Manual for Polysulphone Dosimeters Characterisation, Handling and Application as Personal UV Exposure Devices

Abstract not availableJRC.I-Institute for Health and Consumer Protection (Ispra

Characterisation of Spectrophotometers Used for Spectral Solar Ultraviolet Radiation Measurements.

Abstract not availableJRC.I-Institute for Health and Consumer Protection (Ispra

Aerosol Optical Depth in the UVB and Visible from Brewer Spectrophotometer Direct Irradiance Measurements: 1991 to 2002.

Abstract not availableJRC.I-Institute for Health and Consumer Protection (Ispra

Direct Traceability of the Portable QASUME Irradiance Scale to the Primary Irradiance Standard of the PTB.

see attachedJRC.I.5-Physical and chemical exposure

Characterisation Report of the Travelling Standard B5503

Abstract not availableJRC.I-Institute for Health and Consumer Protection (Ispra

Intercomparison of Monochromatic Source Facilities for the Determination of the Relative Spectral Response of Erythemal Broadband Filter Radiometers

Abstract not availableJRC.I-Institute for Health and Consumer Protection (Ispra

Quality Assurance of Spectral Solar UV Measurements: Results from 25 UV Monitoring Sites in Europe, 2002 to 2004

see attachedJRC.I.5-Physical and chemical exposure

Quality Assurance of Spectral Ultraviolet Measurements in Europe through the Development of a Transportable Unit 8QASUME). Report of Site Visits round 2002.

Abstract not availableJRC.I-Institute for Health and Consumer Protection (Ispra

Quality Assurance of Reference Standards from Nine European Solar-Ultraviolet Monitoring Laboratories.

Abstract not availableJRC.H-Institute for environment and sustainability (Ispra

Sequence and functional differences in the ATPase domains of CHD3 and SNF2H promise potential for selective regulability and drugability

Chromatin remodelers use the energy of ATP hydrolysis to regulate chromatin dynamics. Their impact for development and disease requires strict enzymatic control. Here, we address the differential regulability of the ATPase domain of hSNF2H and hCHD3, exhibiting similar substrate affinities and enzymatic activities. Both enzymes are comparably strongly inhibited in their ATP hydrolysis activity by the competitive ATPase inhibitor ADP. However, the nucleosome remodeling activity of SNF2H is more strongly affected than that of CHD3. Beside ADP, also IP6 inhibits the nucleosome translocation of both enzymes to varying degrees, following a competitive inhibition mode at CHD3, but not at SNF2H. Our observations are further substantiated by mutating conserved Q‐ and K‐residues of ATPase domain motifs. The variants still bind both substrates and exhibit a wild‐type similar, basal ATP hydrolysis. Apart from three CHD3 variants, none of the variants can translocate nucleosomes, suggesting for the first time that the basal ATPase activity of CHD3 is sufficient for nucleosome remodeling. Together with the ADP data, our results propose a more efficient coupling of ATP hydrolysis and remodeling in CHD3. This aspect correlates with findings that CHD3 nucleosome translocation is visible at much lower ATP concentrations than SNF2H. We propose sequence differences between the ATPase domains of both enzymes as an explanation for the functional differences and suggest that aa interactions, including the conserved Q‐ and K‐residues distinctly regulate ATPase‐dependent functions of both proteins. Our data emphasize the benefits of remodeler ATPase domains for selective drugability and/or regulability of chromatin dynamics