Boundary of oxidative and overflow metabolism (boom) controller for CHO cell feed control

There is limited literature for CHO cell cultures with low batch glucose concentrations (Gowtham et al. 2017; Lu et al. 2005; Wong et al. 2005). Work like Xu et al. (2016) and Berry et al. (2016) have shown positive results for controlled fed-batch cultures at low glucose concentrations following standard high glucose (5-6 g/L) batch cultures. However, the Xu et al. (2016) and Berry et al. (2016) approaches still accumulate lactate. Controlling glucose earlier could potentially avoid lactate accumulation and lead to even greater improvements in culture outcomes. The objective of this project was to develop an advanced feed controller for CHO cell cultures that maximizes cell growth by maintaining the culture in a state of maximal oxidative metabolism while minimizing overflow metabolism. The Boundary of Oxidative and Overflow Metabolism (BOOM) controller periodically manipulates the feed rate while monitoring online signals to gauge the remaining oxidative “space”, in order to decide whether feed can be increased while remaining in oxidative metabolism. The Oxygen Uptake Rate (OUR) is the primary signal of interest, since it plateaus when a culture shifts from oxidative to overflow metabolism, encoding vital information about metabolic state. This project’s approach is different from past work in that the batch glucose concentrations is much lower (on the order of 1 g/L), the glucose and/or glutamine feeding begins very early in the process, and glucose feed is triggered/controlled by the off-gas sensing of the metabolic state instead of a targeted glucose concentration. During early runs several chemistry effects were observed directly due to the bolus feed additions interfering with the media-dissolved gas equilibrium. For example, a bolus feed that only contained 5 mM bicarbonate, resulted in an observed short sharp decrease in CO2 off-gas as the feed absorbed CO2 from the 5% CO2 sparge gas. Continuous feeding was introduced in subsequent runs as a means to mitigate disrupting the media-dissolved gas-equilibrium and disturbing the off-gas sensing. In order to have effective continuous feeding, the feed pump used a pulse width modulation (PWM) with a 10-minute period to allow extremely low effective feed rates required for the 1-L vessel. Two runs were used to demonstrate that the PWM feed pump could provide these very low pump feed rates for the 1-L vessel containing as little as 500 mL media. Initial glucose concentrations between 0.6 to 2.0 g/L were used (compared to 8 g/L glucose in the standard media formulation). Feedings have started between 6- and 20-hour post-inoculation. Distinct qualitative and quantitative differences have been observed in the corresponding oxygen uptake rate (OTR) responses due to the feeding spikes, suggesting that metabolic state can be detected. The development of the state estimator to control glucose feeding will be presented

Towards a greater dialogue on disability between Muslims and Christians

Attitudes to disability and disabled people by Muslims – focusing on attitudes in the Middle East and North Africa - and Christians – focusing on the West (here taken to mean Europe, North America and Australasia) - were examined through a grounded theory literature search, with the study being divided into three phases of reading and analysis. The aims of study were to develop a dialogue on disability between the two cultures, to inform an understanding of the attitudes to disability in the two cultures, and to inform cultural practice in promoting support and equality in both cultures. The study finds that Islam and Christianity have much in common and are a force for good in promoting and developing disability equality in both Muslim and Christian cultures

Special needs education in the United Arab Emirates (UAE): a systems perspective

In order to obtain maximum benefits from the educational system, it is imperative that the system should work as a unified coherent unit. Gaad, Arif and Scott (2006) conducted the systems analysis of the United Arab Emirates’ (UAE) general education system. They analysed three components of the system in order to assess the development, delivery and evaluation. This paper utilises the same framework to analyse the special needs education (SEN) system in the UAE. Data for the research were collected through observations and semi-structured interviews. Analysis of the data identifies some possibilities for improvement. In the area of development, more teaching and supplemental material specific to special needs education needs to be developed. Currently, teachers are working with what is known as the ‘para curriculum’. It is based on the regular textbook; with chapters that are difficult being simplified or removed. In the area of delivery, more training would be beneficial to teachers. The monitoring and evaluation component's analysis points out that the evaluation instrument used currently needs to be modified for special needs education monitoring and additional resources in the area of special needs education would required

Systems analysis of the UAE education system

Purpose – This purpose of this paper is to examine the organization of the UAE educational system, its components, their goals, and finally their effectiveness. Design/methodology/approach – Three elements of the UAE education system were examined: the development; delivery; and evaluation and feedback. Findings – On analysis, it was found that there is poor alignment among what the system was developed for, how it was delivered, and what was evaluated. In order to align this system significant efforts are needed to ensure that extensive documentation in terms of teachers' guides, training sessions for teachers and supervisors, and relevant evaluation instruments must be designed with systems thinking as the guide. Research limitations/implications – An orientation to goals rather than textbooks, to systems thinking, and to the vision developed for the system (and perhaps some form of strategic feedback to the respective committees established in the structural framework delineated by the ministry) might be worth consideration to erase the misalignment indicated by this research. Originality/value – This paper will be of interest to those involved in education and in particular, those involved in the education system within the UAE