Biallelic and Genome Wide Association Mapping of Germanium Tolerant Loci in Rice (Oryza sativa L.)

Funding: This project was partially funded by a Biotechnology and Biological Sciences Research Council (BBSRC) grant (BB/J003336/1) awarded to AHP. The work was also supported by a self-funded studentship (PT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

Using a VLE to enhance a Foundation Chemistry laboratory module

For the past few years, we have been experimenting with an e-learning approach to our introductory laboratory classes for first year students. Our overall objective was to maximise students’ useful time in the laboratory. We considered that time spent with students gathered around a desk watching a demonstration is not an efficient use of staff or students’ time.It is well recognised that students’ performance in the laboratory can be enhanced if they are familiar with the background of the experiments which will be conducted, hence the use of ‘pre-labs’. We have been delivering our ‘pre-labs’ electronically by requiring students to work through a package before coming to the laboratory. As well as covering the theory and background to the experiment, short video clips have been included so that students will also have seen the experiment being performed. They should at least recognise the apparatus! The package concludes with a short assessment quiz which must be completed.The packages were mounted on the University network using WebCT and meant that students could undertake the exercises at a time (and place) of their choosing rather than being confined to set laboratory hours.This communication will describe the packages and our experiences as well as an initial evaluation of our approach. Although largely anecdotal, staff felt that they spent less time on more mundane aspects of laboratory work and more time discussing chemistry.Students also felt that they were better prepared for the experiments before they came to the laboratory. Some of the pitfalls and technical problems that had to be overcome willalso be described

Genome-Wide Association Mapping for Salt Tolerance of Rice Seedlings Grown in Hydroponic and Soil Systems Using the Bengal and Assam Aus Panel

Data Availability Statement The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation, to any qualified researcher. Funding The production of the BAAP was conducted under BBSRC funded project BB/J003336/1, with minor development under BB/N013492/1. CC gratefully acknowledges financial support from China Scholarship Council. Acknowledgments We thank Yang Guo, Wenchuang He, and Aadil Tantray for providing help measuring the phenotypic traits and editing the manuscript.Peer reviewedPublisher PD

Sonochemical Modification of Carbon Nanotubes for Enhanced Nanocomposite Performance

Multi-walled carbon nanotubes (CNTs) have been treated using 20 kHz ultrasound in combination with dilute nitric and sulfuric acids at much lower concentrations than previously reported. The measurements revealed an optimum set of sonication conditions (in this case 30 min at 12 W cm−2) exists to overcome aggregation of the nanotubes and to allow efficient dispersion in ethanol or in chitosan. Transmission electron microscopy and Raman spectroscopy suggested the removal of amorphous material and reduction of the CNT diameter as well as modifications to their defect structures. The surface oxidation was determined by FTIR spectroscopy. At longer times or higher ultrasound intensities, degradation such as nanotube shortening and additional defect generation in the graphitic network occurred and the benefits of using ultrasound decreased. The modified CNTs were used as fillers for chitosan films and gave a tenfold increase in tensile strength and integrity of the films. The methodology was combined with sonochemical generation of gold or iron oxide nanoparticles to produce a range of functional membranes for catalytic reductive hydrogenation or dye degradation under conditions that are more environmentally benign than those previously used. Our results further add to the usefulness of sonochemistry as a valuable tool in preparative materials chemistry but also illustrate the crucial importance of careful control over the experimental conditions if optimum results are to be obtained

Surrogate-driven respiratory motion model for projection-resolved motion estimation and motion compensated Cone-Beam CT reconstruction from unsorted projection data

OBJECTIVE: As the most common solution to motion artefact for Cone-Beam CT (CBCT) in radiotherapy, 4DCBCT suffers from long acquisition time and phase sorting error. This issue could be addressed if the motion at each projection could be known, which is a severely ill-posed problem. This study aims to obtain the motion at each time point and motion-free image simultaneously from unsorted projection data of a standard 3DCBCT scan. 
Approach: Respiration surrogate signals were extracted by the Intensity Analysis method. A general framework was then deployed to fit a surrogate-driven motion model that characterized the relation between the motion and surrogate signals at each time point. Motion model fitting and motion compensated reconstruction were alternatively and iteratively performed. Stochastic subset gradient based method was used to significantly reduce the computation time. The performance of our method was comprehensively evaluated through digital phantom simulation and also validated on clinical scans from six patients.
Results: For digital phantom experiments, motion models fitted with ground-truth or extracted surrogate signals both achieved a much lower motion estimation error and higher image quality, compared with non motion-compensated results.For the public SPARE Challenge datasets, more clear lung tissues and less blurry diaphragm could be seen in the motion compensated reconstruction, comparable to the benchmark 4DCBCT images but with a higher temporal resolution. Similar results were observed for two real clinical 3DCBCT scans. SIGNIFICANCE: This study demonstrated the feasibility of our proposed framework for simultaneous motion model fitting and motion compensated reconstruction using unsorted 3D CBCT projection data