Use of ultraviolet laser ablation electrospray ionization mass spectrometry for plant metabolite imaging

This dissertation describes a variety of plant studies by laser ablation electrospray ionization mass spectrometry imaging. (LAESI MSI). During the LAESI process, a plant sample is ablated when a laser is fired at it. The resultant neutral plume is ionized and enters the mass spectrometer by an electrospray stream. In all experiments an ultraviolet (UV; 355 Nd:YAG) laser was used. Once ions enter the mass spectrometer and are detected, a spectrum is produced for a particular area on the plant. The rest of the plant is ablated and spectra are combined for image creation. Unlike other forms of mass spectrometry, MSI allows for chemical and spatial information which can help understand the diversity of plants, where one cell may contain different chemicals compared to its neighboring cell. Plants studied in this dissertation included peonies, coleus, and Arabidopsis thaliana. A variety of metabolites were imaged, including flavonoids, anthocyanins and lipids. Analyte images allowed further insight into biological processes, such as the malonylshisonin pathway in coleus leaves, and effects of lipids during autophagy in Arabidopsis thaliana. LAESI MSI was also shown in this dissertation to be a potentially faster more sensitive technique than other forms of mass spectrometry

GPS and Total Station Plane Survey of the Unexcavated 24BE2206 Site in the Big Hole Valley of Montana

Archaeological excavations reveal size and social structure, subsistence strategies, time of occupation & site usage for past human groups. When materials are removed from the excavation site, their provenience can be lost if not carefully recorded.https://digitalcommons.mtech.edu/urp_aug_2017/1004/thumbnail.jp

Experience with a high-brightness storage ring: the NSLS 750 MeV vuv ring

The NSLS vuv ring is the first implementation of the proposals of R. Chasman and G.K. Green for a synchrotron radiation source with enhanced brightness: its lattice is a series of achromatic bends with two zero-gradient dipoles each, giving small damped emittance; and these bends are connected by straight sections with zero dispersion to accommodate wigglers and undulators without degrading the radiation damping properties of the ring. The virtues of the Chasman-Green lattice, its small betatron and synchrotron emittances, may be understood with some generality; e.g. the electron ..gamma..m/sub 0/c/sup 2/ energy and the number of achromatic bends M sets a lower limit on the betatron emittance of e/sub x/ > 7.7 x 10/sup -13/ ..gamma../sup 2//M meter-radians. There is strong interest in extrapolation of this type of lattice to 6 GeV and to 32 achromatic bends. The subject of this report is the progress toward achieving performance in the vuv ring limited by the radiation damping parameters optimized in its design. 14 refs., 4 figs., 1 tab

Linac coherent light source: status and prospects

The Linac Coherent Light Source (LCLS) Project will be an x-ray free-electron laser. It is intended to produce pulses of 800-8,000 eV photons. Each pulse, produced with a repetition frequency of up to 120 Hz, will provide >10{sup 12} photons within a duration of less than 200 femtoseconds. The project employs the last kilometer of the SLAC linac to provide a low-emittance electron beam in the energy range 4-14 GeV to a single undulator. Two experiment halls, located 100m and 350m from the undulator exit, will house six experiment stations for research in atomic/molecular physics, pump-probe dynamics of materials and chemical processes, x-ray imaging of clusters and complex molecules, and plasma physics. Engineering design activities began in 2003, and the project is to be completed in March 2009. The project design permits straightforward expansion of the LCLS to multiple undulators

Status of the advanced photon source

This report presents general information on the Advanced Photon Source (APS) and then breaks down the APS project into three categories: accelerator systems, experimental facilities, and conventional facilities. The accelerator systems consist of the 7 GeV APS positron storage ring and a 7 GeV positron injector. The experimental facilities include 20 undulator radiation sources and the x-ray beamline components necessary to transport their extraordinarily intense x-ray beams outside the accelerator enclosure. Also included are x-ray beamline components for 20 bending magnet radiation sources. The conventional facilities consist of the accelerator enclosures, a 35,300 m{sup 2} experimental hall to house the x-ray beamlines, an office building for the APS staff and lab/office facilities for the research groups which will construct and operate the first 40 beamlines. APS users are described, and the properties of synchrotron radiation are discussed

The problem of the deficit of motive activity of students of the law College

The article presents the results of the analysis and evaluation of physical fitness level of first-year students law College, depending on the amount of physical activity in schoolВ статье представлены результаты проведенного анализа и оценки уровня физической подготовленности студентов-первокурсников юридического колледжа в зависимости от объема двигательной активности в школ

An X-ray Absorption Edge Detector for High-Resolution Measurement of Undulator Effective K-Parameter

The spectrum of angle-integrated undulator radiation displays a sharp edge at every harmonic photon energy. A technique utilizing this feature to measure minute changes in K-parameters of an undulator in a free-electron laser has been proposed. To date, this technique requires the use of crystal monochromators as bandpass filters whose energy centroid depends on the incident angle of the x-ray beam. In this work we propose to use the absorption edge of an appropriate element as an energy-selective detector whose response is truly independent of the angle of the x-ray beam, and hence independent of electron beam direction and emittance. We will discuss the basic design concept of the detection system and illustrate its projected performance with computer simulations

Method specific fitness training in medical group

Fitness classes are widespread among the students, but for people with chronic diseases, there are many restrictions to physical exercise. Using a large arsenal of methods of training, exercise selection, assumptions, simulators can be adapted according to the specific classes of disease, the level of physical and functional condition of the studentЗанятия фитнесом широко распространены в студенческой среде, но для людей с хроническими заболеваниями существует много запретов к занятиям физическими упражнениями. Используя большой арсенал методик тренировок, выбора упражнений, исходных положений, тренажеров, можно адаптировать занятия исходя из специфики заболевания, уровня физического и функционального состояния студент

The advanced photon source low-energy undulator test line

There are a number of fully commissioned 3rd-generation synchrotron light sources in the world today. So far they have met the demanding requirements of the user community; however, there is always a desire to go beyond what is presently available or even desirable. The Advanced Photon Source (APS) Low-Energy Undulator Test Line (LEUTL) was conceived to address the advancement of synchrotron light sources. The LEUTL uses the existing APS linac and a low-emittance electron gun, and by means of measurements of the beam and generated light, will test new and innovative undulators and push the technology and physics of single-pass, coherent light sources. The design and status of the LEUTL will be described along with its immediate capabilities and those planned for the future