The heterogeneity of the plasma membrane H+ ATPase response to auxin. An alternative way to analyse signal perception and transduction ?

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Adoptive transfer of regulatory T cells.

<p>Naïve mice that were transferred with different cells, were subsequently sensitized with CMP and CT, and finally challenged with CMP_ig (n = 5/group). <b>A</b>, Transfer of <i>in vitro</i>-differentiated Treg. Sorted naïve CD4⁺CD25^- spleen cells were stimulated with a-CD3/a-CD28, IL-2 and TGF-β for 5 days. CD25⁺FoxP3⁺ cells gated on CD4⁺ lymphocytes were quantified (histogram) by flow cytometry and transferred to naïve receptor mice. <b>B,</b> Skin test, symptoms scored following the oral challenge and serum CMP-specific IgE in receptor mice. a) Control mice: naïve mice only received PBS_iv; b) Control mice: naïve mice received PBS_iv and were sensitized; c) naïve mice received differentiated-Treg and were sensitized. <b>C,</b><i>In vivo</i>-induced Treg in mice that were orally given daily dose of CMP and then sensitized (CMP_prev). As control, donor mice received daily dose of PBS_ig and then were sensitized (PBS_prev). MLN cells were isolated from donor mice and FoxP3⁺ cells (gated from CD4⁺CD25⁺ lymphocytes) were evaluated and quantified by flow cytometry. <b>D</b>, CD25 depletion was performed on MLN cells and evaluated by flow cytometry. Depleted and not depleted cells were adoptively transferred to receptor mice, which were then sensitized. <b>E</b>, Skin test, symptoms scored following the oral challenge and serum CMP-specific IgE in receptor animals. a) Control mice: naïve recipient mice received PBS_iv and then were sensitized; b) Control mice: receptor mice that received MLN cells depleted with a-CD25 isolated from donor PBS_prev animals and then were sensitized; c) Receptor mice that received MLN cells depleted with a-CD25 isolated from donor CMP_prev animals and then were sensitized; d) Receptor mice that received MLN cells from PBS_prev animals treated with isotype control antibody and then were sensitized; e) Receptor mice that received MLN cells from CMP_prev animals treated with isotype control antibody and then were sensitized. Results are representative of two independent experiments with similar results. Two-way ANOVA was used because all the data had normal distribution and equal variances <i>***P</i><0.001, <i>**P</i><0.01, <i>*P</i><0.05.</p