Summary of nucleic acids extraction from eukaryotic microalgae using EMNE method.

<p>Summary of nucleic acids extraction from eukaryotic microalgae using EMNE method.</p

Gel electrophoresis of total RNA extracted from <i>Botryococcus</i> sp by EMNE (A), F&T (B), and BPC method (C).

<p>RNA samples were electrophoresed on a 1.6% agarose gel in 0.5× TAE buffer. Lane M, 1 Kb ladder; lane 1, <i>Botryococcus braunii</i> CCALA 776; lane 2, <i>B. braunii</i> CCALA 777; lane 3, <i>B. braunii</i> CCALA 778; lane 4, <i>B. braunii</i> CCALA 779; lane 5, <i>B. braunii</i> UTEX 572.</p

Cost Effectiveness Analysis of different methods used for DNA extraction.

<p>Baseline scores and relative scores for each parameter for different methods have been depicted.</p

qPCR of <i>B. braunii</i> NIES 836 18 S rRNA gene of 213 bp (A), <i>rBcL</i> gene of 343 bp (B), 18 S rRNA gene of 431 bp (C), and 18 S rRNA gene of 527 bp (D) from DNA of <i>B. braunii</i> sp.

<p>Closed square; P kit, open square; Q kit, closed diamond; F&T method, open diamond; BPC method, closed triangle; EMNE method.</p

RT-qPCR of <i>B. braunii</i> NIES 836 18 S rRNA gene of 213 bp (A), <i>rBcL</i> gene of 343 bp (B), 18 S rRNA gene of 431 bp (C), and 18 S rRNA gene of 527 bp (D).

<p>Closed square; P kit, open square; Q kit, closed diamond; F&T method, open diamond; BPC method, closed triangle; EMNE method.</p

Comparison of quality and quantity of DNA samples extracted from B. <i>braunii</i> using different methods.

a<p>The DNA purity was estimated by calculating the ratio between the absorbance at 260 nm and 280 nm.</p>b<p>The DNA concentration was quantified using a spectrophotometer at 260 nm.</p>c<p>The cultured cells were harvested from culture of 50 ml (commercial kit), 3 ml (BPC & F&T methods), and 1 ml (EMNE method for <i>B. braunii</i>), respectively.</p>d<p>The DNA yield was estimated by calculating the DNA concentration between the dry cell weight and total DNA.</p><p>CCALA, Culture Collection of Autotrophic Organisms; NIES, National Institute for Environmental Studies; UTEX, the Culture Collection of Algae at the University of Texas.</p

Boletín de Segovia: Número 78 - 1923 junio 29

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200

Comparison of yield and purity of RNA samples prepared by EMNE, F&T, and BPC method.

a<p>The RNA purity was estimated by calculating the ratio between the absorbance at 260 nm and 280 nm.</p>b<p>The RNA concentration was quantified using a spectrophotometer at 260 nm.</p>c<p>The cultured cells were harvested from culture of 3 ml (BPC & F&T methods) and 1 ml (EMNE method for <i>B. braunii</i>), respectively.</p><p>CCALA, Culture Collection of Autotrophic Organisms; UTEX, the Culture Collection of Algae at the University of Texas at Austin.</p

Gel electrophoresis of total DNA extracted from <i>B. braunii</i> and other eukaryotic microalgae.

<p>DNA samples were electrophoresed on a 1.6% agarose gel in 0.5× TAE buffer. (a) commercial method 1 (P Kit); (b) commercials method 2 (Q Kit), (c) F&T method; (d) BPC method; (e) EMNE method. Lane M, 1 Kb ladder; lane 1, <i>B. braunii</i> CCALA 776; lane 2, <i>B. braunii</i> CCALA 777; lane 3, <i>B. braunii</i> CCALA 778; lane 4, <i>B. braunii</i> CCALA 779; lane 5, <i>B. braunii</i> UTEX 572; lane 6, <i>B. braunii</i> NIES 836.</p