Genetic diversity assessment in pea cultivars and lines using the SSR analysis

Background. Pea is the main leguminous crop in the Republic of Bashkortostan and widespread all over the world. The key role in the breeding of new pea cultivars is played by source material representing the phenotypic and genotypic diversity of Pisum sativum L., searched for in plant genetic resources collections. SSR markers are successfully used to study the DNA polymorphism of various genetic objects, including pea. However, the distribution of a number of microsatellite alleles in the genotypes of specific lines and cultivars of this valuable pulse crop remains practically unexplored.Materials and methods. Molecular genetic polymorphism was studied in 40 pea cultivar accessions of different ecological and geographical origin from the Vavilov Institute’s genebank of plant genetic resources or developed at regional breeding centers. Microsatellite analysis was performed using 5 SSR markers from the genomic library of microsatellites (Agrogene®, France).Results. All markers delivered good electrophoretic profiles and helped to amplify a number of alleles per locus varying from 2 (AB53) to 9 (AA355). The total number of alleles was 26, while the average number of alleles per locus was 5.2. The polymorphism information content (PIC) varied from 0.39 for locus AB53 to 0.82 for locus AA355, with the mean value of 0.60. The set of SSR markers used in the work made it possible to individualize each of the studied pea genotypes. The measured genetic distances were used to draw a dendrogram showing the distribution of genotypes according to their genetic relationship.Conclusion. Through studying the source material for pea breeding by the SSR analysis the data were obtained that provide additional information about the genetic structure of the collection and the polymorphism of the studied cultivar accessions. The results of genotyping pea cultivars and lines can be used for their genetic identification or to select parental pairs for hybridization

Development of source material for pea breeding through chemical mutagenesis and evaluation of its genetic diversity using SSR markers

Background. Pea (Pisum sativum L.) is a valuable leguminous crop of worldwide importance. The main problem of modern plant breeding is a decrease in the genetic diversity of crops, including pea. One of the ways to increase genetic polymorphism is the use of chemically induced mutagenesis. Sodium azide (NaN3) is a highly effective chemical mutagen successfully used in mutation breeding to increase the productivity of cultivated plants and enrich them with new useful traits. We used it to obtain new pea breeding material.Materials and methods. Experiments were carried out to obtain pea mutants using sodium azide at the concentrations of 1, 5 and 10 mM and the exposure time of 3 and 9 h. Molecular genetic polymorphism of the М2 plants and the original cultivar was assessed using 10 SSR markers from the microsatellite genomic library (Agrogene®, France).Results. Optimal concentrations of sodium azide and the duration of seed treatment with it were identified: 1–5 mM for 3 h. Sixteen mutant populations were obtained; in ten of them a change in the leaf type was found. An analysis of the yield structure components revealed a significant superiority (p < 0.05) over the initial cultivar ‘Pamyati Khangildina’ in the mutant populations No. 1, No. 5, No. 9, No. 10, No. 15 and No. 16 in the number of seeds per pod, No. 9 and No. 16 in the weight of 1000 seeds, and No. 16 in the weight of seeds per plant. A dendrogram constructed on the basis of the SSR analysis data showed the degree of differences between the M2 populations of pea plants and the initial cultivar ‘Pamyati Khangildina’.Conclusion. The obtained mutant populations are planned to be used in pea breeding as sources of high seed numbers in pods, seed yield, seed weight per plant, and large seed size. A microsatellite analysis with 10 SSR markers revealed differences among the M2 mutant populations at the genetic level and made it possible to identify them

Neurological syndromes and "red flags" indicating the cancer process

Neurological symptoms can be the first manifestations of the oncological process and make their debut earlier than the symptoms of a malignant disease. This article shows the" red flags" of neurology, which can become the first symptoms of oncological pathology, i.e., make their clinical debut earlier than the oncological process.Неврологические симптомы могут являться первыми проявлениями онкологического процесса и дебютировать раньше, чем симптомы злокачественного заболевания. В данной статье проведен анализ 54 источников литературы для выявления потенциальных неврологических симптомов - «красных флагов», которые могут являться первыми симптомами онкологической патологии, т.е. дебютировать клинически раньше, чем онкологический процесс

Improvement of a socio-psychological climate in a municipal autonomous educational institution secondary school

The article presents the analysis and assessment of the sociopsychological climate, which is an important aspect that affects the performance of the teaching staff, and gives recommendations for its improvement.В статье представлены анализ и оценка социально-психологического климата, являющегося важным аспектом, влияющим на работоспособность педагогического коллектива, и даны рекомендации по его совершенствованию

Pedagogical managerial mechanisms of the labor market needs in a new generation of specialists

© 2016, Econjournals. All rights reserved.Supply and demand are implemented in the competition between workers for engaging in a particular workplace or work performing, as well as among employers to attract the necessary labor force as a commodity, possessing intelligence, competence, qualification. Under these circumstances, a graduate of an educational institution is not in a favorable position for himself, because he does not have the expected by the customers, practical experience. The paper is aimed at scientific justification of pedagogical mechanisms of management of labor market needs in specialists of a new generation. The leading method in the study of this problem is the comparative method identifying pedagogical mechanisms of supply and demand management in specialists of the new generation in the labor market. The paper presents the results of significant increase fixing in the number of youth unemployment that requires the use of preventive and corrective measures in the management of the needs of the labor market. The authors revealed the structure and content of an innovative model of educational institutes’ specialist graduates, including the structure of the individual; personal qualities; managerial roles; formulation of objectives; analysis, examination; the integration of new management ideas; the use of modern information and others. Presented in the article model of training of a competent professional, expected by labor market, also includes the curriculum (list of subjects, number of hours), requirements for the competence of each academic subject, and indicators and criteria of training quality, the detailed content of the curricula, scientific-methodical maintenance of educational process

