Amérique : Antilles : Saint-Christophe-et-Niévès : Saint-Christophe : Saint-George Basseterre : Rue de Basseterre

Légende manuscrite sur le document original : "Saint-Christophe (Saint Kitts). Basseterre. " Géolocalisation : approximative centrée sur Basseterre

Overproduced Sch9-YFP forms distinct fluorescent foci.

<p>Scale bar indicates 5 μm. BF, bright-field microscopy. Dashed lines separated different fields of view.<i>WT</i> and <i>Δ</i> designate BY4742 and <i>sch9Δ</i>-BY4741 strains, respectively. Arrowheads mark elongated cells.</p

Nothern blotting was used to assess the effect of deletion on the accumulation of mRNA

<p><b>Copyright information:</b></p><p>Taken from "Inactivation of NMD increases viability of nonsense mutants in "</p><p>http://www.biomedcentral.com/1471-2199/8/71</p><p>BMC Molecular Biology 2007;8():71-71.</p><p>Published online 16 Aug 2007</p><p>PMCID:PMC2039749.</p><p></p> Total RNA was isolated from strain 5B-D1645 () transformed with plasmids pRS316 and pRS316/, designated as () and (), respectively. Northern blots were hybridized with radiolabeled and probes. . Representative hybridization signals specific to mRNA (upper panel), mRNA (middle panel) and actin mRNA () used as an internal control (lower panel) are shown. Numbers indicated under upper and middle panels represent the relative abundance of and mRNA's, respectively, in and strains. (s.d.) – standard deviation. . Accumulation of precursor mRNA was used to control that NMD is altered in the strain. The probe detects both precursor and mature mRNA. The fold increase in precursor/mature mRNA accumulation in strain relative to strain is indicated with the standard deviation (s.d.)