Obesity and brain cancer: proteomic analyzes of the influence of the adipocyte secretome on glioma Gl261 cells

Glioma is the most frequent form of malignant brain tumor in the adults and childhood. There is a global tendency toward a higher incidence of gliomas in highly developed and industrialized countries. Simultaneously obesity is reaching epidemic proportions in such developed countries. It has been highly accepted that obesity may play an important role in the biology of several types of cancer. We have developed an in vitro method for the understanding of the influence of obesity on glioma mouse cells (Gl261).info:eu-repo/semantics/publishedVersio

Sea warming affects bream (Sparus aurata) tissues and stress proteins (HSP70)

The aim of the present study is to assess the stress response of a marine fish, sea bream (Sparus aurata) exposed to increasing water temperature and different water acidity (alone or in combination). Here we present preliminary data on temperature effects on S. aurata at a cellular and molecular level. In addition, tissue samples from muscle, livers, gills and intestine are examined to evaluate any alterations caused by altering this physical parameter

Testing the variability of PSA expression by different human prostate cancer cell lines by means of a new potentiometric device employing molecularly antibody assembled on graphene surface

Prostate Specific Antigen (PSA) is widely used as a biomarker for prostate cancer. Recently, an electrochemical biosensor for PSA detection by means ofmolecularly imprinted polymers (MIPs)was developed. Thiswork evaluated the performance and the effectiveness of that PSA biosensor in screening the biomarker PSA in biological mediawith complex composition, collected fromdifferent human prostate cell line cultures. For that, the prostate cancer LNCaP and PC3 cells, and the non-cancerous prostate cell line PNT2 were cultured for 2, 7 and 14 days in eitherα-MEMor RPMI in the presence of 10% or 30% fetal bovine serum. Human gingival fibroblastswere used as a non-cancerous non-prostatic control. The different culture conditions modulated cellular proliferation and the expression of several prostate markers, including PSA. The electrochemical biosensor was able to specifically detect PSA in the culture media and values obtained were similar to those achieved by a commercial Enzyme- Linked Immunosorbent Assay (ELISA) kit, the most commonly used method for PSA quantification in prostate cancer diagnosis. Thus, the tested biosensor may represent a useful alternative as a diagnostic tool for PSA determination in biological samples.info:eu-repo/semantics/publishedVersio