Additional file 1: of Attenuation of TGFBR2 expression and tumour progression in prostate cancer involve diverse hypoxia-regulated pathways

Figure S1. TGFBR2 expression was significantly reduced in prostate cancer tissues from data of TCGA. Figure S2. Kaplan-Meier overall survival and disease-free survival analysis for EZH2 using PRAD TCGA dataset. Figure S3. EZH2 and TGFBR2 expression were inversely correlated in PCa patients using PRAD TCGA dataset. Figure S4. Bar diagrams which represented the relative protein expression levels of TGFBR2 (A), HIF1A (B), and HIF2A (C) in normoxic or hypoxic condition. Figure S5. Bar diagrams which represented the relative protein expression levels of EZH2 (A), H3k27me3 (B), and TGFBR2 (C) after treating with EZH2 siRNAs or DNZep. Figure S6. Bar diagrams which represented the relative protein expression levels of EZH2 in normoxia or hypoxia. Figure S7. Western blot showed that siRNA decreased the protein expression level of HIF-1a, especially in DU145 and PC3 cells. Figure S8. Bar diagrams which represented the relative protein expression levels of TGFBR2 after miR-93 overexpression or downregulation. Figure S9. Bar diagrams which represented the colony numbers in different groups after miR-93 overexpression or downregulation. Figure S10. Bar diagrams which represented the relative mRNA expression levels of E-cadherin (A), N-cadherin (B), Vimentin (C), Zeb1 (D), and Zeb2 (E) after treating with miR-93 mimics or inhibitor. Table S1. Clinicopathological characters in our cohort of 56 PCa patients. Table S2. Primer list used in this study. (DOC 1410 kb

Distributions of the lncRNAs expression levels.

<p>That most significantly different expressed were validated by qPCR in 63 pairs of RCCC and NT samples.</p

Comparison between microarray data and qPCR result.

<p>ENST00000456816, X91348, BC029135 and NR_024418 determined to be differentially expressed in RCCC samples compared with NT samples in six patients by microarray were validated by qPCR. The heights of the columns in the chart represent the log-transformed median fold changes (T/N) in expression across the six patients for each of the four lncRNAs validated; the bars represent standard errors. The validation results of the four lncRNAs indicated that the microarray data correlated well with the qPCR results.</p

Summary of data from microarray for six pairs of renal cancer and adjacent normal tissues.

<p>Summary of data from microarray for six pairs of renal cancer and adjacent normal tissues.</p

A collection of deregulated lncRNAs detected using microarray in six RCCC patients.

<p>RCCC samples versus NT samples, False discovery rate (FDR)<0.1%, p<0.01.</p