Intratumoral heterogeneity and variation among different patients for the expression of 4 exemplary proteins (VEGF, VEGFR, EGFR and p1068EGFR) assessed by reverse phase protein arrays.

<p>Box plots show the median (line within the box), 25th and 75th percentiles, and whiskers are showing 1.5 times the interquartile range.</p

Comparison of intratumoral heterogeneity and variation among patients for all proteins, phosphorylated and non-phosphorylated proteins.

<p>CV, coefficient of variation.</p><p>Overall, all proteins combined; Phospho, all phosphorylated proteins combined; Non-phospho, all non-phosphorylated proteins combined.</p><p>Healthy tubes, fallopian tube epithelium from healthy individuals.</p><p>Contralateral tubes, morphologically normal contralateral fallopian tube epithelium from ovarian cancer patients.</p

Variation between 88 individual tumor samples from 13 patients assessed by non-supervised hierarchical clustering based on expression of 36 proteins.

<p>Different patients are color-coded as indicated in the figure legend. High relative expression of proteins is shown in red and low expression in green color. Grey spaces indicate missing data points.</p

Impact of sampling method on identification of proteins differentially expressed in tumors vs. normal tissues.

*<p>randomly selected single tumor samples per case; this analysis was repeated 3 times (tumor samples 1–3).</p><p>+ and − symbols after p-values indicate significant upregulation (+) or downregulation (−) of the respective protein in tumor vs. normal tissues.</p><p>n.s., not significant (>0.05).</p><p><i>Italics</i> indicate the group of proteins identified as differentially expressed by all four approaches.</p