On-line Process For Pressurized Ethanol Extraction Of Onion Peels Extract And Particle Formation Using Supercritical Antisolvent

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)This paper brings forward a novel process for particle production named extraction and particle formation on-line (EPFO). The process comprises the hyphenated on-line association of pressurized liquid extraction (PLE) with supercritical antisolvent (SAS) precipitation performed in the same multipurpose equipment. The highlight of EPFO process stands for using the pressure energy applied in the extraction step as a useful energy for spraying and producing microparticles in the precipitation step. Performing EPFO process also prevents degrading bioactive compounds because the extract directly flows to the precipitation vessel, avoiding light and oxygen exposure. Then, we obtained dried microparticles as a fine powder from onion peels. After studying some different extraction conditions using ethanol, the more suitable one was found: 40 degrees C/120 bar for extraction step and 40 degrees C/100 bar for precipitation step. In such condition, we obtained 4.1 +/- 0.6 g microparticles/100 g of dried onion peels, the highest quercetin content in the microparticles (26 +/- 2 wt.%) and the smallest mean particle diameter (d([3,4])=119 +/- 1 mu m). Analysis of morphology showed lengthened microparticles with rod-like structures. We presented the microparticles characteristics obtained between EPFO process and conventional process (rotary evaporation), whereas EPFO process produced smaller microparticles more concentrated with quercetin. (C) 2015 Elsevier B.V. All rights reserved.110230239CAPES (DEA/FEA/PROEX)CNPq [472023/2006-3, 301301/2010-7]FAPESP [2012/10685-8, 2015/13299-0]Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Replacing Modified Starch By Inulin As Prebiotic Encapsulant Matrix Of Lipophilic Bioactive Compounds

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)The purpose of this work was to replace modified starch (SF) by inulin (IN), a prebiotic carbohydrate, during emulsification assisted by ultrasound. Oregano extract was encapsulated using five proportions of IN and SF as wall materials. The effect of such substitution on the microparticle characteristics was evaluated. Attempting to contribute with the increasing demand for prebiotic consumption, mixing one part of SF with three parts of IN (1:3, mass basis) yielded encapsulation efficiency equal to 66 +/- 1% and the largest thymol retention: 84 +/- 9%. Besides the entrapment of thymol, high amount of other compounds present in oregano extract could be entrapped in the polymeric matrix: 92 +/- 1%. Reduction of the micropartides sizes when increasing the proportion of inulin was also observed. Comprising such results and those presented for powder morphology, surface extract, particle size distribution, X-ray diffraction and thermal stability, the proportion 1:3 (SF:3IN) is a favorable prebiotic encapsulant matrix for encapsulating oregano extract and retaining target bioactive compounds. (C) 2016 Elsevier Ltd. All rights reserved.852635CNPq [470916/2012-5, 140275/2014-2, 301301/2010-7]FAPESP [2012/10685-8, 2015/13299-0, 2014/15685-1, 2015/22226-6]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

A detailed design and construction of a supercritical antisolvent precipitation equipment

This work consists of the steps for the assembly of a Supercritical Antisolvent Precipitation laboratory equipment and evaluation of the parts acquisition costs. A flow diagram with all components was developed, a complete list of all necessary components was presented, and an estimate of the acquisition of these parts in Brazil was reported. The stages of construction along with the importance of each component in the equipment were discussed. An equipment designs were presented as a result of the current work that serve as a basis for consulting future work on the development of new equipment.115CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP302423/2015-0Sem informaçãoSem informaçã

Enzymatic synthesis of galactooligosaccharides using pressurised fluids as reaction medium

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)This work is focussed on the evaluation of enzymatic synthesis of galactooligosaccharides (GOS) catalysed by beta-galactosidase from permeabilized cell of Kluyveromyces marxianus in different compressed fluids. The experiments were performed in a batch mode reactor, where the effects of enzyme and lactose concentrations and system pressure were evaluated. Results showed significant differences in the maximum GOS concentration among the experimental conditions, where the lactose concentration and enzyme activity were found to be statistically significant. Comparing the GOS productions in the three reaction systems, the supercritical CO2 led to the best results, where the maximum production was 83 gl(-1) while, for propane and n-butane, values of 63.0 and 75.0 gl(-1) were verified, respectively. According to the kinetic results of GOS production in supercritical CO2, a promising route is noted for this system, since the competition between synthesis and hydrolysis was less pronounced compared to the other tested fluids. (C) 2012 Elsevier Ltd. All rights reserved.133414081413Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Partial Characterization of Inulinases Obtained by Submerged and Solid-State Fermentation Using Agroindustrial Residues as Substrates: A Comparative Study

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Inulinase belongs to an important class of enzymes as it can be used to produce high-fructose syrups by enzymatic hydrolysis of inulin and fructooligosaccharides, which has been used as functional food. This work aimed to carry out a partial characterization of the crude enzymatic extract of two different inulinases, obtained by solid-state fermentation (SSF) and submerged fermentation (SmF), using agroindustrial residues as substrates. The crude enzymatic extract obtained by SmF showed an optimal pH and temperature for hydrolytic activity of 4.5 and 55 A degrees C, respectively; and that obtained by SSF conducted to optimal pH and temperature of 5.0 and 55 A degrees C, respectively. Both enzymes presented high thermostability, with a D value of 230.4 h and 123.1 h for SmF and SSF, respectively. The inulinase produced by SmF showed highest stability at pH 4.4, while inulinase obtained by SSF was more stable at pH 4.8. The results showed that inulinase obtained by SmF is less susceptible to pH effect and the inulinase obtained by SSF is more resistant to higher temperatures.1603682693FAPERGSFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Precipitation of particles using combined high turbulence extraction assisted by ultrasound and supercritical antisolvent fractionation

High Turbulence Extraction Assisted by Ultrasound combined with Supercritical Antisolvent Fractionation (SAF) was done to intensify the recovery of bixin and phenolic compounds from semi-defatted annatto seeds. Annatto seeds are extensively used due to its colorant properties and bioactive potential in human health. Modifications were performed in the SAF experimental apparatus in order to prevent losses of product. Results show that supercritical fractionation resulted in particle yields between 72.2–80.5%, 20–50% phenolics, and 66% bixin.3549CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP302423/2015-088882.305824/2013-012015/13299-