15 research outputs found
Increasing the competitiveness of not large enterprises of marine transport owing to cooperation
В статті було досліджено взаємодію підприємств як спосіб підвищення рівня конкурентоздатності невеликого підприємств морської галузі України та проаналізовано основні форми взаємодії, що сприяють досягненню даної мети
Developing an operational radiographic inspection method for the elbow-shaped branch pipes at the NPP working with RBMK-1000
Preface Status of Metallic Materials Development for Application in Advanced High-Temperature Gas-Cooled Reactors
Electrophysiological characteristics of telocytes of the atrioventricular note and sinoatrial node perifocal area in humans and pigs
Little is known about the electrophysiological characteristics of telocytes found in the working myocardium and sinoatrial node (SAN). Telocyte expres -sion of HCN4 suggests the ability to generate pacemaker potentials. To prove the impulse conduction, the presence of voltage-gated sodium channels is required. It is assumed that telocytes are also located in the atrioventricular node (AVN).Aim. Morphological and electrophysiological study of AVN and SAN telocytes.Material and methods. Fragments of the right atrium, AVN of 7 hearts of recipients and 3 hearts of pigs were taken, respectively, during heart transplantation and after the experiment. Isolation of telocyte cultures, histological, immunohis tochemical tests with anti-CD117, anti-NaV1.5 (SCN5A), anti-CD34 antibodies, intravital confocal laser microscopy were carried out. The patch-clamp technique was used.Results. In AVN cultures, CD117+ cells with long processes were found. There was a potassium current with a density of 700 pA/pF during membrane depolarization up to +90 mW in humans and pigs using the patch-clamp technique. Calcium oscillations with a period of about 200 seconds in a pig with an increase in calcium concentration. In elongated cells located between cardiomyocytes and among the fibrous tissue of SAN perifocal zone, the co-expression of anti-NaV1.5 and anti-CD34 antibodies was revealed.Conclusion. In AVN, telocytes were found, in whose cultures potassium current and calcium oscillations were determined. SCN5A sodium channels were found in telocytes of the perifocal area of human SAN. This fact indicates the ability of cells to conduct an electrical impulse