x axis shows the time when sera samples were collected. The y-axis shows the IC– the reciprocal dilution of sera required to inhibit infection by 50%. Neutralization ICs measured by the TZM-bl cells are shown in solid lines. Neutralization ICs measured in sMAGI cells are shown in dotted lines.<p><b>Copyright information:</b></p><p>Taken from "Heavily glycosylated, highly fit SIVMne variants continue to diversify and undergo selection after transmission to a new host and they elicit early antibody dependent cellular responses but delayed neutralizing antibody responses"</p><p>http://www.virologyj.com/content/5/1/90</p><p>Virology Journal 2008;5():90-90.</p><p>Published online 4 Aug 2008</p><p>PMCID:PMC2518139.</p><p></p

Western blotting analysis of purified plant-derived and CHO-derived HIV-1 env using 2G12.

<p>(lane 1∶89.6P gp140ΔCFI-KDEL (#188), lane 2∶89.6P gp140ΔCFI (#188AH) and lane 3: <b>^BAL</b>CHO gp120. 200 ng of each sample was loaded.</p

Titration of <i>N.benthamiana</i>/p19-derived 2G12 and CHO-derived 2G12 from different days of harvest in the TMZ-bl assay.

<p>Percent neutralization was assessed at various dilutions of CHO-derived 2G12 and purified plant-derived 2G12 extracted from leaves at days 6,12 and 16 post infiltration.</p

Comparison of binding of plant-derived HIV 2G12, b12, 2F5 and VRC01, CHO-derived 2G12, 2F5 and A32 and HEK-produced VRC01 to plant-derived 89.6P gp140ΔCFI-KDEL (#188) by direct ELISA.

<p>Purified plant-derived mAbs produced in SRI or <i>N.benthamiana</i> were harvested at the times indicated.</p

Accumulation of recombinant 2G12-KDEL and 89.6P gp140ΔCFI-KDEL env in leaves using the <i>Nb/</i>p19 transient plant expression system.

<p>Extracts produced from the equivalent of 3 mg (2G12-KDEL) (A) and 4.5 mg 89.6Pgp140ΔCFI env (B) of leaf biomass harvested from days 6–24 post-infiltration were loaded onto the gel and proteins detected by anti-KDEL Western blotting.</p

Comparison of the neutralization titers of <i>N.benthamiana</i>/p19-derived and control mAbs at different harvest times.

<p>IC50s represent the concentration at which relative luminescence units (RLUs) are reduced by 50% versus virus control wells. Times are indicated.</p

Comparison of the neutralization titers of <i>N.tabacum</i>-derived and CHO-derived mAbs using pseudovirus-based TZM-bl cells.

<p>All leaves were harvested at day 4. IC50s represent the concentration at which relative luminescence units (RLUs) are reduced by 50% versus virus control wells.</p

ADCVI activity of plant-derived glycovariants of VRC01 (A), b12 (B) and 2G12 (C) against SF162P3 and the US657 isolates.

<p>Black lines: OGM glycans (KDEL tag), red lines: complex glycoforms (non-KDEL), green lines: HEK-VRC01 and CHO-2G12.</p

Comparison of b12 and 2G12 transudated to the vagina following intravenous administration.

<p>Each antibody treatment group consisted of three female Indian Rhesus macaques which were <i>i.v.</i>-administered 5 mg/kg of either b12 or 2G12 following Depo-provera treatment. Vaginal secretions from each animal were absorbed to cellulose wicks. A set of 3 samples per animal was taken at 6 hours, 12 hours, 24 hours, 4 days, and 7 days post <i>i.v.</i> antibody administration. The concentration of antibody in mucosal secretions was determined by ELISA from the clarified supernatant extracted from the wicks. Resulting data were compared to the corresponding antibody standard curve using nonlinear regression. Arithmetic means and standard deviations were calculated for each set of triplicate samples per animal. Data points were calculated from all animals at each timepoint and error bars represent the standard error of means. The typical time for viral challenge in protection experiments is indicated. The differences in the mean concentrations of b12 and 2G12 at each timepoint were evaluated in a student's t test and determined to be non-significant. Analyses performed in GraphPad Prism Software for Mac, Version 5.0a.</p

Numerical results from the SPR analysis of HIV-BAL env binding.

1<p>As reported <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058724#pone.0058724-Floss1" target="_blank">[38]</a>, the comparison to these measurements shows an excellent reproducibility, with marginal differences being within experimental limits.</p>2<p>N/A. = not applicable.</p