17 research outputs found

    Range extensions along western Atlantic for Epialtidae crabs (Brachyura, Majoidea) genera Acanthonyx Latreille, 1828 and Epialtus H. Milne Edwards, 1834

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    The present study provided information extending the known geographical distribution of three species of majoid crabs, the epialtids Acanthonyx dissimulatus Coelho, 1993, Epialtus bituberculatus H. Milne Edwards, 1834, and E. brasiliensis Dana, 1852. Specimens of both genera from different carcinological collections were studied by comparing morphological characters. We provide new data that extends the geographical distributions of E. bituberculatus to the coast of the states of ParanĂĄ and Santa Catarina (Brazil), and offer new records from Belize and Costa Rica. Epialtus brasiliensis is recorded for the first time in the state of Rio Grande do Sul (Brazil), and A. dissimulatus is reported from Quintana Roo, Mexico. The distribution of A. dissimulatus, previously known as endemic to Brazil, has a gap between the states of EspĂ­rito Santo and Rio de Janeiro. However, this restricted southern distribution is herein amplified by the Mexican specimens

    ObservaçÔes sobre a ocorrĂȘncia de Pilumnus floridanus Stimpson (Decapoda, Brachyura, Pilumnidae) na costa do Rio de Janeiro, Brasil Remarks about the Pilumnus floridanus Stimpson (Decapoda, Brachyura, Pilumnidae) occurrence in Rio de Janeiro coast, Brazil

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    Os espécimes foram coletados na Baía de Sepetiba, Rio de Janeiro no inverno. Sua distribuição era conhecida para Off Cape Lookout, Carolina do Norte até a Flórida, Golfo do México, Canal de Yucatan, Honduras, Antilhas, Venezuela e Brasil (Alagoas, Sergipe e Bahia).<br>The specimens were collected in Sepetiba Bay during the winter. Their distribution was formerly known from Off Cape Lookout, North Carolina until Florida, Gulf of Mexico, Yucatan Channel, Honduras, Antilles, Venezuela and Brazil (Alagoas, Sergipe and Bahia)

    Feeding habits of the spider crab Libinia spinosa H. Milne Edwards, 1834 (Decapoda, Brachyura) in Ubatuba bay, SĂŁo Paulo, Brazil

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    The main goal of this study was the identification of the items of the diet of the L. spinosa, based on the stomach contents analysis. The crabs were obtained from Ubatuba region north-eastern shore of SĂŁo Paulo State. In the laboratory, all the individuals were dissected, the stomach was retreated and fixed in 10% formaline. The alimentary items were identified under stereomicroscope and analysed by the method of Frequency of Occurrence. A total of 194 stomachs was analysed and nine alimentary items were obtained. Unindentified material was found in 98% of analysed stomach and poriferan were present in less then 1% of stomachs. These results pointed a diversified diet explored by this crab, as well as the employment of some different methods for food intake. This suggested that these crabs could occupy different position in the trophic chain.<br>O objetivo deste trabalho Ă© identificar os itens alimentares que compĂ”em a dieta de L. spinosa, por meio da anĂĄlise do conteĂșdo estomacal. Os caranguejos foram coletados no litoral norte paulista (23Âș25’S-45Âș00’W). No laboratĂłrio, todos indivĂ­duos foram dissecados, os estĂŽmagos foram retirados e fixados em formol 10%. Os itens alimentares foram identificados sob estereomicroscĂłpio, sendo que para a anĂĄlise foi utilizado o mĂ©todo FreqĂŒĂȘncia de OcorrĂȘncia. Foram analisados 194 estĂŽmagos, nos quais foram encontrados nove itens alimentares. A maior freqĂŒĂȘncia foi para material nĂŁo identificado, ocorrendo em 98% dos estĂŽmagos analisados e Porifera foi o item com a menor freqĂŒĂȘncia, ocorrendo em menos de 1% dos estĂŽmagos. Com base nos resultados obtidos, sugere-se que o caranguejo L. spinosa apresenta uma dieta diversificada, sugerindo a utilização de diferentes mĂ©todos para a obtenção de alimento e por conseqĂŒĂȘncia podendo ocupar vĂĄrios nĂ­veis na cadeia trĂłfica

    Comunidade bacteriana como indicadora do efeito de feijoeiro geneticamente modificado sobre organismos nĂŁo alvo Bacterial community as an indicator of genetically modified common bean effect on nontarget organisms

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    O objetivo deste trabalho foi avaliar o efeito do feijoeiro geneticamente modificado quanto Ă  resistĂȘncia ao Bean Golden Mosaic VĂ­rus, BGMV (Olathe M1-4), sobre organismos nĂŁo alvo. De um experimento implantado no campo, em delineamento inteiramente casualizado, com dois tratamentos (Olathe Pinto e evento elite Olathe M1-4), dois perĂ­odos amostrais (estĂĄdio V4 e R6) e dez repetiçÔes, obtiveram-se cĂ©lulas bacterianas cultivadas e nĂŁo cultivadas da rizosfera e do solo nĂŁo rizosfĂ©rico, para as quais se procedeu Ă  extração de DNA total. A regiĂŁo V6-V8 do 16S rDNA foi amplificada para a comunidade bacteriana total, e tambĂ©m realizou-se amplificação com iniciadores especĂ­ficos para o subgrupo alfa (&#945;) do filo Proteobacteria a partir de cĂ©lulas nĂŁo cultivadas. Foram obtidos dendrogramas comparativos entre a variedade Olathe Pinto (convencional) e o evento elite Olathe M1-4 (geneticamente modificado) utilizando-se o coeficiente de Jaccard e o mĂ©todo UPGMA (Unweighted pair-group method with arithmetic mean). Os agrupamentos obtidos dos perfis de 16S rDNA PCR-DGGE indicam alteraçÔes na comunidade bacteriana da rizosfera em função da transformação das plantas sĂŁo mais notĂĄveis nos perfis obtidos para alfa-proteobacteria. A origem das amostras e o estĂĄgio de desenvolvimento das plantas afetam a comunidade bacteriana.<br>The objective of this work was to evaluate the effect of genetically modified common bean for Bean Golden Mosaic Virus, BGMV, resistance (Olathe M1-4) on nontarget organisms. In a field experiment established in a completely randomized design with two treatments (Olathe Pinto cultivar and M1-4 Olathe elite event), two sampling periods (V4 and R6 stages) and ten replicates, cultivated and non-cultivated bacterial cells from rhizosphere soil and bulk soil were obtained, and their total DNA was extracted. The V6-V8 region of 16S rDNA was amplified for the whole bacterial community, and primers specific for the alpha (&#945;) subgroup of the Proteobacteria phylum were obtained from uncultured cells and used for amplification. Using the Jaccard coefficient and UPGMA (Unweighted pair-group method with arithmetic mean), dendrograms comparing the conventional Olathe Pinto and the elite event Olathe M1-4 transgenic varieties were obtained. The clusters obtained from the 16S rDNA PCR-DGGE profiles indicate changes in the rhizosphere bacterial community in genetically modified plants, being more notable in the profiles obtained for alphaproteobacteria. Sample origin and plant development stages affect bacterial community profiles
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