Light scattering from a rough metal surface: theory and experiment

There is still great interest in the determination of microtopographic properties of rough metallic surfaces from light scattering measurements. According to Beckmann–Kirchhoff theory a clear relationship is established between the in-plane angular scattered light intensity and the statistical properties of the surface. We discuss one way to invert this relationship, and we introduce a new iterative procedure to retrieve the height autocorrelation function even for a very rough metallic surface (rms surface roughness of the same order of the optical wavelength). The procedure is eventually applied to the experimental data of a known metallic surface for validation

A bioprinted cardiac patch composed of cardiac-specific extracellular matrix and progenitor cells for heart repair

Congenital heart defects are present in 8 of 1000 newborns and palliative surgical therapy has increased survival. Despite improved outcomes, many children develop reduced cardiac function and heart failure requiring transplantation. Human cardiac progenitor cell (hCPC) therapy has potential to repair the pediatric myocardium through release of reparative factors, but therapy suffers from limited hCPC retention and functionality. Decellularized cardiac extracellular matrix hydrogel (cECM) improves heart function in animals, and human trials are ongoing. In the present study, a 3D-bioprinted patch containing cECM for delivery of pediatric hCPCs is developed. Cardiac patches are printed with bioinks composed of cECM, hCPCs, and gelatin methacrylate (GelMA). GelMA-cECM bioinks print uniformly with a homogeneous distribution of cECM and hCPCs. hCPCs maintain >75% viability and incorporation of cECM within patches results in a 30-fold increase in cardiogenic gene expression of hCPCs compared to hCPCs grown in pure GelMA patches. Conditioned media from GelMA-cECM patches show increased angiogenic potential (>2-fold) over GelMA alone, as seen by improved endothelial cell tube formation. Finally, patches are retained on rat hearts and show vascularization over 14 d in vivo. This work shows the successful bioprinting and implementation of cECM-hCPC patches for potential use in repairing damaged myocardium

New perspectives to repair a broken heart

The aim of cardiac cell therapy is to restore at least in part the functionality of the diseased or injured myocardium by the use of stem/ progenitor cells. Recent clinical trials have shown the safety of cardiac cell therapy and encouraging efficacy results. A surprisingly wide range of non-myogenic cell types improves ventricular function, suggesting that benefits may result in part from mechanisms that are distinct from true myocardial regeneration. While clinical trials explore cells derived from skeletal muscle and bone marrow, basic researchers are investigating sources of new cardiomyogenic cells, such as resident myocardial progenitors and embryonic stem cells. In this commentary we briefly review the evolution of cell-based cardiac repair, some progress that has been made toward this goal, and future perspectives in the regeneration of cardiac tissue. © 2009 Bentham Science Publishers Ltd

Evidence for mechanisms underlying the functional benefits of a myocardial matrix hydrogel for post-MI treatment

Background There is increasing need for better therapies to prevent the development of heart failure after myocardial infarction (MI). An injectable hydrogel derived from decellularized porcine ventricular myocardium has been shown to halt the post-infarction progression of negative left ventricular remodeling and decline in cardiac function in both small and large animal models. Objectives This study sought to elucidate the tissue-level mechanisms underlying the therapeutic benefits of myocardial matrix injection. Methods Myocardial matrix or saline was injected into infarcted myocardium 1 week after ischemia-reperfusion in Sprague-Dawley rats. Cardiac function was evaluated by magnetic resonance imaging and hemodynamic measurements at 5 weeks after injection. Whole transcriptome microarrays were performed on RNA isolated from the infarct at 3 days and 1 week after injection. Quantitative polymerase chain reaction and histologic quantification confirmed expression of key genes and their activation in altered pathways. Results Principal component analysis of the transcriptomes showed that samples collected from myocardial matrix-injected infarcts are distinct and cluster separately from saline-injected control subjects. Pathway analysis indicated that these differences are due to changes in several tissue processes that may contribute to improved cardiac healing after MI. Matrix-injected infarcted myocardium exhibits an altered inflammatory response, reduced cardiomyocyte apoptosis, enhanced infarct neovascularization, diminished cardiac hypertrophy and fibrosis, altered metabolic enzyme expression, increased cardiac transcription factor expression, and progenitor cell recruitment, along with improvements in global cardiac function and hemodynamics. Conclusions These results indicate that the myocardial matrix alters several key pathways after MI creating a pro-regenerative environment, further demonstrating its promise as a potential post-MI therapy

Bone marrow-derived cells can acquire cardiac stem cells properties in damaged heart

