Separation And Characterization Of The Hemoglobin Components Of Pterygoplichthys Pardalis, The Acaribodo

1. 1. The four main hemoglobin components of the hemolysate of Pterygoplichthys pardalis have been isolated and characterized. 2. 2. The functional properties investigated for the isolated components comprise the effect of pH and ATP on (i) the O2 equilibrium, (ii) the O2 dissociation kinetics, (iii) the CO combination kinetics. 3. 3. Component I, corresponding to approx 50% of the total hemoglobin, is characterized by functional properties which are distinctly different from those of Components II, III and IV, which are alike 4. 4. Thus it is shown, once more, that multiple components in an hemolysate fall into categories of hemoglobins characterized by distinct and complementary functional properties. © 1979.621173177IEEE Communications Society,IEEE Computer SocietyAntonini, Brunori, (1971) Hemoglobin and Myoglobin in their Reactions with Ligands, , North-Holland, AmsterdamBrunori, Molecular adaptation to physiological requirements (1975) Curr. Top. Cell. Regulation, 9, pp. 1-39Brunori, Giardina, Antonini, Benedetti, Bianchini, (1974) J. molec. Biol., 86, pp. 165-169Bunn, Riggs, The measurement of the Bohr effect of fish hemoglobins by gel electrofocusing (1979) Comp. Biochem. Physiol., 62 A, pp. 95-100Fink, Fink, Central Amazonia and its fishes (1979) Comp. Biochem. Physiol., 62 A, pp. 13-30Fyhn, Fyhn, Davis, Powers, Fink, Garlick, Hemoglobin heterogeneity in Amazonian fishes (1979) Comp. Biochem. Physiol., 62 A, pp. 39-66Greenwood, Rosen, Weitzman, Myers, Phyletic studies of Teleostean fishes with a provisional classification of living forms (1966) Bull. Am. Mus. Nat. Hist., 131, pp. 339-455Martin, Bonaventura, Brunori, Fyhn, Fyhn, Garkick, Powers, Wilson, The isolation and characterization of the hemoglobin components ofMylossoma sp., an Amazonian teleost (1979) Comp. Biochem. Physiol., 62 A, pp. 155-162Riggs, Wolbach, Sulfhydryl groups and the structure of hemoglobin (1956) The Journal of General Physiology, 39, pp. 585-605Washburn, (1926) International Critical Tables of Numerical Data, Physics, Chemistry and TechnologyWeber, Wood, Effects of erythrocyte nucleoside triphosphates on oxygen equilibria of composite and fractionated hemoglobins from the facultative air-breathing Amazonian Catfish,Hypostomus andPterygoplichthys (1978) Comp. Biochem. Physiol., 62 A, pp. 179-18