15 research outputs found
In vitro determination of termal diffusivity characteristic coefficients of resin/ionomer dental restorative biomaterials
Hydrothermal treatment and butylphosphonic acid derived self-assembled monolayers for improving the surface chemistry and corrosion resistance of AZ61 magnesium alloy
Synthesis of macroporous poly(dimethylsiloxane) scaffolds for tissue engineering applications
Macroporous, biostable scaffolds with controlled porous architecture were prepared from poly(dimethylsiloxane) (PDMS) using sodium chloride particles (NaCl) and a solvent casting and particulate leaching (SCPL) technique. The effect of particulate size range and overall porosity on the resulting structure was evaluated. Results found 90% v/v scaffolds and particulate ranges above 100 µm to have the most optimal open framework and porosity. Resulting hydrophobic PDMS scaffolds were coated with fibronectin and evaluated as a platform for adherent cell culture using human mesenchymal stem cells. Biocompatibility of PDMS scaffolds was also evaluated in a rodent model, where implants were found to be highly biocompatibile and biostable, with positive extracellular matrix deposition throughout the scaffold. These results demonstrate the suitability of macroporous PDMS scaffolds for tissue engineering applications where strong integration with the host is desired
Biomimetic hydroxyapatite coating on glass coverslips for the assay of osteoclast activity in vitro
The osteoclast (OC) is the cell type responsible for the resorption of bone. The activity of this cell is important in the aetiology of a large number of skeletal pathologies, and also for the biocompatibility and osseointegration of orthopaedic implant materials. OC mediated acid hydrolysis of calcium phosphate from the bone matrix offers a prime means of studying the biology and activity of this cell type. We have developed a method of coating glass coverslips with a hydroxyapatite (HA)-like mineral, using a biomimetic approach. Hydroxylation followed by formation of a self assembled monolayer (SAM) using the surfactant triethoxysilylpropyl succinic anhydride (TESPSA), allowed biomimetic deposition of HA-like mineral from a simulated body fluid (SBF). The biocompatibility of the TESPSA SAM-HA coated glass coverslips was tested by culturing human mature OC present in samples of giant cell tumour of bone (GCT). Parameters of OC activity were assayed, including F-actin ring formation, release of calcium and formation of osteoclastic resorption pits, confirming that OC were able to attach to and resorb the coated surface. This approach for the preparation of HA coatings on glass coverslips could have wide applicability for the study of osteoclast behaviour in vitro.Asiri K. A. R. Wijenayak, Christopher B. Colby, Gerald J. Atkins and Peter Majewsk
Remineralization of partially demineralized dentine substrate based on a biomimetic strategy
10.1007/s10856-012-4550-5Journal of Materials Science: Materials in Medicine233733-742JSMM
Simple one-step method to produce titanium dioxide–polycaprolactone composite films with increased hydrophilicity, enhanced cellular interaction and improved degradation for skin tissue engineering
Human corneal keratocyte response to micro-and nano-gratings on chitosan and PDMS
10.1007/s12195-011-0186-7Cellular and Molecular Bioengineering43399-41