Evaluation of the weight loss of raw beef cuts vacuum-packaged with two different techniques

In the present study, 25 cuts of shank form adult cattle coming from the same slaughtering batch, were withdrawn just after manual sectioning/deboning, and each divided into two pieces (Prox and Dist) of approximately the same weight, that were vacuum packaged by using two different packaging systems: vacuum chamber machine with a bag material and a thermo-forming packaging machine with top and bottom webs named BAG and THF respectively. The packed cuts were stored at 2-3\ub0C for 20 days. The drip loss was calculated at the end of the storage as the difference between drained weight and net. Internal muscle pH and pH of the exudate present in the package and microbiological analyses (by pooling the samples) were performed at T0 and at the end of the storage. The drip loss, was significantly lower with BAG packaging: this difference was evident after 20 days of storage (average \ub1 STD BAG vs THF = 1.04\ub10.36% vs 1.71\ub10.42%; P<0.01). The values were, in general, low for both the packaging systems, never above 2%. Moreover, shrink bag packages are characterized by better overall pack appearance and less plastic weight per pack. Forming step reduce the thickness of ther-moforming material lowering the mechanical resistance and the barrier to oxygen, on the contrary after shrinking bag materials are thickened. The pH of muscles was stable, although a slight increase was evidenced after 20 (average \ub1 STD BAG vs THF= 5.73\ub10.05 vs 5.78\ub10.09; P<0.01), due to the ageing of meat. The pH of the exudate was equal at T20 (average \ub1 STD BAG vs THF = 5.34\ub10.20 vs 5.33\ub10.17). The drip loss didn\u2019t influence the development of all the microflora; in particular LAB, that represented the main microbial population, showed a gradual increase from T0 (2.20\ub10.41 Log CFU/g) to T20 (average \ub1 STD BAG vs THF= 4.76\ub10.29 Log CFU/g vs 4.75\ub10.0.15 Log CFU/g). No Enterobacteriaceae showed an increase, if compared to the initial counts, due to the prolonged storage and the gradual growth of ephemeral microorganisms, without differences among the two series (Enterobacteriaceae: T0=<1.7 Log CFU/g to T20 average \ub1 STD BAG vs THF = 2.83\ub10.77 Log CFU/g vs 3.09\ub10.0.70 Log CFU/g). In conclusion, the use of the BAG system demonstrated to have an effect in reducing the drip loss of beef cuts during the refrigerated storage, with only slight influence on the other characteristics of raw meat

The stone roof of the tholos of Athena Pronaia in Delphi: structural hypotheses starting from fragments of marble tiles

This contribution is the first step of a multi-disciplinary research project, aimed at studying the roof of the tholos of Athena Pronaia in Delphi, dating back to the first decades of the 4th century BC. The starting point of this research is the fascination exerted by the tectonic quality of the temple, comprising some complex fragments of marble tiles belonging to the roof of the tholos. Despite the number of studies on this sanctuary, the poor state of preservation and lack of original material did not allow archaeologists to perform concluding research on its original configuration, in particular with reference to the reconstruction of the roof. Aware of the complexity of this topic, this contribution aims at exploring two possibilities, namely, on the one hand, that the fragments of tiles constitute elements supported by a wooden structure, and on the other hand, that they are structural elements of a stone system subjected to compressive stresses. Our contribution intends to serve as food for thought on the need for archaeological studies to be accompanied not only by advanced surveys in the field, aimed at the precise definition of the geometry of the finds, but also by architectural and structural investigations which make it possible to verify the feasibility of the hypothetical reconstructions of the architectural elements aware of the construction techniques used in the past

Benign hereditary chorea and deletions outside NKX2-1 : what's the role of MBIP?

Heterozygous point mutations or deletions of the NKX2-1 gene cause benign hereditary chorea (BHC) or a various combinations of primary hypothyroidism, respiratory distress and neurological disorders. Deletions proximal to, but not encompassing, NKX2-1 have been described in few subjects with brain-lung-thyroid syndrome. We report on a three-generation Italian family, with 6 subjects presenting BHC and harboring a genomic deletion adjacent to NKX2-1 and including the gene MBIP, recently proposed to be relevant for the pathogenesis of brain-lung-thyroid syndrome. We observed a clear reduction of NKX2-1 transcript levels in fibroblasts from our patients compared to controls; this finding suggests that MBIP deletion affects NKX2-1 expression, mimicking haploinsufficiency caused by classical NKX2-1 related mutations

Mitochondrial Complex III Deficiency Caused by TTC19 Defects : Report of a Novel Mutation and Review of Literature

We report about a patient with infantile-onset neurodegenerative disease associated with isolated mitochondrial respiratory chain complex III (cIII) deficiency. The boy, now 13 years old, presented with language regression and ataxia at 4 years of age and then showed a progressive course resulting in the loss of autonomous gait and speaking during the following 2 years. Brain MRI disclosed bilateral striatal necrosis. Sequencing of a panel containing nuclear genes associated with cIII deficiency revealed a previously undescribed homozygous rearrangement (c.782_786delinsGAAAAG) in TTC19 gene, which results in a frameshift with premature termination (p.Glu261Glyfs(*)8). TTC19 protein was absent in patient's fibroblasts. TTC19 encodes tetratricopeptide 19, a putative assembly factor for cIII. To date TTC19 mutations have been reported only in few cases, invariably associated with cIII deficiency, but presenting heterogeneous clinical phenotypes. We reviewed the genetic, biochemical, clinical and neuroradiological features of TTC19 mutant patients described to date

