2 research outputs found
ATP-induced morphological changes in supporting cells of the developing cochlea
The developing cochlea of mammals contains a large group of columnar-shaped cells, which together form a structure known as Kölliker’s organ. Prior to the onset of hearing, these inner supporting cells periodically release adenosine 5′-triphosphate (ATP), which activates purinergic receptors in surrounding supporting cells, inner hair cells and the dendrites of primary auditory neurons. Recent studies indicate that purinergic signaling between inner supporting cells and inner hair cells initiates bursts of action potentials in auditory nerve fibers before the onset of hearing. ATP also induces prominent effects in inner supporting cells, including an increase in membrane conductance, a rise in intracellular Ca2+, and dramatic changes in cell shape, although the importance of ATP signaling in non-sensory cells of the developing cochlea remains unknown. Here, we review current knowledge pertaining to purinergic signaling in supporting cells of Kölliker’s organ and focus on the mechanisms by which ATP induces changes in their morphology. We show that these changes in cell shape are preceded by increases in cytoplasmic Ca2+, and provide new evidence indicating that elevation of intracellular Ca2+ and IP3 are sufficient to initiate shape changes. In addition, we discuss the possibility that these ATP-mediated morphological changes reflect crenation following the activation of Ca2+-activated Cl− channels, and speculate about the possible functions of these changes in cell morphology for maturation of the cochlea
Citrus juices technology
Citrus fruits are widely grown throughout the world and contain various bioactive compounds with antioxidant activities including vitamin C, carotenoids, and phenolic compounds. These components are very important for human health and provide protection against harmful free radicals. Citrus fruits are mostly consumed as fresh fruits or fruit juices. To obtain high quality and safe citrus juice, certain critical points (oil extraction from peel, juice extraction, pulp removing, pasteurization, evaporation, and aseptic filling) need to be taken into consideration during citrus juice processing. Firstly, oil extraction from the peel is a necessary step to limit the level of peel oil components in the juice. Secondly, selected juice extraction techniques and process conditions are very important for the yield and total quality of the juice. Thirdly, the pulp removal is an important step to remove most of pectinmethylesterase (PME) and its heat resistance isoenzymes. Further inactivation of remaining PME enzymes and pathogenic or spoilage microorganisms is also obtained with the pasteurization step. Finally, equipment used for the juice production and the concentration conditions have various effects on the sensory properties of the citrus juices. As a result, minimal processing would be applied to citrus juices if the processing steps detailed above are optimized. Obtaining clarified citrus juices from the citruses which have lower carotenoid content including lemon and lime juice is a new trend these days. It is needed to be focused on enzymation (depectinization), clarification assistance agents, and filtration conditions during the clarified juices production. Citrus peel (flavedo) and layer of albedo are the main byproducts of the citrus juice industry. Citrus peel oil is obtained from flavedo layer which has a significant commercial value. Recently, promising nonthermal food preservation technologies were developed including pulsed electric fields (PEF), high pressure processing (HPP), and ultrasonication process (US). These technologies are highly appreciated for their ability to extend the shelf life of food products without the application of heat, thus also preserving the quality attributes such as sensory quality and nutritional value, as well as controlling the microbiological safety of food products. © 2014, Springer Science+Business Media New York