Role of Drug Repurposing in Cancer Treatment and Liposomal Approach of Drug Targeting

Cancer is the leading cause of death, and incidences are increasing significantly and patients suffering from it desperately need a complete cure from it. The science of using an already-invented drug that has been approved by the FDA for a new application is known as “drug repurposing.” Currently, scientists are drawn to drug repositioning science in order to investigate existing drugs for newer therapeutic uses and cancer treatment. Because of their unique ability to target cancer cells, recently repurposed drugs and the liposomal approach are effective in the treatment of cancer. Liposomes are nanovesicles that are drastically flexible, rapidly penetrate deeper layers of cells, and enhance intracellular uptake. More importantly, liposomes are biocompatible, biodegradable; entrap both hydrophobic and hydrophilic drugs. This chapter summarizes various approaches to drug repurposing, as well as drug repurposing methods, advantages and limitations of drug repurposing, and a liposomal approach to using repurposed drugs in cancer targeting. This chapter also summarizes liposomal structure, drug loading, and the mechanism of liposomes in targeted cancer treatment. The lipid-based liposomal approach is emerging as a powerful technique for improving drug solubility, bioavailability, reducing side effects, and improving the therapeutic efficacy of repurposed drugs for cancer treatment

Efeito da aplicação pré-colheita de cálcio na qualidade e no teor de nutrientes de manga 'Tommy Atkins' Quality and nutrient level of mango cv. 'Tommy Atkins' as affected by calcium application before harves

Com o objetivo de avaliar o efeito da aplicação pré-colheita de cálcio na qualidade e no teor de nutrientes de frutos de manga Tommy Atkins, foi realizado um experimento em um pomar comercial com dez anos de idade, localizado no município de Petrolina-PE. Foram avaliados sete tratamentos, sendo duas fontes comerciais de cálcio e três dosagens de cada fonte (5,8; 11,6 e 17,4 mmol L-1 de Ca na forma de Ca-quelatizado e 45,0; 90,0 e 135,0 mmol L-1 de Ca na forma de CaCl2.2H2O), além de um tratamento-controle. O delineamento experimental foi em blocos ao acaso, com quatro repetições. As aplicações dos tratamentos foram realizadas quinzenalmente, iniciadas quando os frutos apresentavam tamanho chumbinho (5 a 10 mm) e estenderam-se até duas semanas antes da colheita. Foram realizadas seis pulverizações de uma calda contendo os tratamentos, sendo que, em cada aplicação, foram fornecidos 12,5 L/planta de calda. Os frutos foram colhidos no estádio de maturação 2. Quarenta frutos, agrupados em lotes de dez, foram acondicionados em caixas de papelão com capacidade para 6 kg e submetidos ao armazenamento por 0; 20; 30 e 40 dias sob refrigeração (10.5&plusmn;1.0&deg;C e 90&plusmn;5% de UR). Depois de retirados da câmara fria, os frutos foram mantidos por cinco dias em sala de amadurecimento a 21&plusmn;1&deg;C e 60&plusmn;5% de UR. Foram determinados os teores de N, K, Ca e Mg na casca e na polpa dos frutos no lote equivalente ao tempo inicial de armazenamento e avaliada a incidência de colapso interno em todos os lotes. A aplicação de cálcio tanto na forma quelatizada quanto de sal solúvel aumentou as concentrações do nutriente na casca dos frutos. Aplicações de Ca-quelatizado mostraram-se eficientes em aumentar as concentrações de cálcio na polpa dos frutos, podendo contribuir para prevenir a ocorrência de colapso interno em curto período de armazenamento.<br>The effect of calcium on quality and nutrient level of mango cv. "Tommy Atkins" was studied when applied prior to harvest on a ten-year old orchard, in Petrolina, State of Pernambuco, Brazil. Three levels of two commercial calcium sources (5.8; 11.6 and 17.3 mmol L-1 of Ca as chelate Ca and 45.0; 90.0 and 135.0 mmol L-1 of Ca as the soluble salt CaCl2.2H2O), and a control treatment were evaluated, in a randomized complete block design with four replications. Calcium was applied every two weeks, starting at the fruit set stage (fruits 5 to 10 mm long) up to two weeks before harvest. Six sprayings of solutions containing the calcium treatments were applied providing 12.5 L of the solution/plant in each application. The fruits were harvested in the ripening stage 2. Forty fruits were grouped in four lots of ten, placed into cardboard boxes with capacity for 6 kg and stored for 0, 20, 30 and 40 days under refrigeration (10.5&plusmn;1.0&deg;C and 90&plusmn;5% RH). After removed from the cold chamber, the fruits were kept for five days in a ripening room at 21&plusmn;1&deg;C and 60&plusmn;5% RH. N, K, Ca and Mg contents in the flesh and skin were determined in the lot equivalent to the shortest time of storage and the incidence of internal breakdown was evaluated in all four lots. Calcium application in both chelate and soluble salt forms increased the nutrient contents in the skin. Applications of chelate Ca showed to be efficient in increasing the calcium contents in the fruit flesh, contributing to prevent the occurrence of internal breakdown in short storage periods

Equilíbrio nutricional e distúrbios fisiológicos em manga 'Tommy Atkins' Nutritional balance and physiological disorders in mango 'Tommy Atkins'

Com o objetivo de avaliar o efeito do equilíbrio nutricional sobre a incidência de distúrbios fisiológicos em manga 'Tommy Atkins' cultivada no Vale do São Francisco, realizou-se um ensaio com frutos coletados no estádio de maturação fisiológica, classificados em frutos sem sintomas e com sintomas de distúrbio fisiológico. Os frutos das duas classes foram separados em casca, polpa e caroço, e levados para secagem em estufa a 65 &deg;C. Este material foi submetido a mineralização para a determinação das concentrações de N, K, Ca, Mg e B. Antes da desidratação, uma parte da polpa foi separada para as determinações do teor de sólidos solúveis totais (SST) e da acidez total titulável (ATT). Os resultados permitem concluir que, tanto as concentrações elevadas de Ca e Mg, como as baixas relações N/Ca e K/Ca, tanto na polpa quanto na casca, foram eficientes na prevenção de distúrbios fisiológicos nos frutos de mangueira; a concentração de nutrientes obtida na casca pode refletir melhor a condição da fisiopatia do que a concentração dos nutrientes na polpa dos frutos; os valores de SST e a relação SST/ATT determinada nos frutos com sintomas foram muito mais elevados do que nos frutos sem sintomas, devido a uma sobrematuração desordenada dos tecidos da polpa.<br>With the objective of evaluating the effect of the nutritional balance on the incidence of physiological disorders in mango cv. Tommy Atkins, grown in São Francisco River Valley, a trial was carried out with fruits harvested at physiological ripening stage, classified as fruits without and with physiological disorder symptoms. From all of them, skin, flesh and pit were separated and dried in a stove at 65ºC. This material was mineralized in order to determine N, K, Ca, Mg and B contents. Before dehydration, part of the flesh was taken for estimation of the total soluble solids (TSS) and total titrable acidity (TTA). The results allow to conclude that: high concentrations of Ca and Mg , as well as low ratios N/Ca and K/Ca, both in the flesh and in the skin, were efficient to prevent physiological disorders in mango fruits; the nutrient concentration in the skin may show better the condition of physiological disorders than the nutrient concentration in fruit flesh; and the TSS values and TSS/TTA ratio in fruits with symptoms were much higher than in fruits without symptoms, due to over ripening of flesh tissues