Absolute Frequency Atlas from 915 nm to 985 nm based on Laser Absorption Spectroscopy of Iodine

This article reports on laser absorption spectroscopy of iodine gas between 915 nm and 985 nm. This wavelength range is scanned utilizing a narrow linewidth and mode-hop-free tunable diode-laser whose frequency is actively controlled using a calibrated wavelength meter. This allows us to provide an iodine atlas that contains almost 10,000 experimentally observed reference lines with an uncertainty of 50 MHz. For common lines, good agreement is found with a publication by Gerstenkorn et al. [S. Gerstenkorn, P. Luc, Atlas du spectre d'absorption de la molecule d'iode 14800-20000 cm$^{-1}$, Paris: Editions du Centre National de la Recherche Scientifique (CNRS), 1978]. The new rich dataset allows existing models of the iodine molecule to be refined and can serve as a reference for laser frequency calibration and stabilization.Comment: 7 pages, 4 figures, link to online data depositor

Additional file 2: of The refractive state of the eye in Icelandic horses with the Silver mutation

Tabular data generated during this study can be found in Additional file 2. (XLSX 20 kb

Additional file 1: of The refractive state of the eye in Icelandic horses with the Silver mutation

Medical record for the ophthalmic examinations (DOCX 12 kb

Additional file 13 of Genome data uncover four synergistic key regulators for extremely small body size in horses

Primers for validation of two homozygous deletions found in Shetland ponies. Multiplex-PCR reverse primers located in the deletion and proximal of the deletion were used for targeting the two deletions. Product sizes and annealing temperatures are displayed. (DOCX 12 kb

Additional file 11 of Genome data uncover four synergistic key regulators for extremely small body size in horses

General linear model analysis testing for genotypic and allelic effects on cannon bone circumference. The effect on cannon bone circumference is shown for all three polymorphisms genotyped in 52 Shetland ponies whose cannon bone circumference measurements were available. (DOCX 13 kb

Additional file 6 of Genome data uncover four synergistic key regulators for extremely small body size in horses

Graphical representation of CNV detection results. Plots of log2 ratios of comparative analysis of three Shetland ponies with warmblood control group (a), Thoroughbred control group (b), non-breed control group (c) or “other breeds” control group (d) are displayed. (TIFF 3829 kb

Additional file 12 of Genome data uncover four synergistic key regulators for extremely small body size in horses

Primers and assays for validation of variants in ROH regions. SNVs in ROH regions specifically filtered for their functional effects were genotyped using Kompetitive Allele Specific PCR (KASP). Primer sequences, annealing temperature and number of PCR cycles are shown. (DOCX 23 kb

Additional file 5 of Genome data uncover four synergistic key regulators for extremely small body size in horses

Functional classification of genes. Variants identified in ROH regions in the two Miniature Shetland ponies and one standard sized Shetland pony, which were estimated to be deleterious (SIFT prediction) were investigated for their genomic positions in genes. In addition, variants, which showed an exclusively homozygous mutant genotype in all three Shetland ponies, were added to the analysis. Human orthologue genes were functionally classified using DAVID Functional Annotation Tool. (XLSX 23 kb

Additional file 1 of Genome data uncover four synergistic key regulators for extremely small body size in horses

Mapping statistics of whole-genome sequencing data. In total 32 samples of equids of different populations were analyzed in this study. Sequence read achieve ID, sequencing parameters and mean coverage are shown. (DOCX 15 kb

Additional file 8 of Genome data uncover four synergistic key regulators for extremely small body size in horses

Intersection of potential Shetland pony-specific CNV regions (CNVRs). The overlap of four CNV detection results for Shetland pony-specific CNVRs shows 97 CNVR harboring 14 different genes. (DOCX 18 kb