Toxoplasma gondii antibodies in Mexican blood donors [2]

[No abstract available

Toxoplasma gondii antibodies in Mexican blood donors [2]

[No abstract available

Effect of nitaxozanide and pyrimethamine on astrocytes infected by toxoplasma gondii in vitro

Background and Aims: T. gondii is a causal agent of encephalitis in immunocompromised patients. Pyrimethamine (PYR) has been the treatment of choice for toxoplasmosis. The aim of this study was to analyze the effect of nitazoxanide and pyrimethamine on astrocytes infected with T. gondii invitro. Methods: Rat astrocytes were cultured and infected with T. gondii. The effect of nitazoxanide (10, 20 and 30 μg/mL) and pyrimethamine (7, 10 and 13 μg/mL) on astrocytes infected was evaluated at 24 and 48 h post-infection. Tachyzoites and astrocytes were detected by the immunocytochemical method. T. gondii viability in astrocytes infected and treated with NTZ and PYR as well as NTZ and PYR citotoxicity on astrocytes in vitro were evaluated by the MTT assay. Results: The number of parasites in astrocytes treated with the drugs was significantly reduced when compared to control (p <0.001) at 24 and 48 h. Nitazoxanide produced 97% T. gondii death in a concentration of 10 μg/mL in 48 h infected astrocytes. At 48 h, the death rate of T. gondii was higher when treated with nitazoxanide than with pyrimethamine. A higher toxicity rate in astrocyte was observed when using pyrimethamine at 40 μg/mL. Conclusions: Nitazoxanide reduced T. gondii infection more efficiently than pyrimethamine and is not cytotoxic to astrocytes at the administered dose. © 2013 IMSS

Effect of nonionic surfactants on Rhizopus homothallicus lipase activity: A comparative kinetic study

Background and Aims: T. gondii is a causal agent of encephalitis in immunocompromised patients. Pyrimethamine (PYR) has been the treatment of choice for toxoplasmosis. The aim of this study was to analyze the effect of nitazoxanide and pyrimethamine on astrocytes infected with T. gondii invitro. Methods: Rat astrocytes were cultured and infected with T. gondii. The effect of nitazoxanide (10, 20 and 30 ?g/mL) and pyrimethamine (7, 10 and 13 ?g/mL) on astrocytes infected was evaluated at 24 and 48 h post-infection. Tachyzoites and astrocytes were detected by the immunocytochemical method. T. gondii viability in astrocytes infected and treated with NTZ and PYR as well as NTZ and PYR citotoxicity on astrocytes in vitro were evaluated by the MTT assay. Results: The number of parasites in astrocytes treated with the drugs was significantly reduced when compared to control (p <0.001) at 24 and 48 h. Nitazoxanide produced 97% T. gondii death in a concentration of 10 ?g/mL in 48 h infected astrocytes. At 48 h, the death rate of T. gondii was higher when treated with nitazoxanide than with pyrimethamine. A higher toxicity rate in astrocyte was observed when using pyrimethamine at 40 ?g/mL. Conclusions: Nitazoxanide reduced T. gondii infection more efficiently than pyrimethamine and is not cytotoxic to astrocytes at the administered dose. " 2013 IMSS.",,,,,,"10.1016/j.arcmed.2013.07.002",,,"http://hdl.handle.net/20.500.12104/40969","http://www.scopus.com/inward/record.url?eid=2-s2.0-84885154526&partnerID=40&md5=9c480cda80220d78facc189ae6e22aa0",,,,,,"6",,"Archives of Medical Research",,"41

Neural inverse optimal control applied to type 1 diabetes mellitus patients

Anti-Toxoplasma gondii antibodies of all IgG subclasses were studied in mother/newborn pairs. IgG1 in the mothers and IgG3 in the newborns were related to offspring clinical problems; IgG2 and IgG3 in the babies were markers of vertical transmission, and IgG4 in mothers or children were associated to clinical problems. IgG subclasses may be markers of congenital infection or clinical outcome. " 2008 Lippincott Williams & Wilkins.",,,,,,"10.1097/INF.0b013e31816591df",,,"http://hdl.handle.net/20.500.12104/40301","http://www.scopus.com/inward/record.url?eid=2-s2.0-45849091924&partnerID=40&md5=02baced3cfd51a860f9e4144f1a87113",,,,,,"5",,"Pediatric Infectious Disease Journal",,"46

Congenital toxoplasmosis: Specific IgG subclasses in mother/newborn pairs

Anti-Toxoplasma gondii antibodies of all IgG subclasses were studied in mother/newborn pairs. IgG1 in the mothers and IgG3 in the newborns were related to offspring clinical problems; IgG2 and IgG3 in the babies were markers of vertical transmission, and IgG4 in mothers or children were associated to clinical problems. IgG subclasses may be markers of congenital infection or clinical outcome. © 2008 Lippincott Williams & Wilkins

Similar but not equivalent: Ecological niche comparison across closely-related Mexican white pines

Congenital toxoplasmosis is an obstetric problem in Mexico, but its actual frequency is unknown. Using a network for screening of non-infectious disorders, we performed a pilot study to determine the frequency of IgM antibodies to Toxoplasma gondii in 1,003 infants (53.1% male, mean SD age = 18.3 13.0 days, birth weight = 3.116 0.453 kg) in Mexico City from March to April 2003. Blood samples embedded in filter paper were assayed for IgM antibodies using a capture enzyme-linked immunosorbent assay and results were confirmed by Western blot. Two asymptomatic newborns, one of them premature, had IgM and IgG antibodies in a serum sample taken from both the infant and the mother and were clinically followed. Our data suggest a frequency of approximately two cases of congenital T. gondii infection per 1,000 newborns in Mexico City. Copyright " 2005 by The American Society of Tropical Medicine and Hygiene.",,,,,,,,,"http://hdl.handle.net/20.500.12104/44528","http://www.scopus.com/inward/record.url?eid=2-s2.0-15844385662&partnerID=40&md5=e7819698cbdc45b508568945637e491e",,,,,,"2",,"American Journal of Tropical Medicine and Hygiene",,"14

Short report: Neonatal screening pilot study of Toxoplasma gondii congenital infection in Mexico

Congenital toxoplasmosis is an obstetric problem in Mexico, but its actual frequency is unknown. Using a network for screening of non-infectious disorders, we performed a pilot study to determine the frequency of IgM antibodies to Toxoplasma gondii in 1,003 infants (53.1% male, mean ± SD age = 18.3 ± 13.0 days, birth weight = 3.116 ± 0.453 kg) in Mexico City from March to April 2003. Blood samples embedded in filter paper were assayed for IgM antibodies using a capture enzyme-linked immunosorbent assay and results were confirmed by Western blot. Two asymptomatic newborns, one of them premature, had IgM and IgG antibodies in a serum sample taken from both the infant and the mother and were clinically followed. Our data suggest a frequency of approximately two cases of congenital T. gondii infection per 1,000 newborns in Mexico City. Copyright © 2005 by The American Society of Tropical Medicine and Hygiene