Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by white rot-fungus Pseudotrametes gibbosa isolated from the boreal forest in Northeast China

This study compared laccase production and the degradation of polycyclic aromatic hydrocarbons (PAHs) by  aboriginal white rot-fungus Pseudotrametes gibbosa (found in the northeast forest area of China) and Pleurotus ostreatus (which has been studied both domestically in China and overseas). The results showed that the laccase activity of P. gibbosa was 2841.3 U/l, which was 6 times more than that of P. ostreatus under the same culture conditions. The degradation of Anthracene and pyrene induced by P. gibbosa were 43.43 and 24.26%, while the removal efficiencies induced by P. ostreatus were only 30.12 and 18.76%. The results also showed a positive correlation between the PAHs degradation and laccase activity, and Pseudotrametes gibbosa had significant potential due to its higher laccase production and more potent degradation of PAHs. This study provides technical support for pollution amelioration using aboriginal white-rot fungus.Key words: White-rot fungus, laccase, polycyclic aromatic hydrocarbons, degradation

Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies

Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first ExM method did not result in the retention of native proteins in the gel and relied on custom-made reagents that are not widely available. Here we describe protein retention ExM (proExM), a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins. We validated and demonstrated the utility of proExM for multicolor super-resolution (~70 nm) imaging of cells and mammalian tissues on conventional microscopes.United States. National Institutes of Health (1R01GM104948)United States. National Institutes of Health (1DP1NS087724)United States. National Institutes of Health ( NIH 1R01EY023173)United States. National Institutes of Health (1U01MH106011

A TNF-JNK-Axl-ERK signaling axis mediates primary resistance to EGFR inhibition in glioblastoma.

Aberrant epidermal growth factor receptor (EGFR) signaling is widespread in cancer, making the EGFR an important target for therapy. EGFR gene amplification and mutation are common in glioblastoma (GBM), but EGFR inhibition has not been effective in treating this tumor. Here we propose that primary resistance to EGFR inhibition in glioma cells results from a rapid compensatory response to EGFR inhibition that mediates cell survival. We show that in glioma cells expressing either EGFR wild type or the mutant EGFRvIII, EGFR inhibition triggers a rapid adaptive response driven by increased tumor necrosis factor (TNF) secretion, which leads to activation in turn of c-Jun N-terminal kinase (JNK), the Axl receptor tyrosine kinase and extracellular signal-regulated kinases (ERK). Inhibition of this adaptive axis at multiple nodes rendered glioma cells with primary resistance sensitive to EGFR inhibition. Our findings provide a possible explanation for the failures of anti-EGFR therapy in GBM and suggest a new approach to the treatment of EGFR-expressing GBM using a combination of EGFR and TNF inhibition

High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product

Nannochloropsis are model species for investigating biofuel production by algae. To develop them into an integrated photons-to-fuel production platform, high efficiency transformation methods are necessary. Here, we obtained the beta-tubulin promoter regions of all recognized species of genus Nannochloropsis, and successfully transformed all five marine species by electroporation. In addition, the PCR amplified double stranded DNA fragments (PCR fragments) based transformation system was established in these Nannochloropsis species, which showed much higher transformation efficiency (10.7-61.2 x 10 (6), 1.5-13-fold) than that of linearized plasmid based transformation. The cotransformation of N. salina using a circular plasmid containing a non-selectable GUS gene and a PCR fragment containing only a selection marker cassette was also achieved and found to be very efficient (over 50%). This simple and highly efficient transformation protocol reported in our study provided a useful tool for gene functional analysis and genetic engineering of the oleaginous Nannochloropsis species

Modeling Ecohydrological Processes and Spatial Patterns in the Upper Heihe Basin in China

The Heihe River is the second largest inland basin in China; runoff in the upper reach greatly affects the socio-economic development in the downstream area. The relationship between spatial vegetation patterns and catchment hydrological processes in the upper Heihe basin has remained unclear to date. In this study, a distributed ecohydrological model is developed to simulate the hydrological processes with vegetation dynamics in the upper Heihe basin. The model is validated by hydrological observations at three locations and soil moisture observations at a watershed scale. Based on the simulated results, the basin water balance characteristics and their relationship with the vegetation patterns are analyzed. The mean annual precipitation and runoff increase with the elevation in a similar pattern. Spatial patterns of the actual evapotranspiration is mainly controlled by the precipitation and air temperature. At the same time, vegetation distribution enhances the spatial variability of the actual evapotranspiration. The highest actual evapotranspiration is around elevations of 3000–3600 m, where shrub and alpine meadow are the two dominant vegetation types. The results show the mutual interaction between vegetation dynamics and hydrological processes. Alpine sparse vegetation and alpine meadow dominate the high-altitude regions, which contribute most to the river runoff, and forests and shrub contribute relatively small amounts of water yield

