14 research outputs found
The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells
No full textInternational audienceThis protocol presents recently developed methodologies for the differentiation of human pluripotent stem cells (hPSCs) into ureteric bud (UB) progenitor-like cells. Differentiation of human PSCs to UB progenitor-like cells allows for the generation of chimeric kidney cultures in which the human cells can self-assemble into chimeric 3D structures in combination with embryonic mouse kidney cells over a period of 18 d. UB progenitor-like cells are generated by a two-step process that combines in vitro commitment of human PSCs, whether embryonic stem cells (ESCs) or induced PSCs (iPSCs), under chemically defined culture conditions, with ex vivo cultures for the induction of 3D organogenesis. The models described here provide new opportunities for investigating human kidney development, modeling disease, evaluating regenerative medicine strategies, as well as for toxicology studies
The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor–like cells
No full textProspective multicenter randomized trial of fast ventricular tachycardia termination by prolonged versus conventional anti-tachyarrhythmia burst pacing in implantable cardioverter-defibrillator patients-Atp DeliVery for pAiNless ICD thErapy (ADVANCE-D) Trial results
No full textLearning from multi-paradigmatic sensitivity in cross-cultural management? Empirical and theoretical considerations
No full textRapid and Highly Efficient Generation of Induced Pluripotent Stem Cells from Human Umbilical Vein Endothelial Cells
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The metabolome of induced pluripotent stem cells reveals metabolic changes occurring in somatic cell reprogramming
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