A STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DAUNORUBICIN AND CYTARABINE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Objective: A simple, accurate, and precise method was developed for the simultaneous estimation of daunorubicin and cytarabine dosage form using reverse-phase HPLC and validated with different parameters such as accuracy, precision, repeatability, linearity, limit of detection (LOD), and limit of quantitation (LOQ) as per ICH Q2R1 guidelines. Methods: The chromatogram was run through Agilent C18 Column of dimensions 150×4.6 mm, 5 m. Mobile phase containing 0.01N KH2PO4: Methanol taken in the ratio 50:50 was pumped through the column at a flow rate of 1.0 ml/min. The temperature was maintained at 30°C. The optimized wavelength selected was 240 nm. Results: Retention times for daunorubicin and cytarabine were found to be 2.433 min and 3.045 min. The %RSD of the daunorubicin and cytarabine was found to be 0.7 and 0.4, respectively. The %Recovery was obtained as 99.96% and 100.40% for daunorubicin and cytarabine, respectively. LOD and LOQ values obtained from regression equations of daunorubicin and cytarabine were 0.08 μg/ml, 0.24 μg/ml and 0.94 μg/ml, 2.86 μg/ml, respectively. The regression equation for daunorubicin is y=28587x+3141 and y=35995x+37534 for cytarabine. Conclusion: The method developed was simple and economical that can be adopted in regular quality control tests in industries

METHOD DEVELOPMENT, VALIDATION AND STABILITY STUDIES FOR THE DETERMINATION OF LURASIDONE HYDROCHLORIDE IN BULK AND TABLET DOSAGE FORM BY RP-HPLC

Objective: To develop a simple, rapid, sensitive, precise, accurate, economical and validated reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of lurasidone hydrochloride in tablet dosage form. Methods: The chromatographic separation was carried out on a prontosil C18, AQ (100 mm×4.6 mm, 3 µm) column. A mixture of phosphate buffer (pH 3.0): acetonitrile (ACN) (55:45v/v) was used as a mobile phase. Flow rate of 1.0 ml/min and 10 μl injection volume was used for the assay. PDA detector was used, and the detection wavelength was 230 nm. The retention time (RT) of lurasidone hydrochloride was found to be 4.505±0.01 min. The method was validated according to the ICH guidelines. Results: The calibration curve for lurasidone hydrochloride was linear with a correlation coefficient value 0.999 in the concentration range of 25-125%. Specificity, accuracy (% mean recovery, 99.08%), precision, detection limits, robustness (% RSD˂2) and system suitability were found to be within limits. Degradation studies were performed under different stressed conditions, and the results of degradation studies reveal that the developed method was stable. Conclusion: The developed method was simple, reliable, economical and stable and it can be applied for the routine quality control analysis of lurasidone hydrochloride in tablet dosage forms

Antiviral activity of leaf-bud gum-resin of Tarenna asiatica

The leaf-bud exudate of Tarenna asiatica (Rubiaceae: Ixoroideae, Pavetteae) is investigated for its biological activity. The crude benzene extract and corymbosin (pure compound isolated) were screened for antiviral activity by using ELISA and PCR methods against animal (blue tongue and chikungunya) and plant (papaya ring spot, sesbania mosaic and common bean mosaic) viruses. Both corymbosin and benzene extract showed significant antiviral activity though corymbosin was found relatively more potent against the animal and plant viruses tested. This is the first report of antiviral activity for the gum-resin of T. asiatica, so also for the compound corymbosin, against the plant viruses

Phytochemical investigation and evaluation of antibacterial and antioxidant activities of leaf-bud exudate of <i>Tarenna asiatica </i><span style="color:black;mso-ansi-language:IT; mso-bidi-font-style:italic" lang="IT">(L.) Kuntze ex K. Schum.<span style="mso-ansi-language:IT" lang="IT"> </span></span>

48-51Tarenna asiatica (L.) Kuntze ex K. Schum. (Magnoliophyta: Rubiaceae) is traditionally used as anthelmintic, antiseptic, antiulcer and to promote suppuration. The leaf-bud exudates collected from the local forests was extracted with benzene by maceration. Preliminary chemical tests were conducted for select secondary metabolites aside isolating the known flavone, corymbosin. Employing the cup-plate method, different concentrations of benzene extract and corymbosin were screened against Bacillus sphaericus<span style="mso-bidi-font-style: italic">, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus, using streptomycin as the standard drug. The extract evinced a weak to moderate activity against all the strains tested while corymbosin was inactive. The antioxidant activity of the benzene extract was studied by nitric oxide scavenging activity, reduction of DPPH free radical, iron-induced lipid peroxidation and superoxide scavenging activity, with ascorbic acid as the standard drug. The extract was found to be IC50 in the range of 20-60 g/mL in the assays performed. </span

Phytochemical investigation and evaluation of antibacterial and antioxidant activities of leaf-bud exudate of Tarenna asiatica (L.) Kuntze ex K. Schum.

Tarenna asiatica (L.) Kuntze ex K. Schum. (Magnoliophyta: Rubiaceae) is traditionally used as anthelmintic, antiseptic, antiulcer and to promote suppuration. The leaf-bud exudates collected from the local forests was extracted with benzene by maceration. Preliminary chemical tests were conducted for select secondary metabolites aside isolating the known flavone, corymbosin. Employing the cup-plate method, different concentrations of benzene extract and corymbosin were screened against Bacillus sphaericus, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus, using streptomycin as the standard drug. The extract evinced a weak to moderate activity against all the strains tested while corymbosin was inactive. The antioxidant activity of the benzene extract was studied by nitric oxide scavenging activity, reduction of DPPH free radical, iron-induced lipid peroxidation and superoxide scavenging activity, with ascorbic acid as the standard drug. The extract was found to be IC50 in the range of 20-60 g/mL in the assays performed