TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells-5

<p><b>Copyright information:</b></p><p>Taken from "TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells"</p><p>http://www.biomedcentral.com/1471-2202/8/49</p><p>BMC Neuroscience 2007;8():49-49.</p><p>Published online 4 Jul 2007</p><p>PMCID:PMC1931605.</p><p></p> lysates of mock- (line 1), TRPM5- (line 2) and YFP-TRPM5- (line 3) transfected HEK 293 cells. Positions of TRPM5- and YFP-TRPM5-specific bands are indicated by arrowheads. () Overlay of confocal and corresponding differential interference contrast (DIC) images of immunofluorescence staining of HEK 293 cells transiently expressing wild-type TRPM5. () Immunohistochemistry of taste buds using a TRPM5-specific antibody at lower (C) and higher magnifications (D, E). Notably, TRPM5 is predominantly localized on the basolateral surface of cells (indicated by the arrow in (D, E)). () Lack of TRPM5 immunoreactivity in taste receptors after preincubation of the TRPM5-specific antibody with the immunization peptide. () Fluorescent labeling of taste buds with the TRPM5-specific antibody (E) and the UEA lectin (H) and overlay of both with corresponding DIC image (I). Note that only a subset of cells in the taste bud is enriched in TRPM5. In the TRPM5-positive cells, TRPM5 is mainly localized on the basolateral cell surface and absent in microvilli (as indicated by an arrow (G, H, I)). Scale bars are 20 μm each

TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells-1

<p><b>Copyright information:</b></p><p>Taken from "TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells"</p><p>http://www.biomedcentral.com/1471-2202/8/49</p><p>BMC Neuroscience 2007;8():49-49.</p><p>Published online 4 Jul 2007</p><p>PMCID:PMC1931605.</p><p></p> () Immunolocalization of TRPM5 in solitary cells of the main olfactory epithelium is shown at lower (indicated by the arrows in (A, B)) and higher magnifications (C, D). () Immunohistochemistry of TRPM5 in the VNO. (E, F) Localization of TRPM5 on the apical surface of sensory epithelia (indicated by the arrow). (G, H) Detection of TRPM5-enriched solitary cells in the non-sensory part of the VNO. Scale bars are 20 μm

TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells-3

<p><b>Copyright information:</b></p><p>Taken from "TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells"</p><p>http://www.biomedcentral.com/1471-2202/8/49</p><p>BMC Neuroscience 2007;8():49-49.</p><p>Published online 4 Jul 2007</p><p>PMCID:PMC1931605.</p><p></p>depicted. () TRPM5-expressing cells are abundant in the cardiac region of the stomach. In gut epithelia, TRPM5-enriched cells are detected in the duodenal villi (), the ileum () and the colon (). Scale bars are 20 μm

TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells-4

<p><b>Copyright information:</b></p><p>Taken from "TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells"</p><p>http://www.biomedcentral.com/1471-2202/8/49</p><p>BMC Neuroscience 2007;8():49-49.</p><p>Published online 4 Jul 2007</p><p>PMCID:PMC1931605.</p><p></p> corresponding DIC images (right panels) are shown. The TRPM5-enriched cells in gastric glands of mouse stomach (A, B) and in mouse a duodenal villus (C, D) reveal a long basolateral processes (indicated by the arrows). Within duodenal epithelia (C, D), the TRPM5-immunoreactive cell contains a protrusion extending beyond the epithelial luminal border (indicated by an arrowhead). () Co-localization of TRPM5 with CK18 in intestinal glands of the rat duodenum. Confocal images of TRPM5 and CK18 specific signals (E, F) and their overlay with corresponding DIC images (G) are depicted. Scale bars are 10 μm

TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells-0

<p><b>Copyright information:</b></p><p>Taken from "TRPM5, a taste-signaling transient receptor potential ion-channel, is a ubiquitous signaling component in chemosensory cells"</p><p>http://www.biomedcentral.com/1471-2202/8/49</p><p>BMC Neuroscience 2007;8():49-49.</p><p>Published online 4 Jul 2007</p><p>PMCID:PMC1931605.</p><p></p> lysates of mock- (line 1), TRPM5- (line 2) and YFP-TRPM5- (line 3) transfected HEK 293 cells. Positions of TRPM5- and YFP-TRPM5-specific bands are indicated by arrowheads. () Overlay of confocal and corresponding differential interference contrast (DIC) images of immunofluorescence staining of HEK 293 cells transiently expressing wild-type TRPM5. () Immunohistochemistry of taste buds using a TRPM5-specific antibody at lower (C) and higher magnifications (D, E). Notably, TRPM5 is predominantly localized on the basolateral surface of cells (indicated by the arrow in (D, E)). () Lack of TRPM5 immunoreactivity in taste receptors after preincubation of the TRPM5-specific antibody with the immunization peptide. () Fluorescent labeling of taste buds with the TRPM5-specific antibody (E) and the UEA lectin (H) and overlay of both with corresponding DIC image (I). Note that only a subset of cells in the taste bud is enriched in TRPM5. In the TRPM5-positive cells, TRPM5 is mainly localized on the basolateral cell surface and absent in microvilli (as indicated by an arrow (G, H, I)). Scale bars are 20 μm each