Cell viability %.

<p>The histograms show how changes the viability in MCF7 cell lines after MTZ, vit C, and their combination treatment for 48 h. Experiments were in triplicate. On the x-axis are showed the different drugs concentrations (DC): 1 (MTZ: 0.07 µM; vit C: 0.09 mM), 2 (MTZ: 0.15 µM; vit C: 0.19 mM), 3 (MTZ: 0.29 µM; vit C: 0.38 mM), 4 (MTZ: 0.58 µM; vit C: 0.75 mM), 5 (MTZ: 1.17 µM; vit C: 1.5 mM), 6 (MTZ: 2.34 µM; vit C: 3 mM), 7 (MTZ: 4.68; vit C: 6 mM), 8 (MTZ: 9.36; vit C: 12 mM) for MCF7 and 1(MTZ: 0.04 µM; vit C: 0.03 mM), 2 (MTZ: 0.75 µM; vit C: 0.06 mM), 3 (MTZ: 0.15 µM; vit C: 0.13 mM), 4 (MTZ: 0.3 µM; vit C: 0.25 mM), 5 (MTZ: 0,6 µM; vit C: 0.5 mM), and 6 (MTZ: 1,2 µM; vit C: 1 mM), 7 (MTZ:2,4; vit C: 2 mM), 8 (MTZ: 4,8; vit C: 4 mM) for MDA-MB231; on the y-axis: the viability percentage is presented as means ± standard deviation. Moreover, we indicate with “a” or “b” if the difference between cell viability after co-treatment and vit c or MTZ is statistically significant (with p-value<0.05).</p

Cell percentages in G0/G1, S and G2/M phases in MCF7 and in MDA-MB231 cells.

<p>Cell percentages in G0/G1, S and G2/M phases in MCF7 and in MDA-MB231 cells.</p

Percentage of live, early apoptotic, late apoptotic, and dead cells expressed as mean ±standard deviation by the Muse Annexin V and Dead Cell assay in MCF7 and in MDA-MB231 cells.

<p>Percentage of live, early apoptotic, late apoptotic, and dead cells expressed as mean ±standard deviation by the Muse Annexin V and Dead Cell assay in MCF7 and in MDA-MB231 cells.</p

MTZ and vit C co-treatment induced a synergistic anti-proliferative effect compared to treatment with drugs administered individually as demonstrated by median drug effect analysis calculating the combination index (CI) and the dose redaction index (DRI) with CalcuSyn software.

<p>“r” is the linear correlation coefficient.</p><p>MTZ and vit C co-treatment induced a synergistic anti-proliferative effect compared to treatment with drugs administered individually as demonstrated by median drug effect analysis calculating the combination index (CI) and the dose redaction index (DRI) with CalcuSyn software.</p

H2AX and PI3K expression activation percentages for each cell population (inactive, active and not-expressing) compared to the total cell population.

<p>We report mean values ± standard deviation and indicate with * (p-value<0.05) if the activation difference in treated and untreated cells is statistically significant.</p><p>H2AX and PI3K expression activation percentages for each cell population (inactive, active and not-expressing) compared to the total cell population.</p