Highlights on the Effect of Somatic Cell Count on Some Milk Constituents

Increasing somatic cell counts (SCC) in milk has a great effect on the chemical characteristics of milk especially fat, protein, lactose, and casein. So, rapid detection of these changes lead to great improvement of milk investments as, it facilitates mastitis control process. For rapid detection of these changes, Bacsomatic™ instrument was used for SCC determination and Milkoscan™ for determination of fat, protein, lactose, and casein percent. There were significance differences between SCC and fat, protein, lactose and casein as p≤0.0001. Also, there were negative correlation (r) between SCC and fat, lactose and casein percent while, there was a positive correlation (r) between SCC and protein. So, control measures for prevention of mastitis, increased SCC (scc/ml) and monitoring were suggested to control changes in chemical constituents of milk

Isolation and Identification of Food Poisoning Bacteria from some Dairy Farms in El-Menoufia Governorate using VITEK 2

Because milk is rich in nutrients, it creates an environment conducive to the growth of bacteria that could be harmful to consumers. Therefore, assessing of its bacteriological quality and the resistance of these pathogens to several antibiotic groups is very important. VITEK 2 compact detected E. coli, Salmonellae spp., S. aureus and B. cereus in the examined samples while it failed to detect Listeria spp. The serological identification of E. coli showed the presence of O44: H18, O127: H6, O159, O15: H2, and O91: H21. Also, Salmonella serotypes as           S. enteritidis (25%), S. infantis (12.5%), S. kentucky (12.5%), S. montevideo (6.25%), S. shangani (12.5%), S. tsevie (12.5%). S. typhimurium (18.75%).  Furthermore, the entero-toxigenic strains of S. aureus were 31.25% of the identified strains; the percentage of entero-toxigenic strains that secrete A, A&C and D enterotoxin were 60%, 20% and (20%); respectively. Ampicillin, amoxicillin / clavulanic acid, cefpodoxime, cefovecin, ceftiofur, and trimethoprim / sulfamethoxazole were all ineffective against the isolated E. coli strains. Intermediate sensitive to cefalothin while they were sensitive to cefalexin, imipenem, amikacin, gentamicin, neomycin, enrofloxacin, marbofloxacin, pradofloxacin, doxycycycline, tetracycline, nitrofurantoin and chloramphenicol. Additionally, the isolated S. aureus exhibited resistance to tetracycline, benzylpenicillin, oxacillin, gentamycin, ciprofloxacin, levofloxacin, and moxifloxacin also; it showed intermediate resistance to rifampicin. The identified S. aureus strains were also susceptible to linezolid, nitrofurantoin, vancomycin, tigecycline, and trimethoprim / sulfamethoxazole