Hypertension and sudden death disparate effects of calcium entry blocker and diuretic therapy on cardiac dysrhythmias

This study was designed to evaluate the impact of antihypertensive therapy on cardiac dysrhythmias in 13 hypertensive patients who received calcium entry blockers and in 10 hypertensive patients who received hydrochlorothiazide. Mean arterial pressure fell to a similar extent in both treatment groups; however, left ventricular mass index decreased (from 102±4 to 95±2 g/m2) only in patients receiving calcium entry blockers, but not in those taking hydrochlorothiazide. The prevalence of premature ventricular contractions decreased 74% from 21 14/h to 5.7 ± 6/h in the calcium entry blocker group, but did not change in the hydrochlorothiazide group (15± 17/h to 16± 13/h). Couplets, multiform contractions, ventricular tachycardia, and supraventricular tachycardia were completely abolished after calcium entry blocker therapy, whereas the prevalence of these arrhythmias remained unchanged during treatment with hydrochlorothiazide. We conclude that antihypertensive therapy with calcium entry blockers (but not with thiazide diuretics) reduces left ventricular mass and the prevalence and severity of ventricular dysrhythmias. Whether this reduction will improve the ominous prognosis of left ventricular hypertrophy and diminish the risk of sudden death remains unknown

Higgs particle detection using jets

We study the possibility of detecting the Higgs boson in the intermediate mass range via its two jet channel. We consider only Higgs bosons produced in association with a $t \bar{t}$ pair. Both $t$ and $\bar{t}$ are required to decay semileptonically to reduce the QCD background. The signal is compared with the main background, $t \bar{t} + 2$ jets, after appropriate cuts. A sizable signal above background is seen in our simulation at the parton level. Use of the $t\bar{t}Z$ channel with $Z$ decaying to $l^+ l^-$ is suggested for eliminating theoretical uncertainties in determining the $t \bar{t}H$ signal.Comment: 10 pages, Fig.1 a,b,c,d(surve on request), plain tex, PVAM-HEP-93-

The Low Frequency Instrument in the ESA Planck mission

Measurements of the cosmic microwave background (CMB) allow high precision observation of the cosmic plasma at redshift z~1100. After the success of the NASA satellite COBE, that in 1992 provided the first detection of the CMB anisotropy, results from many ground-based and balloon-borne experiments have showed a remarkable consistency between different results and provided quantitative estimates of fundamental cosmological properties. During the current year the team of the NASA WMAP satellite has released the first improved full-sky maps of the CMB since COBE, leading to a deeper insight in the origin and evolution of the Universe. The ESA satellite Planck, scheduled for launch in 2007, is designed to provide the ultimate measurement of the CMB temperature anisotropy over the full sky, with an accuracy that will be limited only by astrophysical foregrounds, and robust detection of polarisation anisotropy. Planck will observe the sky with two instruments over a wide spectral band (the Low Frequency Instrument, based on coherent radiometers, from 30 to 70 GHz and the High Frequency Instrument, based on bolometric detectors, from 100 to 857 GHz). The mission performances will improve dramatically the scientific return compared to WMAP. Furthermore the LFI radiometers (as well as some of the HFI bolometers) are intrinsically sensitive to polarisation so that by combining the data from different receivers it will be possible to measure accurately the E mode and to detect the B mode of the polarisation power spectrum. Planck sensitivity will offer also the possibility to detect the non-Gaussianities imprinted in the CMB.Comment: 4 pages, 2 figures, to appear in "Proc of International Symposium on Plasmas in the Laboratory and in the Universe: new insights and new challenges", September 16-19, 2003, Como, Ital

A plant natriuretic peptide-like molecule of the pathogen Xanthomonas axonopodis pv. citri causes rapid changes in the proteome of its citrus host

<p>Abstract</p> <p>Background</p> <p>Plant natriuretic peptides (PNPs) belong to a novel class of peptidic signaling molecules that share some structural similarity to the N-terminal domain of expansins and affect physiological processes such as water and ion homeostasis at nano-molar concentrations. The citrus pathogen Xanthomonas axonopodis pv. citri possesses a PNP-like peptide (XacPNP) uniquely present in this bacteria. Previously we observed that the expression of <it>XacPNP </it>is induced upon infection and that lesions produced in leaves infected with a XacPNP deletion mutant were more necrotic and lead to earlier bacterial cell death, suggesting that the plant-like bacterial PNP enables the plant pathogen to modify host responses in order to create conditions favorable to its own survival.</p> <p>Results</p> <p>Here we measured chlorophyll fluorescence parameters and water potential of citrus leaves infiltrated with recombinant purified XacPNP and demonstrate that the peptide improves the physiological conditions of the tissue. Importantly, the proteomic analysis revealed that these responses are mirrored by rapid changes in the host proteome that include the up-regulation of Rubisco activase, ATP synthase CF1 α subunit, maturase K, and α- and β-tubulin.</p> <p>Conclusions</p> <p>We demonstrate that XacPNP induces changes in host photosynthesis at the level of protein expression and in photosynthetic efficiency in particular. Our findings suggest that the biotrophic pathogen can use the plant-like hormone to modulate the host cellular environment and in particular host metabolism and that such modulations weaken host defence.</p