A Study on understanding the use of a process color-based color communication systems to print synthetic colors accurately and consistently

The study is intended to provide a better understanding on the use of process color-based color communication systems such as those provided by Focoltone, Trumatch, and Pantone, in order to print synthetic colors as accurately and as consistently as possible. Using Focoltone\u27s system, an investigation was done on the materials and documentation, as provided by the Focoltone kit. The available information, as provided by Focoltone, was supplemented by the author\u27s interpretation of Focoltone\u27s specifications. From the author\u27s understanding of Focoltone specifications, a set of procedures were outlined that would contribute towards the accurate and consistent reproduction of synthetic colors on a press run. The study concludes that the use of standard film dots, as specified by Focoltone, to reproduce the reference colors on the swatchbook, is question able because the dot gain characteristics of press run(s) used to print Focoltone\u27s swatchbooks differ significantly from that of industry average values obtained from FIPP. Moreover, as each production press would exhibit its own dot gain characteristics, the use of a standard film dot in the printing process would not contribute to an accurate reproduction of color. Instead, the study recommends that procedures have to be established that: 1. Enable the conversion of standard film dot area specifications into custom film dot specifications to suit the particular press run\u27s dot gain characteristics. This procedure requires the availability of the plate/press curve of the printing process used to print the swatchbook, and that of the production press, as established in a fingerprint press run. 2. Ensure that the graphic reproduction process, from film to plate to press, is implemented in standardized conditions and manner, and that process control of SID be established, so that deviations from desired SID and dot gain are minimized. This way, the synthetic colors, using process color-based color communication systems, can be as printed as accurately, with reference to the reference color swatches, and as consistently throughout the press run

Sharp Garding inequality on compact Lie groups

In this paper the sharp Garding inequality is established on compact Lie groups. The positivity condition is expressed in the non-commutative phase space in terms of the full symbol, which is defined using the representations of the group. Applications are given to the $L^2$ and Sobolev boundedness of pseudo-differential operators.Comment: 18 page

Bridging of double-stranded breaks by the nonhomologous end-joining ligation complex is modulated by DNA end chemistry

The nonhomologous end-joining (NHEJ) pathway is the primary repair pathway for DNA double strand breaks (DSBs) in humans. Repair is mediated by a core complex of NHEJ factors that includes a ligase (DNA Ligase IV; L4) that relies on juxtaposition of 3΄ hydroxyl and 5΄ phosphate termini of the strand breaks for catalysis. However, chromosome breaks arising from biological sources often have different end chemistries, and how these different end chemistries impact the way in which the core complex directs the necessary transitions from end pairing to ligation is not known. Here, using single-molecule FRET (smFRET), we show that prior to ligation, differences in end chemistry strongly modulate the bridging of broken ends by the NHEJ core complex. In particular, the 5΄ phosphate group is a recognition element for L4 and is critical for the ability of NHEJ factors to promote stable pairing of ends. Moreover, other chemical incompatibilities, including products of aborted ligation, are sufficient to disrupt end pairing. Based on these observations, we propose a mechanism for iterative repair of DSBs by NHEJ

DNA Ligase IV Guides End-Processing Choice during Nonhomologous End Joining

Nonhomologous end joining (NHEJ) must adapt to diverse end structures during repair of chromosome breaks. Here, we investigate the mechanistic basis for this flexibility. DNA ends are aligned in a paired-end complex (PEC) by Ku, XLF, XRCC4, and DNA ligase IV (LIG4); we show by single-molecule analysis how terminal mispairs lead to mobilization of ends within PECs and consequent sampling of more end-alignment configurations. This remodeling is essential for direct ligation of damaged and mispaired ends during cellular NHEJ, since remodeling and ligation of such ends both require a LIG4-specific structural motif, insert1. Insert1 is also required for PEC remodeling that enables nucleolytic processing when end structures block direct ligation. Accordingly, cells expressing LIG4 lacking insert1 are sensitive to ionizing radiation. Cellular NHEJ of diverse ends thus identifies the steps necessary for repair through LIG4-mediated sensing of differences in end structure and consequent dynamic remodeling of aligned ends

Wound Healing in Peripheral Arterial Disease: Current and Future Therapy

Wounds secondary to peripheral arterial disease (PAD) result in substantial morbidity and burden to the healthcare industry. To aid in the care of this patient population, knowledge of the disease process and current standards of therapy is paramount for healthcare providers.The future care of these patients and improvement from our existing standards hinges on the active translational research. To implement new technologies and advances in the treatment of PAD-induced wounds and ensure adequate utilization of our current therapies a PAD-wound team is necessary

Vaping: Exploring perceptions and behaviors of students and ENDS products

Background: Electronic nicotine delivery systems (ENDS) have increased in use and media exposure with a 78% rise in usage between 2017 to 2018. While a gateway to addictive products, users are also unaware of the ingredients and their effects on the body. Interventions are limited. Further research is needed to investigate perceptions of ENDS and identify effective cessation strategies for young adults. Purpose: To explore the knowledge and attitudes of HS students regarding ENDS products and compare data collected over the year to determine effects of recent media exposure. Methods: Six focus groups (n = 45) were held in a rural high school. Students were asked four open ended questions which elicited dialogue regarding attitudes and behaviors about ENDS and suggestions for educational interventions. Results: Emerging themes were social and behavioral influences (peer pressure; nicotine addiction) ; knowledge deficit; media promotion or prevention. Suggested educational methods were explanation of the ingredients in ENDS, dramatic relief and visual depiction of the consequences. There was more focus in the media on prevention than last year. Conclusion/Implications: A knowledge deficit regarding the dangers of ENDS, including the damaging effects of nicotine, still exists. Data will lead to development of innovative interventions to decrease ENDS use in high schoolers.https://orb.binghamton.edu/research_days_posters_spring2020/1089/thumbnail.jp

Analysis of 6,515 exomes reveals a recent origin of most human protein-coding variants

Establishing the age of each mutation segregating in contemporary human populations is important to fully understand our evolutionary history1,2 and will help facilitate the development of new approaches for disease gene discovery3. Large-scale surveys of human genetic variation have reported signatures of recent explosive population growth4-6, notable for an excess of rare genetic variants, qualitatively suggesting that many mutations arose recently. To more quantitatively assess the distribution of mutation ages, we resequenced 15,336 genes in 6,515 individuals of European (n=4,298) and African (n=2,217) American ancestry and inferred the age of 1,146,401 autosomal single nucleotide variants (SNVs). We estimate that ~73% of all protein-coding SNVs and ~86% of SNVs predicted to be deleterious arose in the past 5,000-10,000 years. The average age of deleterious SNVs varied significantly across molecular pathways, and disease genes contained a significantly higher proportion of recently arisen deleterious SNVs compared to other genes. Furthermore, European Americans had an excess of deleterious variants in essential and Mendelian disease genes compared to African Americans, consistent with weaker purifying selection due to the out-of-Africa dispersal. Our results better delimit the historical details of human protein-coding variation, illustrate the profound effect recent human history has had on the burden of deleterious SNVs segregating in contemporary populations, and provides important practical information that can be used to prioritize variants in disease gene discovery