5 research outputs found
Histomorphologische und -morphometrische Untersuchungen von fruehen Knochenschaeden nach Inkorporation optimal kanzerogener Dosen von 239-Plutonium an maennlichen Ratten verschiedenen Alters
Primary breast cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up
The original local lymph node assay (LLNA) is based on the use of radioactive labelling to measure cell proliferation. Other endpoints for the assessment of proliferation are also authorized by the OECD Guideline 429 provided there is appropriate scientific support, including full citations and description of the methodology (OECD, 2002. OECD Guideline for the Testing of Chemicals; Skin Sensitization: Local Lymph Node Assay, Guideline 429. Paris, adopted 24th April 2002.). Here, we describe the outcome of the second round of an inter-laboratory validation of alternative endpoints in the LLNA conducted in nine laboratories in Europe. The validation study was managed and supervised by the Swiss Agency for Therapeutic Products (Swissmedic) in Bern. Ear-draining lymph node (LN) weight and cell counts were used to assess LN cell proliferation instead of [3H]TdR incorporation. In addition, the acute inflammatory skin reaction was measured by ear weight determination of circular biopsies of the ears to identify skin irritation properties of the test items. The statistical analysis was performed in the department of statistics at the university of Bern. Similar to the EC3 values defined for the radioactive method, threshold values were calculated for the endpoints measured in this modification of the LLNA. It was concluded that all parameters measured have to be taken into consideration for the categorisation of compounds due to their sensitising potencies. Therefore, an assessment scheme has been developed which turned out to be of great importance to consistently assess sensitisation versus irritancy based on the data of the different parameters. In contrast to the radioactive method, irritants have been picked up by all the laboratories applying this assessment scheme
Influence of concentration in phosphoric acid treatment of titanium oxide and their powder properties
Priority setting for the evaluation of existing chemicals the approach of the German advisory committee on existing chemicals of environmental relevance (BUA).
A method is described, which allows a rapid classification of chemicals. Based on data sets, exposure and ecotoxic and toxic effects are evaluated to enable overall classification into the following groups: I) Chemicals probably hazardous for man or environment, II) Chemicals where data are not sufficient for classification, and III) Chemicals probably non hazardous. Chemicals considered to be of relevance only at the workplace are classified seperately (Group IV)