Potential for Development and Limitations of Regional Business Environment

The article considers some questions of institutional support for the development of the regional business environment. In connection with constantly growing social liabilities of regional and municipal authorities, which results in the upsurge of risks of budget imbalance at respective levels, the research provides a foundation to the necessity of forming favorable business environment promoting enterprise activity in regions. The process of creating institutes of a region’s business environment is analyzed with applying a matrix institutional model of entrepreneurship. The article reveals the mechanism stipulating the emergence of specific peculiarities of institutional configurations of the regional business environment. A conclusion is drawn about the decisive role of the institutional configuration of the business environment in the existing differences in the levels of social and economic development of regions. The researchers put forward a hypothesis that supporting a favorable institutional configuration of a region’s business environment that stimulates enterprise activity in the area cannot last indefinitely as its development is of a cyclical nature. Simulation parameters for the formation and maintenance of a favorable institutional configuration of the business environment are given. With the aim of practical application of the suggested approach, the authors suggest a methodology allowing for regional authorities to estimate the expediency of favorable institutional configuration of business environment, and on this basis to formulate rules of play for business so that these rules could boost business activity and increase the contribution of entrepreneurship to the region’s social and economic development. The approach under consideration provides an opportunity to substantiate a theoretical and methodological framework for developing practical mechanisms of projecting and further long-term maintenance of favorable institutional configuration of business environment which will contribute to expanding the region’s business activity, and which is resistant to negative effects of the external environment.Work produced with the financial support of the grant FSBI “Russian Humanitarian Science Foundation” in the framework of scientific — research project “Interaction of authority and business organizations in the municipalities: through compromise to mutual loyalty» № 15-12-02008

Temperature-Dependent Effects of ATP on Smooth and Skeletal Muscles

ATP acting via different subtypes of P2X and P2Y receptors induces contractions or relaxation of mammalian smooth muscles, while in skeletal muscles, ATP can pre- and postsynaptically modulate effect of acetylcholine. It was shown that effects of ATP on both types of the muscle are significantly changed when the temperature shifts from physiological condition. For example, contractile responses of rodent urinary bladder and vas deferens mediated by P2X receptors are markedly increased with the decrease of the temperature. Similarly, in frog skeletal muscles, ATP-induced inhibition of acetylcholine release became more pronounced at low temperatures. In case of mammalian skeletal muscle, effect of temperature on ATP-induced responses depends on the type of muscle—slow and fast. In this chapter, we will discuss temperature-dependent effects of ATP on different muscle contractility and their possible mechanisms

Program for experimental investigations of the mechanisms for the emission of coherent radiation by relativistic electrons with energies of 10-500 MeV in oriented crystals

A program of experimental investigations of the mechanisms for the emission of coherent radiation by electrons in periodic structures is proposed. The program is aimed at developing sources of energy-tunable quasimonochromatic x radiation and y radiationyesBelgorod State Universit

Influence of Administrative Regulation on the Efficiency of Business Activities in the Region

The article discusses the theoretical issues of the formation of the administrative regulation mechanisms for business entities. The necessity of the further development of theoretical and methodological base and the application tools for the design of business environment is proved. This can stimulate the growth of business and investment activity in the Russian regions and municipalities. The authors identify two types of government structures influence on the business entities, differentiated by the nature of the targeting impact on the economic activity of business structures—the administrative pressure and administrative assistance. It is suggested that in practice, high cost implications for compliance with all regulation requirements as well as sanctions for the violation of these requirements create preconditions for the development of informal interaction between entrepreneurs and the representatives of regulatory bodies. Therefore, businessmen try to minimize the costs associated with the implementation of formal administrative requirements, rules and regulations by personal arrangements. A mathematical model for the assessment of the informal interaction between business entities and certain officials of control supervisory authorities is proposed. It allows to determine the range of benefits for economic entities from avoiding the implementation of administrative norms, requirements and rules. It is concluded that unreasonably high level of costs for the implementation of formal administrative requirements rules and regulations by business entities composes the economic basis for the reproduction of informal relations. This determines mutual benefits for a number of entrepreneurs and a part of bureaucracy from various schemes of informal interaction.The article has been prepared with the support of the Grant of the Russian Foundation for Humanities in the framework of the research and development project " Development of a favourable institutional configuration of the regional business environment as the factor to increase the competitiveness of business entities" No. 16–12–02007

