Summary of MOI and <i>F_WS</i> estimates in Burkina Faso, Mali and the combined (West Africa) population.

<p>Summary of MOI and <i>F_WS</i> estimates in Burkina Faso, Mali and the combined (West Africa) population.</p

Additional file 7: Table S4. of Association mapping by pooled sequencing identifies TOLL 11 as a protective factor against Plasmodium falciparum in Anopheles gambiae

Primers for gene silencing and efficiency verification. (XLSX 10 kb

Additional file 4: Figure S3. of Association mapping by pooled sequencing identifies TOLL 11 as a protective factor against Plasmodium falciparum in Anopheles gambiae

Manhattan plot for colony Fd03. Results are given for the two replicates individually and the values combined by Fisher’s method. All genotyped loci were tested for association with two phenotypes by logistic regression using PLINK. Infection prevalence (blue) was defined as having more than one oocyst in the dissected midgut, infection intensity (red, measured only for mosquitoes carrying ≥1 oocyst) as having >5 oocysts. Dashed line represents a 0.01 p-value (i.e., 1/p = 100). Genes in the regions are shown below; Toll11 and Toll10 are the two rightmost genes on the positive strand. The combined plot represents values from reps 1 and 2 combined by the method of Fisher [47]. (PDF 127 kb

Additional file 2: Figure S2. of Association mapping by pooled sequencing identifies TOLL 11 as a protective factor against Plasmodium falciparum in Anopheles gambiae

Genome wide total heterozygosity and relative heterozygosity for founder colony Fd09. Plots depict heterozygosity measures for colony Fd09 across all chromosomes. Total pooled heterozygosity (Hp) was calculated in a sliding 10 kb window along the chromosome within the Fd09 colony. Dots indicate minimum and maximum values for a 1 Mb window, the black line indicates the average heterozygosity and the gray shading represents the standard deviation of total pooled heterozygosity across a 1 Mb window. Relative diversity (HpR) per 1 Mb window, calculated as the proportion of heterozygosity in a given pool relative to total heterozygosity within the source Fd09 colony. Color of point indicates per window elevated heterozygosity (blue), or reduced heterozygosity (red), plotted as log base 10 of the relative diversity. Phenotype pool identity is indicated in the lower left of each panel (high, low, zero oocysts). A relative heterozygosity value of 1 indicates the same heterozygosity levels in tested pool as compared to all other pools, values greater than 1 indicate greater heterozygosity in the tested pool and values less than 1 indicate lower heterozygosity in the tested pool. Given the log scale values of 0.5 and 2.0 are equidistant from 1. Candidate loci 9.1 and 9.2 are indicated by dark blue and light blue shading, respectively, on Chromosome 2. At locus 9.1, relative heterozygosity is decreased in the high pool and simultaneously reduced in the low and zero pools, while at locus 9.2, relative heterozygosity in increased in the high pool and decreased in the other pools. Each pool was comprised of DNA from 20 individual mosquitoes. (PDF 4973 kb

<i>F_WS</i> against MSP1 MOI, MSP2 MOI, and maximum MOI.

<p><i>F_WS</i> against MSP1 MOI, MSP2 MOI, and maximum MOI.</p

Additional file 3: Table S1. of Association mapping by pooled sequencing identifies TOLL 11 as a protective factor against Plasmodium falciparum in Anopheles gambiae

Fd03 Infection Prevalence Association and Table S2. Fd03 Infection Intensity Association (XLSX 15 kb

Additional file 5: Table S3. of Association mapping by pooled sequencing identifies TOLL 11 as a protective factor against Plasmodium falciparum in Anopheles gambiae

All predicted genes within the mapped locus, Pfin 7. (XLSX 13 kb

Additional file 1: Figure S1. of Association mapping by pooled sequencing identifies TOLL 11 as a protective factor against Plasmodium falciparum in Anopheles gambiae

Genome wide total heterozygosity and relative heterozygosity for founder colony Fd03. Plots depict heterozygosity measures for colony Fd03 across all chromosomes. Total pooled heterozygosity (Hp) was calculated in a sliding 10 kb window along the chromosome within the Fd03 colony. Dots indicate minimum and maximum values for a 1 Mb window, the black line indicates the average heterozygosity and the gray shading represents the standard deviation of total pooled heterozygosity across a 1 Mb window. Relative diversity (HpR) per 1 Mb window, calculated as the proportion of heterozygosity in a given pool relative to total heterozygosity within the source Fd03 colony. Color of point indicates per window elevated heterozygosity (blue), or reduced heterozygosity (red), plotted as log base 10 of the relative diversity. Phenotype pool identity is indicated in the lower left of each panel (high, low, zero oocysts). A relative heterozygosity value of 1 indicates the same heterozygosity levels in tested pool as compared to all other pools, values greater than 1 indicate greater heterozygosity in the tested pool and values less than 1 indicate lower heterozygosity in the tested pool. Given the log scale values of 0.5 and 2.0 are equidistant from 1. Candidate locus 3.1 is indicated by the red vertical shaded bar at coordinates 17.4-19.1 Mb. In this interval, relative heterozygosity is increased in the high pool and simultaneously reduced in the low and zero pools. Pools were comprised of DNA from 20 (zero oocyst pool), 17 (low oocyst pool), or 14 (high oocyst pool) individuals. (PDF 4909 kb

Summary of sequencing coverage per lane across 59 clinical samples.

<p>Data is averaged across all sample lanes within the given ranges of human DNA quantities. On average, each sample was sequenced on 2 lanes.</p

Square root of average coverage per base against human DNA proportion for 59 sequenced clinical samples.

<p>Sequence read lengths were either 54 bp (grey spots) or 76 bp (black spots). Each dot represents a sample. Where a sample was sequenced on more than one lane, the average sequence coverage is presented. The median (Square root of average coverage) intra-sample standard deviation was 0.91, and inter-quartile range was 0.70–1.35.</p