Experiências de contacto dos bebés com a linguagem escrita

The purpose of this study is to characterize the literacy experiences ofbabies aged between 14 and 24 months. In this study, four issues were analysed: which type of written language experiences are offered to the children, how parents value these literacy experiences, the way parents perceive their own role in the literacy development of their children and what parents know about their means of contributing to the literacy development of their children. Six mothers were interviewed. These interviews were analysed with the use of a categorical content analysis. The results suggest that mothers value and include in their practices facilitative and mediation actions of literacy experiences with their children, noting some variability depending on the respective qualifications.Este estudo exploratório visa descrever as experiências de literacia de bebés entre os 14 e os 25 meses. Analisamse quatro questões, que experiências envolvendo a linguagem escrita no seio familiar são proporcionadas aos bebés, qual o valor que os pais lhes atribuem, como percepcionam o seu papel na promoção do desenvolvimento literácito dos filhos e como podem contribuir para o desenvolvimento da literacia dos mesmos. Adotou-se uma metodologia qualitativa, com recurso à análise de conteúdo categorial das respostas dadas por seis mães em entrevista individual e semiestruturada. Concluiu-se que estas mães valorizam e incluem nas suas práticas ações facilitadoras e de mediação de experiências de literacia por parte dos seus bebés, notando-se alguma variabilidade em função das respetivas habilitações académicas.(undefined)info:eu-repo/semantics/publishedVersio

Calpain-Catalyzed Proteolysis of Human dUTPase Specifically Removes the Nuclear Localization Signal Peptide

Calpain proteases drive intracellular signal transduction via specific proteolysis of multiple substrates upon Ca(2+)-induced activation. Recently, dUTPase, an enzyme essential to maintain genomic integrity, was identified as a physiological calpain substrate in Drosophila cells. Here we investigate the potential structural/functional significance of calpain-activated proteolysis of human dUTPase.Limited proteolysis of human dUTPase by mammalian m-calpain was investigated in the presence and absence of cognate ligands of either calpain or dUTPase. Significant proteolysis was observed only in the presence of Ca(II) ions, inducing calpain action. The presence or absence of the dUTP-analogue α,β-imido-dUTP did not show any effect on Ca(2+)-calpain-induced cleavage of human dUTPase. The catalytic rate constant of dUTPase was unaffected by calpain cleavage. Gel electrophoretic analysis showed that Ca(2+)-calpain-induced cleavage of human dUTPase resulted in several distinctly observable dUTPase fragments. Mass spectrometric identification of the calpain-cleaved fragments identified three calpain cleavage sites (between residues (4)SE(5); (7)TP(8); and (31)LS(32)). The cleavage between the (31)LS(32) peptide bond specifically removes the flexible N-terminal nuclear localization signal, indispensable for cognate localization.Results argue for a mechanism where Ca(2+)-calpain may regulate nuclear availability and degradation of dUTPase

Recommendations for effective documentation in regional anesthesia: an expert panel Delphi consensus project

Background and objectives: Documentation is important for quality improvement, education, and research. There is currently a lack of recommendations regarding key aspects of documentation in regional anesthesia. The aim of this study was to establish recommendations for documentation in regional anesthesia. Methods: Following the formation of the executive committee and a directed literature review, a long list of potential documentation components was created. A modified Delphi process was then employed to achieve consensus amongst a group of international experts in regional anesthesia. This consisted of 2 rounds of anonymous electronic voting and a final virtual round table discussion with live polling on items not yet excluded or accepted from previous rounds. Progression or exclusion of potential components through the rounds was based on the achievement of strong consensus. Strong consensus was defined as ≥75% agreement and weak consensus as 50%-74% agreement. Results: Seventy-seven collaborators participated in both rounds 1 and 2, while 50 collaborators took part in round 3. In total, experts voted on 83 items and achieved a strong consensus on 51 items, weak consensus on 3 and rejected 29. Conclusion: By means of a modified Delphi process, we have established expert consensus on documentation in regional anesthesia