Identification of novel restriction endonuclease-like fold families among hypothetical proteins

Restriction endonucleases and other nucleic acid cleaving enzymes form a large and extremely diverse superfamily that display little sequence similarity despite retaining a common core fold responsible for cleavage. The lack of significant sequence similarity between protein families makes homology inference a challenging task and hinders new family identification with traditional sequence-based approaches. Using the consensus fold recognition method Meta-BASIC that combines sequence profiles with predicted protein secondary structure, we identify nine new restriction endonuclease-like fold families among previously uncharacterized proteins and predict these proteins to cleave nucleic acid substrates. Application of transitive searches combined with gene neighborhood analysis allow us to confidently link these unknown families to a number of known restriction endonuclease-like structures and thus assign folds to the uncharacterized proteins. Finally, our method identifies a novel restriction endonuclease-like domain in the C-terminus of RecC that is not detected with structure-based searches of the existing PDB database

Realm of PD-(D/E)XK nuclease superfamily revisited: detection of novel families with modified transitive meta profile searches

<p>Abstract</p> <p>Background</p> <p>PD-(D/E)XK nucleases constitute a large and highly diverse superfamily of enzymes that display little sequence similarity despite retaining a common core fold and a few critical active site residues. This makes identification of new PD-(D/E)XK nuclease families a challenging task as they usually escape detection with standard sequence-based methods. We developed a modified transitive meta profile search approach and to consider the structural diversity of PD-(D/E)XK nuclease fold more thoroughly we analyzed also lower than threshold Meta-BASIC hits to select potentially correct predictions placed among unreliable or incorrect ones.</p> <p>Results</p> <p>Application of a modified transitive Meta-BASIC searches on updated PFAM families and PDB structures resulted in detection of five new PD-(D/E)XK nuclease families encompassing hundreds of so far uncharacterized and poorly annotated proteins. These include four families catalogued in PFAM database as domains of unknown function (DUF506, DUF524, DUF1626 and DUF1703) and YhgA-like family of putative transposases. Three of these families represent extremely distant homologs (DUF506, DUF524, and YhgA-like), while two are newly defined in updated database (DUF1626 and DUF1703). In addition, we also confidently identified an extended AAA-ATPase domain in the N-terminal region of DUF1703 family proteins.</p> <p>Conclusion</p> <p>Obtained results suggest that detailed analysis of below threshold Meta-BASIC hits may push limits further for distant homology detection in the 'midnight zone' of homology. All identified families conserve the core evolutionary fold, secondary structure and hydrophobic patterns common to existing PD-(D/E)XK nucleases and maintain critical active site motifs that contribute to nucleic acid cleavage. Further experimental investigations should address the predicted activity and clarify potential substrates providing further insight into detailed biological role of these newly detected nucleases.</p

SCOPmap: Automated assignment of protein structures to evolutionary superfamilies

BACKGROUND: Inference of remote homology between proteins is very challenging and remains a prerogative of an expert. Thus a significant drawback to the use of evolutionary-based protein structure classifications is the difficulty in assigning new proteins to unique positions in the classification scheme with automatic methods. To address this issue, we have developed an algorithm to map protein domains to an existing structural classification scheme and have applied it to the SCOP database. RESULTS: The general strategy employed by this algorithm is to combine the results of several existing sequence and structure comparison tools applied to a query protein of known structure in order to find the homologs already classified in SCOP database and thus determine classification assignments. The algorithm is able to map domains within newly solved structures to the appropriate SCOP superfamily level with ~95% accuracy. Examples of correctly mapped remote homologs are discussed. The algorithm is also capable of identifying potential evolutionary relationships not specified in the SCOP database, thus helping to make it better. The strategy of the mapping algorithm is not limited to SCOP and can be applied to any other evolutionary-based classification scheme as well. SCOPmap is available for download. CONCLUSION: The SCOPmap program is useful for assigning domains in newly solved structures to appropriate superfamilies and for identifying evolutionary links between different superfamilies