Perceived security of tenure and housing consolidation in informal settlements: case studies from urban Fiji

quatter or informal settlements are an ever growing feature of the Fijian urban landscape. This study investigates the nature of perceived security of tenure and housing consolidation in seven informal settlements across Fiji. Understanding of the security of tenure needs to move beyond a legal/illegal dichotomy and focus on perceived security of tenure, which accepts that a much wider continuum of land-use rights typically exists. Housing consolidation or �self-help� housing improvements are also linked to security of tenure but have not been sufficiently investigated through a perceived security-of-tenure framework, especially with respect to the use of customary land. The study finds that both perceived security of tenure and housing consolidation are greater than might be expected. Policy implications are discussed and interventions are encouraged that look to increase perceived security of tenure and housing consolidation in existing settlements

Dynamic Regulation at the Neuronal Plasma Membrane: Novel Endocytic Mechanisms Control Anesthetic-Activated Potassium Channels and Amphetamine-Sensitive Dopamine Transporters: A Dissertation

Endocytic trafficking dynamically regulates neuronal plasma membrane protein presentation and activity, and plays a central role in excitability and plasticity. Over the course of my dissertation research I investigated endocytic mechanisms regulating two neuronal membrane proteins: the anesthetic-activated potassium leak channel, KCNK3, as well as the psychostimulant-sensitive dopamine transporter (DAT). My results indicate that KCNK3 internalizes in response to Protein Kinase C (PKC) activation, using a novel pathway that requires the phosphoserine binding protein, 14-3-3β, and demonstrates for the first time regulated KCNK3 channel trafficking in neurons. Additionally, PKC-mediated KCNK3 trafficking requires a non-canonical endocytic motif, which is shared exclusively between KCNK3 and sodium-dependent neurotransmitter transporters, such as DAT. DAT trafficking studies in intact ex vivo adult striatal slices indicate that DAT endocytic trafficking has both dynamin-dependent and –independent components. Moreover, DAT segregates into two populations at the neuronal plasma membrane: trafficking-competent and -incompetent. Taken together, these results demonstrate that novel, non-classical endocytic mechanisms dynamically control the plasma membrane presentation of these two important neuronal proteins

Crowdsourcing Question-Answer Meaning Representations

We introduce Question-Answer Meaning Representations (QAMRs), which represent the predicate-argument structure of a sentence as a set of question-answer pairs. We also develop a crowdsourcing scheme to show that QAMRs can be labeled with very little training, and gather a dataset with over 5,000 sentences and 100,000 questions. A detailed qualitative analysis demonstrates that the crowd-generated question-answer pairs cover the vast majority of predicate-argument relationships in existing datasets (including PropBank, NomBank, QA-SRL, and AMR) along with many previously under-resourced ones, including implicit arguments and relations. The QAMR data and annotation code is made publicly available to enable future work on how best to model these complex phenomena.Comment: 8 pages, 6 figures, 2 table

The Latest Red River Rivalry: The Supreme Court\u27s Recent Decision Regarding the Red River Compact

On June 13, 2013, the United States Supreme Court issued a unanimous decision in a “Red River Rivalry” with much greater implications than the annual football game. In Tarrant Regional Water District v. Herrmann, the court sided entirely with Oklahoma in that state’s dispute with Texas over the allocation of Red River water. This decision will have considerable impact on Texas’ ability to meet its ever-growing water needs. Moreover, the decision could be consequential for other interstate water compacts and the states relying on the rivers and tributaries governed by those agreements

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation

Plasma membrane proteins are a large, diverse group of proteins comprised of receptors, ion channels, transporters and pumps. Activity of these proteins is responsible for a variety of key cellular events, including nutrient delivery, cellular excitability, and chemical signaling. Many plasma membrane proteins are dynamically regulated by endocytic trafficking, which modulates protein function by altering protein surface expression. The mechanisms that facilitate protein endocytosis are complex and are not fully understood for many membrane proteins. In order to fully understand the mechanisms that control the endocytic trafficking of a given protein, it is critical that the protein s endocytic rate be precisely measured. For many receptors, direct endocytic rate measurements are frequently achieved utilizing labeled receptor ligands. However, for many classes of membrane proteins, such as transporters, pumps and ion channels, there is no convenient ligand that can be used to measure the endocytic rate. In the present report, we describe a reversible biotinylation method that we employ to measure the dopamine transporter (DAT) endocytic rate. This method provides a straightforward approach to measuring internalization rates, and can be easily employed for trafficking studies of most membrane proteins