Cropping System Stability Drives SOC Sequestration and Increases Saturation Deficit in Hot Arid Durum Wheat Cropping Systems

Stability analysis has been extensively employed in evaluating the genotype x environment interaction, but it can also be conveniently applied to cropping systems. This study applied stability analysis to a long-term experiment (1998–2016) conducted in an experimental farm in southern Italy. The experiment involved two standard semi-arid cropping systems (WW—durum wheat monocropping and WP—durum wheat–field pea rotation), with two nitrogen fertilization levels (60 kg ha−1—N60 and 120 kg ha−1—N120), along with an unfertilized control (N0). Soil organic carbon (SOC) was estimated in all treatments at the end of the experiment to investigate the potential for SOC sequestration and soil C saturation by analyzing the stability of all investigated cropping conditions in relation to SOC sequestration. The study also investigated the relationship between C input and SOC sequestration. SOC sequestration per year was higher in WW than in WP, confirming that including legumes in the rotation did not enhance the SOC content. Similarly, the positive impact of nitrogen application on biomass yield did not affect SOC accumulation. The stability analysis revealed that over the long term, WW exhibited greater stability than WP, suggesting that, in semi-arid environments, a more stable cropping system can enhance soil carbon sequestration, with nitrogen fertilization exerting a scarce influence. Segmented regression analysis identified two distinct patterns of SOC accumulation based on C input, separated by a definite threshold of C input (breakpoint) quantified at 14.7 Mg ha−1. It was found that the steady-state C level, achieved after the accumulation of this C input, was reached much faster in WW than in WP, in which it could only be inferred at 23 years. In conclusion, this study underscores the role of cropping system stability in driving SOC sequestration. Furthermore, it emphasizes the potential of stability analysis in assisting stakeholders in refining their strategies for different cropping conditions in dry and rainy environments

Validation of colorimetric assays for hydrogen peroxide, nitrate and nitrite ions in complex plasma-treated water solutions

AbstractLiquids treated with cold plasma emerged as 'redox drugs' in biomedicine, as sources of reactive oxygen and nitrogen species targeting cellular functions, including wound healing and cancer progression. The use of cell culture media as starting liquid, however, challenges the identification of plasma‐generated chemistry, limited by the presence of many reactive species and organic compounds. Available detection methods need, therefore, to be confirmed in these liquids to avoid inaccurate results. In this research, robustness, linearity, accuracy and specificity of three colorimetric assays are investigated to detect H2O2, NO2− and NO3−, predominant plasma‐induced products. The results clearly highlight the presence of some factors affecting the detection in cell culture media like high concentrations of chlorides found interfering with the detection of NO3− in the medium

The prognostic importance of chronic end-stage diseases in geriatric patients admitted to 163 Italian ICUs

BACKGROUND: The number of elderly patients undergoing major surgical interventions and then needing admission to intensive care unit (ICU) grows steadily. We investigated this issue in a cohort of 232,278 patients admitted in five years (2011-2015) to 163 Italian general ICUs. METHODS: Surgical patients older than 75 registered in the GiViTI MargheritaPROSAFE project were analyzed. The impact on hospital mortality of important chronic conditions (severe COPD, NYHA class IV, dementia, end-stage renal disease, cirrhosis with portal hypertension) was investigated with two prognostic models developed yearly on patients staying in the ICU less or more than 24 hours. RESULTS: 44,551 elderly patients (19.2%) underwent emergency (47.3%) or elective surgery (52.7%). At least one severe comorbidity was present in 14.6% of them, yielding a higher hospital mortality (32.4%, vs. 21.1% without severe comorbidity). In the models for patients staying in the ICU 24 hours or more, cirrhosis, NYHA class IV, and severe COPD were constant independent predictors of death (adjusted odds ratios [ORs] range 1.67-1.97, 1.54-1.91, and 1.34-1.50, respectively), while dementia was statistically significant in four out of five models (adjusted ORs 1.23-1.28). End-stage renal disease, instead, never resulted to be an independent prognostic factor. For patients staying in the ICU less than 24 hours, chronic comorbidities were only occasionally independent predictors of death. CONCLUSIONS: Our study confirms that elderly surgical patients represent a relevant part of all ICUs admissions. About one of seven bear at least one severe chronic comorbidity, that, excluding end-stage renal disease, are all strong independent predictors of hospital death

Plasma Treated Water Solutions in Cancer Treatments: The Contrasting Role of RNS

Abstract: Plasma Treated Water Solutions (PTWS) recently emerged as a novel tool for the generation of Reactive Oxygen and Nitrogen Species (ROS and RNS) in liquids. The presence of ROS with a strong oxidative power, like hydrogen peroxide (H2O2), has been proposed as the main effector for the cancer‐killing properties of PTWS. A protective role has been postulated for RNS, with nitric oxide (NO) being involved in the activation of antioxidant responses and cell survival. However, recent evidences proved that NO‐derivatives in proper mixtures with ROS in PTWS could enhance rather than reduce the selectivity of PTWS‐induced cancer cell death through the inhibition of specific antioxidant cancer defenses. In this paper we discuss the formation of RNS in different liquids with a Dielectric Barrier Discharge (DBD), to show that NO is absent in PTWS of complex composition like plasma treated (PT)‐cell culture media used for in vitro experiments, as well as its supposed protective role. Nitrite anions (NO2‐) instead, present in our PTWS, were found to improve the selective death of Saos2 cancer cells compared to EA.hy926 cells by decreasing the cytotoxic threshold of H2O2 to non‐toxic values for the endothelial cell line

Lipid-based systems loaded with PbS nanocrystals: near infrared emitting trackable nanovectors

Hydrophobic PbS nanocrystals (NCs) emitting in the near infrared spectral region were encapsulated in the core of micelles and in the bilayer of liposomes, respectively, to form polyethylene glycol (PEG)-grafted phospholipids. The phospholipid-based functionalization process of PbS NCs required the replacement of the pristine capping ligand at the NC surface with thiol molecules. The procedures carried out for two systems, micelles and liposomes, using PEG-modified phospholipids were carefully monitored by optical, morphological and structural investigations. The hydrodynamic diameter and the colloidal stability of both micelles and liposomes loaded with PbS NCs were evaluated using Dynamic Light Scattering (DLS) and z-potential experiments, and both were satisfactorily stable in physiological media. The cytotoxicity of the resulting PbS NC-loaded nanovectors was assessed by the in vitro investigation on Saos-2 cells, indicating that the toxicity of the PbS NC loaded liposomes was lower than that of the micelles with the same NC cargo, which is reasonable due to the different overall composition of the two prepared nanocarriers. Finally, the cellular uptake in the Saos-2 cells of both the NC containing systems was evaluated by means of confocal microscopy studies by exploiting a visible fluorescent phospholipid and demonstrating the ability of both luminescent nanovectors to be internalized. The obtained results show the great potential of the prepared emitting nanoprobes for imaging applications in the second biological window