Nitrogen Removal from Mature Landfill Leachate via Anammox Based Processes: A Review

Mature landfill leachate is a complex and highly polluted effluent with a large amount of ammonia nitrogen, toxic components and low biodegradability. Its COD/N and BOD5/COD ratios are low, which is not suitable for traditional nitrification and denitrification processes. Anaerobic ammonia oxidation (anammox) is an innovative biological denitrification process, relying on anammox bacteria to form stable biofilms or granules. It has been extensively used in nitrogen removal of mature landfill leachate due to its high efficiency, low cost and sludge yield. This paper reviewed recent advances of anammox based processes for mature landfill leachate treatment. The state of the art anammox process for mature landfill leachate is systematically described, mainly including partial nitrification–anammox, partial nitrification–anammox coupled denitrification. At the same time, the microbiological analysis of the process operation was given. Anaerobic ammonium oxidation (anammox) has the merit of saving the carbon source and aeration energy, while its practical application is mainly limited by an unstable influent condition, operational control and seasonal temperature variation. To improve process efficiency, it is suggested to develop some novel denitrification processes coupled with anammox to reduce the inhibition of anammox bacteria by mature landfill leachate, and to find cheap new carbon sources (methane, waste fruits) to improve the biological denitrification efficiency of the anammox system

Relationship between Biomass and Biodiversity of Degraded Grassland in the Sanjiangyuan Region of Qinghai–Tibet Plateau

Understanding the mechanisms of diversity–productivity relationships is a central question in community ecology. Grazing is the main driving force affecting biodiversity, function, and stability of grassland ecosystems, and thus should play an important role in mediating diversity-productivity relationships. In this study, we examined the effect of grazing intensity on both aboveground biomass and biodiversity and explored the relationship between them in alpine meadow ecosystems in Sanjiangyuan, which is the source of the Yangtze, Yellow, and Lancang rivers. The results showed that the aboveground biomass and species richness decreased significantly due to multi-state succession in alpine meadows caused by long-term grazing, while the Shannon–Wiener index and Pielou evenness index decreased and then increased with increasing grazing intensity. The relationship between the aboveground biomass and biodiversity was U-shaped. Our results highlighted the opposite pattern of the diversity–productivity relationship under low and medium grazing intensity versus an extremely high grazing intensity; evenness contributed largely to this pattern. This study provided a new perspective on grassland management and the relationship between productivity and biodiversity. Attention should be paid to rational grazing to restore biodiversity and ecosystem functions and services in alpine meadows

Optimizing nitrifying community and achieving partial nitrification with real-time control

A pilot-plant sequencing batch reactor (SBR) with a working volume of 54 m was used in the experiment on biological nitrogen removal via nitrite from municipal wastewater with low COD/N ratio for saving energy and carbon sources. At normal water temperature, the feasibility of achieving and stabilizing nitrogen removal via nitrite from municipal wastewater with the low COD/N ratio (average 2.16) was investigated. The obtained resulted showed that the total nitrogen (TN) in the effluent was less than 3 mg/L and the advanced nitrogen removal was obtained through application of step-feed operation. Moreover, by using the real-time control strategies, the nitrifying communities were optimized. Thus, under normal dissolved oxygen (DO) condition, nitrogen removal via nitrite was successfully achieved at the range of temperature between 12°C to 26°C,. Nitrogen removal via nitrite kept the long-term stability (180 days) and the average NO-N/NOx-N ratio was above 95%. The fluorescence in situ hybridization (FISH) was performed to analyze the quantitative changes of nitrifying microbial communities in the activated sludge. The results also indicated that ammonia-oxidizing bacteria (AOB) became the dominant nitrifying bacteria and nitrite-oxidizing bacteria (NOB) had been washed out of the activated sludge. It indicated that the nitrifying microbial communities were optimized