Enhancement of CD93 expression and interleukin-8 (IL-8) production in the human monocyte-like cell line U937 in response to Taimatsu fermented rice germ gamma-amino butyric acid (GABA)

たいまつ米胚芽発酵GABAによる単球系細胞株(U937)表面上のCD93抗原の発現をモノクローナル抗体を用いた酵素抗体法およびフローサイトメトリー法で解析した。その結果、たいまつ米胚芽発酵GABAで培養したU937細胞はCD93(C1qRp)の発現が有意に増強した。また、CD93の発現増強はprotein kinase C(PKC)阻害剤であるGo6976で抑制された。さらに、たいまつ米胚芽発酵GABAで培養したU937細胞はインターロイキン-8(IL-8)の産生も有意に増強した。以上の結果から、たいまつ米胚芽発酵GABAにはヒトの免疫応答を増強する作用があることがわかった。We examined the modulation of CD93 (receptor for complement component 1, subcomponent qphagocytosis; C1qRp) expression in the human monocyte-like cell line U937 cultured with Taimatsufermented rice germ gamma-amino butyric acid (GABA) using CD93 monoclonal antibodies (mAbs), aglutaraldehyde-fixed cellular enzyme immunoassay (GCEIA), and flow cytometry. The expression ofCD93 in the U937 cells cultured with Taimatsu fermented rice germ GABA was significantly enhanced (P<0.01), compared with that in the control cells (cultured without Taimatsu fermented rice germ GABA) at 24 hrs. This enhancement of CD93 expression was dose-dependent and timedependent. The enhancement of CD93 expression was markedly blocked in the presence of the protein kinase C (PKC) inhibitor Go6976. In addition, the U937 cells cultured with Taimatsu fermented rice germ GABA significantly enhanced (P<0.01) the production of interleukin-8 (IL-8) in the culture supernatants. Together, these findings strongly indicated that Taimatsu fermented rice germ GABA has immunopotentiator effects on some human immune responses

Actions of Taimatsu fermented rice germ solution on human immune responses

本研究では、我々が独自に開発したGABA およびIP6 含有たいまつ米胚芽発酵液(T-FRGS)のヒト末梢血単核球(PBMCs) に対する形態変化およびサイトカイン産生能を解析した。その結果、T-FRGS はPBMCs に形態変化(細胞間凝集)を誘導した。さらに、Enzyme immunoassay (EIA) を用いた解析から、T-FRGS はPBMCs からIL-1β、IL-6、IL-8、TNF-α、GM-CSF、G-CSF の産生を有意に増強させた(P<0.001)。以上の結果から、T-FRGS にはヒト免疫応答を増強させる作用があることがわかった。In this study, we examined the immunological actions of Taimatsu fermented rice germ solution (designated as T-FRGS), containing gamma-aminobutyric acid (GABA) and inositol hexaphosphate (IP6). The morphology of peripheral blood mononuclear cells (PBMCs) from normal healthy donors cultured with T-FRGS was dramatically altered (inducement of cell aggregation) as compared with that of the control cells (cultured without T-FRGS) at 24 hrs. In addition, the PBMCs from normal healthy donors cultured with T-FRGS showed strongly enhanced ability to produce some cytokines, such as interleukin (IL)-1 β ,IL-6, IL-8, tumor necrosis factor-α (TNF-α), granulocyte-macrophage colony stimulating factor (GM-CSF) and granulocyte colony stimulating factor (G-CSF) but not others, such as IL-2 or IL-12. Taken together, these findings clearly indicate that T-FRGS has stimulatory effects on some human immune responses

Inhibition of tumor-associated antigens secreted from cancer cell lines by Taimatsu fermented rice germ solution containing inositol hexaphosphate (IP6)

本研究では、我々が開発したIP6含有たいまつ米胚芽発酵液(T-IP6)の癌細胞株(MKN-45およびHepG2)から分泌される腫瘍関連抗原(CEAまたはPIVKA-II)に対する影響を化学発光および電気化学発光酵素免疫測定法で解析した。その結果、T-IP6で培養したMKN-45およびHepG2細胞から分泌されるCEAまたはPIVKA-IIは、細胞の形態変化を伴いながら有意に抑制された(P<0.01)。また、T-IP6によるCEAまたはPIVKA-IIの抑制作用は濃度依存性であった(P<0.05)。以上の結果から、T-IP6にはヒトの癌細胞を正常化させる作用があることがわかった。Inositol hexaphosphate (IP6), a naturally polyphosphorylated carbohydrate, has been reported to have significant anticancer activity against numerous tumors, such as colon, prostate, breast, liver and rhabdomyosarcomas. Recently, we established a new IP6-containing solution, designated as T-IP6, produced by Taimatsu fermented rice germ. In this study, we examined the inhibitory effects of tumor-associated antigens secreted from a gastric cancer cell line (MKN-45) and a hepatocellular carcinoma cell line (HepG2) exposed to T-IP6. The tumor-associated antigens, such ascarcinoembryonic antigen (CEA) and protein induced by vitamin K absence or antagonists-II (PIVKAII), secreted from these cancer cell lines cultured with T-IP6 were significantly inhibited (P<0.01), compared with those in control cells (cultured without T-IP6) at 72 hrs, and this inhibition was accompanied by morphological changes, respectively. In addition, T-IP6 inhibited CEA and PIVKA-II in a dose-dependent manner. These findings suggest that T-IP6 has an immunoinhibitory and normalization effect in human cancer cells

米胚芽発酵ギャバ(GABA)エキス末入りパンの作製

In this study, in order to promote intake of γ-aminobutyric acid (GABA) routinely and continuously, we prepared several functional breads containing different percentages (0%, 1%, 2% or 3%) of GABA, designated as Taimatsu GABA (T-GABA), produced by fermented rice germ, which has been shown to have various physiological actions. The dough containing T-GABA (1%) showed greater expansion as compared with that containing T-GABA (0%, 2% or 3%). The quantity of wet-type gluten decreased in a T-GABA percentage-dependent manner. The stickiness of wet-type gluten containing T-GABA (1%, 2% or 3%) was decreased as compared with that of the control bread (T-GABA; 0%). Although the weight of the bread containing GABA (1%, 2% or 3%) was not altered as compared with that of the control bread (T-GABA; 0%) after baking, the height of the bread containing T-GABA (3%) was the lowest. In the sensory evaluation, referees judged the bread containing T-GABA (1%) as showing a slightly better level of taste, feeling of softness, crust, crumb and fragrance as compared with that of the control bread (T-GABA; 0%). Together, these findings indicate that breads containing T-GABA are of functionally high quality and value, and we propose to introduce these breads into some long-term care health facilities

Agglutination et précipitation spécifiques

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Contribution à l'étude de la lyse des cellules sanguines par la staphylotoxine

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Quelques remarques sur l'organisation générale de la santé publique en suisse

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Contribution à l'étude des antigènes et des anticorps staphylococciques

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A propos de l'organisation en Suisse, de la protection de l'enfance et de la jeunesse

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Contribution à l'étude de l'adhésion moléculaire et de son intervention dans divers phénomènes biologiques

Doctorat en sciences médicalesinfo:eu-repo/semantics/nonPublishe