Efecto del extracto crudo del mesocarpio de Persea americana MILL var. fuerte en hiperlipidemia inducida en Oryctolagus cunniculus

Presently study is had using 24 especímenes of Oryctolagus cuniculus, to determine the effect of the raw extract of American Persea Mill var. strong in the induced hyperlipidemia. A design experimental growing stimulus has been used, being used biochemical methods and farmacodinámicos to determine the effect hypolipidemy. It was distributed to the specimens in 4 experimentation groups, with 6 animal c/u: Group I (he was not administered treatment), Group II (he was administered lovastatina to the dose of 0.33 mg/Kg p.c.), group III and group IV (they were administered the raw extract of the mesocarpio of american Persea Mill var. strong to the doses of 2.5 g/Kg p.c and 5 g/Kg p.c respectively), for 15 days. Concluding that: The raw extract of the mesocarpio of American Persea Mill var. strong to dose of 2,5 g /Kg p.c. /15 days it possesses effect hypolipidemy.En el presente estudio se han utilizando 24 especímenes de Oryctolagus cuniculus, para determinar el efecto del extracto crudo de Persea americana Mill var. fuerte en la hiperlipidemia inducida. Se ha empleado un diseño experimental estímulo creciente, utilizándose métodos bioquímicos y farmacodinámicos para determinar el efecto hipolipidémico.Se distribuyó a los especimenes en 4 grupos de experimentación, con 6 animales c/u: Grupo I (no se le administró tratamiento), Grupo II (se le administró lovastatina a la dosis de 0.33 mg/Kg p.c.), grupo III y grupo IV (se les administró el extracto crudo del mesocarpio de Persea americana Mill var. fuerte a las dosis de 2.5 g/Kg p.c y 5 g/Kg p.c respectivamente), por 15 días. Concluyendo que: El extracto crudo del mesocarpio de Persea americana Mill var. fuerte a dosis de 2,5 g /Kg p.c./15 días posee efecto hipolipidémico

Diversity and Antimicrobial Potential of Predatory Bacteria from the Peruvian Coastline

The microbiome of three different sites at the Peruvian Pacific coast was analyzed, revealing a lower bacterial biodiversity at Isla Foca than at Paracas and Manglares, with 89 bacterial genera identified, as compared to 195 and 173 genera, respectively. Only 47 of the bacterial genera identified were common to all three sites. In order to obtain promising strains for the putative production of novel antimicrobials, predatory bacteria were isolated from these sampling sites, using two different bait organisms. Even though the proportion of predatory bacteria was only around 0.5% in the here investigated environmental microbiomes, by this approach in total 138 bacterial strains were isolated as axenic culture. 25% of strains showed antibacterial activity, thereby nine revealed activity against clinically relevant methicillin resistant Staphylococcus aureus (MRSA) and three against enterohemorrhagic Escherichia coli (EHEC) strains. Phylogeny and physiological characteristics of the active strains were investigated. First insights into the chemical basis of the antibacterial activity indicated the biosynthetic production of the known compounds ariakemicin, kocurin, naphthyridinomycin, pumilacidins, resistomycin, and surfactin. However, most compounds remained elusive until now. Hence, the obtained results implicate that the microbiome present at the various habitats at the Peruvian coastline is a promising source for heterotrophic bacterial strains showing high potential for the biotechnological production of antibiotics

Identification and heterologous expression of the kocurin biosynthetic gene cluster

The antibiotically bioactive thiopeptide compound kocurin was identified in extracts from a newly isolated Kocuria rosea strain. The axenic strain was retrieved from a soil sample of the intertidal area at the Paracas National Park, Peru. The genetic basis of this promising natural product with activity against methicillin-resistant Staphylococcus aureus (MRSA) strains was revealed by comparative genome analysis of this new isolate and other reported thiopeptide producer strains. The functionality of the predicted gene locus was experimentally proven by heterologous expression in Streptomyces coelicolor M1146. Expression of the gene cluster under the control of a constitutive promoter enabled the transgenic strain to produce kocurin in selected media. The kocurin biosynthetic gene cluster comprises nine open reading frames and spans around 12 kbp of the genome

Chemical Composition and Anthelmintic Activity of the Peruvian Endemic Species Chuquiraga weberbaueri “Amaro” on Sheep Fasciolosis

Chuquiraga weberbaueri “amaro” (Asteraceae) is a plant species popularly used for the treatment of animal fasciolosis in Peru. However, biological and chemical studies of this species remain scarce. Hence, the objective of the present study was to evaluate the anthelmintic effect of the hydroethanolic and aqueous extracts of C. weberbaueri leaves on Fasciola hepatica, through a controlled and a therapeutic efficacy test in artificially infected sheep. In addition, the chemical composition of the extracts was evaluated using phytochemical screening and analysis by UHPLC-MS/MS. Among the evaluated extracts, the aqueous extract proved to be the most efficient and of low toxicity against F. hepatica, inhibiting about 70% of its oviposition, using a 100 mg/kg dose. The chemical study showed that the major constituents were phenolic derivatives, such as chlorogenic acid and ethyl caffeate, with the notable presence of flavonoids, saponins, and lactones. Therefore, it was possible to observe the antihelmintic effect of the C. weberbaueri on the F. hepatica, which suggests the use of this plant extract as a potential alternative in the sheep fasciolosis treatment.Trabajo financiado por fondos concursables de Canon Minero de la Universidad Nacional de Cajamarca, aprobado con Resolución de Consejo Universitario N° 2871, [Número de contrato 2871-2018-UNC