Expression levels of the <i>B</i>. <i>horsfieldi</i> unigenes by FPKM analysis.

<p>(A) The perspective of general distribution. (B) The dispersing perspective. BF: <i>B</i>. <i>horsfieldi</i> females, BP: <i>B</i>. <i>horsfieldi</i> pupae, BL: <i>B</i>. <i>horsfieldi</i> larvae, BM: <i>B</i>. <i>horsfieldi</i> males.</p

Detailed information on the candidate olfactory genes of <i>B</i>. <i>horsfieldi</i>.

<p>Detailed information on the candidate olfactory genes of <i>B</i>. <i>horsfieldi</i>.</p

Histogram of the clusters of eukaryotic orthologous groups (KOG) classification of the <i>B</i>. <i>horsfieldi</i> unigenes.

<p>Histogram of the clusters of eukaryotic orthologous groups (KOG) classification of the <i>B</i>. <i>horsfieldi</i> unigenes.</p

Histogram of the gene ontology (GO) classification of the <i>B</i>. <i>horsfieldi</i> unigenes.

<p>Histogram of the gene ontology (GO) classification of the <i>B</i>. <i>horsfieldi</i> unigenes.</p

Neighbor-joining phylogenetic tree of <i>B</i>. <i>horsfieldi</i> odorant binding proteins (BhorOBPs).

<p>Values indicated at the nodes are bootstrap values based on 1000 replicates. Scale bar = 0.1. Bhor: <i>Batocera horsfieldi</i>; Tcas: <i>Tribolium castaneum</i>; Ityp: <i>Ips typographus</i>; Dpon: <i>Dendroctonus ponderosae</i>; Acor: <i>Anomala corpulenta</i>; Tmol: <i>Tenebrio molitor</i>; Malt: <i>Monochamus alternatus</i>; Pdiv: <i>Phyllopertha diversa</i>; Cmon: <i>Cryptolaemus montrouzieri</i>; Dhel: <i>Dasrarcus helophoroides</i>; Rfer: <i>Rhynchophorus ferrugineus</i>; Cbow: <i>Colaphellus bowringi</i>; Lory: <i>Lissorhoptrus oryzophilus</i>; Hobl: <i>Holotrichia oblita</i>; Agla: <i>Anoplophora glabripennis</i>; Hele: <i>Hylamorpha elegans</i>; Darm: <i>Dendroctonus armandi</i>; Hpar: <i>Holotrichia parallela</i>; Bpra: <i>Brachysternus prasinus</i>; Pjap: <i>Popillia japonica</i>; Eori: <i>Exomala orientalis</i>; Acup: <i>Anomala cuprea</i>; Asch: <i>Anomala schonfeldti</i>; Aoct: <i>Anomala octiescostata</i>; Aruf: <i>Anomala rufocuprea</i>; Cbuq: <i>Cyrtotrachelus buqueti</i>. The olfactory genes from different species are marked with different colors (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0192730#pone.0192730.s024" target="_blank">S24 Text</a>).</p

Unigenes annotated in different databases.

<p>Unigenes annotated in different databases.</p

Scattergram of simple sequence repeats (SSRs) detected in the <i>B</i>. <i>horsfieldi</i> unigene sequences.

<p>Scattergram of simple sequence repeats (SSRs) detected in the <i>B</i>. <i>horsfieldi</i> unigene sequences.</p

Sex-specific expressions of candidate olfactory genes of <i>B</i>. <i>horsfieldi</i> by RT-qPCR.

<p>The expression levels of male and female adults are indicated by red and green bars, respectively. Expression levels were calculated by the 2^−ΔΔCT method with three biological repeats. Asterisk shows a significant expression level (Chi-square test, <i>p</i><0.05). (A–J) Sex-specific differentially expressed genes between male and female adults. (A) <i>BhorOBP1</i>, (B) <i>BhorOBP2</i>, (C) <i>BhorOBP3</i>, (D) <i>BhorCSP1</i>, (E) <i>BhorCSP2</i>, (F) <i>BhorCSP3</i>, (G) <i>BhorOBPC1</i>, (H) <i>BhorOBPC2</i>, (I) <i>BhorOBPC3</i>, (J) <i>BhorOBPC4</i>.</p

Neighbor-joining phylogenetic tree of <i>B</i>. <i>horsfieldi</i> chemosensory proteins (BhorCSPs).

<p>Values indicated at the nodes are bootstrap values based on 1000 replicates. Scale bar = 0.1. Hpar: <i>Holotrichia parallela</i>; Tmol: <i>Tenebrio molitor</i>; Ityp: <i>Ips typographus</i>; Dpon: <i>Dendroctonus ponderosae</i>; Tcas: <i>Tribolium castaneum</i>; Acor: <i>Anomala corpulenta</i>; Bhor: <i>Batocera horsfieldi</i>; Gdau: <i>Galeruca daurica</i>; Paen: <i>Pyrrhalta aenescens</i>; Pmac: <i>Pyrrhalta maculicollis</i>; Dhel: <i>Dasrarcus helophoroides</i>; Cbow: <i>Colaphellus bowringi</i>; Malt: <i>Monochamus alternatus</i>; Rdom: <i>Rhyzopertha dominica</i>; Hobl: <i>Holotrichia oblita</i>; Lory: <i>Lissorhoptrus oryzophilus</i>; Rfer: <i>Rhynchophorus ferrugineus</i>. The olfactory genes from different species are marked with different colors (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0192730#pone.0192730.s025" target="_blank">S25 Text</a>).</p