Features of a medical interview: the problem

In this article set forth such tasks of educating student - future doctors - as responding to emotions and psycho-social themes of patients within the broader goal of shaping the communicative competence of a doctor.В статье представлены предпосылки проблем обучения студентов – будущих врачей реагированию на эмоции и психо-социальные факторы пациентов в рамках формирования коммуникативной компетентности врача

Prevalence of vitamin D deficiency in patients with spinal cord injury at admission: a single-centred study in the UK

Vitamin D deficiency is prevalent in patients with chronic spinal cord injury (SCI) and has been implicated as an aetiologic factor of osteoporosis and various skeletal and extra-skeletal issues in SCI patients. Few data were available regarding vitamin D status in patients with acute SCI or immediately assessed at hospital admission. This retrospective cross-sectional study evaluated vitamin D status in SCI patients at admission to a UK SCI centre in January–December 2017. A total of 196 eligible patients with serum 25(OH)D concentration records at admission were recruited. The results found that 24 % were vitamin D deficient (serum 25(OH)D < 25 nmol/l), 57 % of the patients had serum 25(OH)D < 50 nmol/l. The male patients, patients admitted in the winter–spring time (December–May), and patients with serum sodium < 135 mmol/l or with non-traumatic causes had a significant higher prevalence of vitamin D deficiency than their counterparts (28 % males v. 11⋅8 % females, P = 0⋅02; 30⋅2 % in winter–spring v. 12⋅9 % in summer–autumn, P = 0⋅007; 32⋅1 % non-traumatic v. 17⋅6 % traumatic SCI, P = 0⋅03; 38⋅9 % low serum sodium v. 18⋅8 % normal serum sodium, P = 0⋅010). There was a significant inverse association of serum 25(OH)D concentration with body mass index (BMI) (r = −0⋅311, P = 0⋅002), serum total cholesterol (r = −0⋅168, P = 0⋅04) and creatinine concentrations (r = −0⋅162, P = 0⋅02) that were also significant predictors of serum 25(OH)D concentration. Strategies for systematic screening and efficacy of vitamin D supplementation in SCI patients need to be implemented and further investigated to prevent the vitamin D deficiency-related chronic complications

Development strategy of service sector in conditions of federal states entities autonomy increasing

© 2016, Econjournals. All rights reserved.The relevance of the study is caused by the necessity to develop the sphere of services as a component of balanced socio-economic development of Federal State’s territories. The purpose of the paper is to define strategies for the development of services in conditions of increasing independence of the Federal State’s entities. A leading approach is the institutional one that considers the strategy of development of service sector in the Federal State as long-term programs of transition to innovative socially oriented type of economic development, reducing interregional differentiation in the level and quality of life of the population, creating equal opportunities for citizens and promoting human development. In the conditions of Federal State entities’ autonomy increasing the strategy of development in the sphere of services consists of citizens’ life conditions and social environment’ quality improving; accelerated development of human potential; competitiveness improving of human and ensuring it economic social sectors’ capital; development of competitive service markets; improving of the efficiency of politico-legal institutions providing social services to the population. Strategies’ defining for the development of services in the conditions of Federal State entities’ autonomy increasing is based on the principles of subsidiarity, sustainability, selectivity, fractals and transvers. Certain strategies of development of services are aimed at reducing territorial socio-economic differentiation to the level, caused by objective differences of the Federation’s entities, as well as ensuring the balance of the revenue base and expenditure commitments

Метод совместной кластеризации в графовом и корреляционном пространствах

Network algorithms are often used to analyze and interpret the biological data. One of the widely used approaches is to solve the problem of identifying an active module, where a connected subnetwork of a biological network is selected which best reflects the difference between the two considered biological conditions. In this work this approach is extended to the case of a larger number of biological conditions and the problem of the joint clustering in network and correlation spaces is formulated.To solve this problem, an iterative method is proposed at takes as the input graph G and matrix X, in which the rows correspond to the vertices of the graph. As the output, the algorithm produces a set of subgraphs of the graph G so that each subgraph is connected and the rows corresponding to its vertices have a high pairwise correlation. The efficiency of the method is confirmed by an experimental study on the simulated data.Алгоритмы на графах часто используются для анализа и интерпретации биологических данных. Одним из широко используемых подходов является решение задачи поиска активного модуля, в которой в графе биологических взаимодействий выделяется связный подграф, лучше всего отражающий разницу между двумя рассматриваемыми биологическими состояниями. В настоящей работе этот подход расширяется на случай большего числа биологических состояний и формулируется задача совместной кластеризации в графовом и корреляционном пространстве.Для решения этой задачи предлагается итеративный метод, принимающий на вход граф G и матрицу X, в которой строки соответствуют вершинам графа. На выходе алгоритм выдает набор подграфов графа G так, что каждый подграф является связным и строки, соответствующие его вершинам, обладают высокой попарной корреляцией.Эффективность метода подтверждается экспериментальным исследованием на смоделированных данных

Commitment of the adult population (parents) and children to vaccine prevention under the conditions of the spread of new coronaviral infection COVID-2019

The work shows the level of awareness and attitude of parents, children and adolescents to vaccination as a method of preventing infectious diseases, an analysis of the vaccination of adults and children against influenza was carried out using the example of the studied sample over the past 5 years, and the readiness of the selected contingent to be vaccinated against coronavirus infection was assessed (COVID-19).В работе показаны уровень осведомленности и отношение родителей, детей и подростков к вакцинации, как к методу профилактики инфекционных заболеваний, проведен анализ привитости взрослого и детского населения от гриппа на примере изучаемой выборки за последние 5 лет, оценена готовность выбранного контингента к прививкам против коронавирусной инфекции (COVID-19