Protective Immunity Induced By A Trypanosoma Cruzi Soluble Extract Antigen In Experimental Chagas' Disease. Role Of Interferon γ

CBA/J mice can be protected against lethal infection with Trypanosoma cruzi by treatment using T. cruzi soluble extract antigen (TCSE). In vivo administration of TCSE (400 μg/mouse) into naive mice increased the cellular proliferative response to Con A and elevated the levels of IFN-γ. The production of IFN-γ was extremely important in controlling the replication of the parasite since the protective activity of TCSE was completely abrogated by in vivo treatment with an anti IFN-γ neutralizing antibody. These results suggest that depending on the level, cytokine production results in the control of replication of the parasite in experimental Chagas' disease.291112Kierszenbaum, F., Cuna, W.R., Beltz, L.A., Sztein, M.B., (1990) J. Immunol., 143, pp. 275-279Kierszenbau, F., Sztein, M.B., Beltz, L.A., (1989) Immunology Today, 10, pp. 129-131Cunningham, D.S., Kuhn, R.E., (1980) J. Parasitol., 66, pp. 16-27Harel-Bellan, A., Joskowicz, M., Dradelizi, D., Eisen, H., (1983) Proc. Natl. Acad. Science USA, 80, pp. 3466-3469Silva, J.S., Twardzik, D.R., Reed, S.G., (1991) J. Exp. Med., 174, pp. 539-545Bermudez, L.E., (1993) J. Immunol., 150, pp. 1838-1845Reed, S.G., Phil, D.L., Grabstein, K.H., (1989) J. Immunol., 142, pp. 2067-2071Plata, F., Wietzerbin, J., Pons, F.G., Falcoff, E., Eisen, H., (1984) Eur. J. Immunol, 14, pp. 930-935Reed, S.G., (1988) J. Immunol., 140, pp. 4342-4347Torrico, F., Heremans, H., Rivera, M.T., Marck, E.V., Billiau, A., Carlier, Y., (1991) J. Immunol., 146, pp. 3626-3632Al-Sabbagh, A., Garcia, C.A.A.C., Diaz-Bardales, B.M., Zaccarias, C., Sakurada, J.K., Santos, L.M.B., (1998) Exp. Parasitol., 89, pp. 304-311Minoprio, P.M., Eisen, H., Forni, L., D'Imperio Lima, M.R., Joskowics, M., Coutinho, A., (1986) J. Immunol., 24, pp. 661-668Tamashiro, W.M.S.C., Repka, D., Sakurada, J.K., Camargo, I.J.B., Araujo, P.M.F., Atta, A.M., Rangel, H.A., (1983) Z. Parasitenkd., 69, pp. 425-434Scott, M.T., Snary, D., (1979) Nature, 282, pp. 73-74Segura, E.L., Paulone, I., Cerisola, J., Gonzalez-Capra, S.M., (1976) J. Parasitol., 62, pp. 131-133Wirth, J.J., Kiersenbaum, F., Sonnenfeld, G., Zlotnik, A., (1985) Infection and Immunity, 49, pp. 61-66Taibi, A., Espinoza, A.G., Ouaissi, A., (1995) Immunol. Letters, 48, pp. 193-200Scott, P., Natovitz, P., Coffman, R.L., Pearce, E., Sher, A., (1988) J. Exp. Med., 168, pp. 1675-168