28 research outputs found
Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determined by multiparametric single cell mass cytometry analysis
Full text linkPotential factors involved in the causation of rhabdomyolysis following status asthmaticus
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Palladium-based mass tag cell barcoding with a doublet-filtering scheme and single-cell deconvolution algorithm
No full textReversibility of Defective Hematopoiesis Caused by Telomere Shortening in Telomerase Knockout Mice
No full textTracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche
Get PDFWhile implicated in therapeutic resistance, malignant progenitor cell cycle kinetics have been difficult to quantify in real-time. We developed an efficient lentiviral bicistronic fluorescent, ubiquitination-based cell cycle indicator reporter (Fucci2BL) to image live single progenitors on a defined niche coupled with cell cycle gene expression analysis. We have identified key differences in cell cycle regulatory gene expression and transit times between normal and chronic myeloid leukemia progenitors that may inform cancer stem cell eradication strategies