Experimental data suggest that cell-based therapies may be useful for cardiac regeneration following ischaemic heart disease. Bone marrow (BM) cells have been reported to contribute to tissue repair after myocardial infarction (MI) by a variety of humoural and cellular mechanisms. However, there is no direct evidence, so far, that BM cells can generate cardiac stem cells (CSCs). To investigate whether BM cells contribute to repopulate the Kit+ CSCs pool, we transplanted BM cells from transgenic mice, expressing green fluorescent protein under the control of Kit regulatory elements, into wild-type irradiated recipients. Following haematological reconstitution and MI, CSCs were cultured from cardiac explants to generate 'cardiospheres', a microtissue normally originating in vitro from CSCs. These were all green fluorescent (i.e. BM derived) and contained cells capable of initiating differentiation into cells expressing the cardiac marker Nkx2.5. These findings indicate that, at least in conditions of local acute cardiac damage, BM cells can home into the heart and give rise to cells that share properties of resident Kit+ CSCs

Magnetobiostratigraphy of the Spathian to Anisian (Lower to Middle Triassic) Kçira section, Albania

Magnetobiostratigraphic data are presented from three Early/Middle Triassic Han-Bulog Limestone successions from Kçira, northern Albania. A total of 206 standard palaeomagnetic samples were obtained for thermal demagnetization and statisticalanalysis from the 42, 10 and 5m thick sections. The reversal-bearing characteristic component, carried by haematite and magnetite, defines a composite sequence of six main polarity intervals (Kçln to Kç3r) in which are embedded four short polarity intervals, one at the base of Kçln and three towards the top of Kçlr. The early acquisition of the characteristic remanence is supported by the lateral correlation of magnetozones between sections. The Early/Middle Triassic boundary, approximated by the first occurrence of the conodont Chiosella timorensis, falls close to the Kçlr/Kç2n polarity transition. This is in good agreement with recently published magnetobiostratigraphic data from the coeval Chios (Greece) sections. The palaeomagnetic pole calculated from the Kçira characteristic directions lies close to the Triassic portion of the apparent polar wander path for Laurussia (in European coordinates). However, a 40-45" clockwise rotation of the external zone of the Albano-Hellenic Belt to the south of the Scutari-Pec Line is thought to have occurred since the Early-Middle Miocene. The Kçira pole acquires a West Gondwana affinity when restored for the Neogene clockwise rotation. If the clockwise rotation was entirely related to Neogene tectonics, the Kçira area was evidently associated with West Gondwana and located at 12-16"N of the western Tethys margin

Towards a better definition of the Middle Triassic magnetostratigraphy and biostratigraphy in the Tethyan realm

Magnetostratigraphic and biostratigraphic data for the Middle Triassic (Anisian) were obtained from the Han-Bulog facies in the Nderlysaj section from the Albanian Alps and the Dont and Bivera formations in the Dont–Monte Rite composite section from the Dolomites region of northern Italy. The Nderlysaj section is biochronologically bracketed between the late Bithynian and early Illyrian substages (i.e., late-early and early-late Anisian), whereas the Dont–Monte Rite section comprises the late Pelsonian and the early Illyrian substages. The data from Nderlysaj and Dont–Monte Rite, in conjunction with already published data, allow us to construct a nearly complete composite geomagnetic polarity sequence tied to Tethyan ammonoid and conodont biostratigraphy from the late Olenekian (late-Early Triassic) to the late Ladinian (late-Middle Triassic). New conodont data require revision of the published age of the Vlichos section (Greece)

Acercándose a la investigación

Nota de prensa en la que trata de impulsar el interés por la investigación. El Instituto de Salud Carlos III colabora en el programa “INVESTIGA I+D+i”, puesto en marcha por la Fundación San Patricio, para promover el interés de los adolescentes por la cienci

ACTH-dependent Cushing's Syndrome: Diagnostic Pitfalls in Concomitant Non-secreting Pituitary Adenomas

Objectives: To describe the possible pitfalls in correctly interpreting clinical, radiological and biochemical findings in ACTH-dependent Cushing's syndrome. Methods: We describe a case of a pituitary adenoma visualized at MRI not correlated with an ACTH-dependent Cushing’s syndrome. Results: Radiological imaging and hormonal testing can be misleading in suspected pituitary ACTH-related Cushing’s syndrome. Conclusion: Correct interpretation of the initial clinical presentation can help in the proper diagnosis and treatment of ACTH-dependent Cushing’s syndrome